LOCUS       sequence-378475-       13794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains an array of guide RNAs targeting LTR5Hs elements.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Fuentes DR, Swigut T, Wysocka J
  TITLE     Systematic perturbation of retroviral LTRs reveals widespread 
            long-range effects on human gene regulation.
  JOURNAL   Elife. 2018 Aug 2;7. pii: 35989. doi: 10.7554/eLife.35989.
  PUBMED    30070637
REFERENCE   2  (bases 1 to 13794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-08-2"; volume: "7. pii: 35989. doi"; pages: " 
            10.7554/eLife.35989"
COMMENT     Sequence Label: LTR5HS S. pyogenes scaffold CARGO array
FEATURES             Location/Qualifiers
     source          1..13794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(366..377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      1210..1219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1216..2019
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1279..1298)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1889..1908
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2031..2152
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2068..2087)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2122..2141
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   2915..2949
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3440..3456)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3440..3456)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3453..3475)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3464..3480
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3488..3494,3495..3512,3513..3518))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3533..3554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3671..3688)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3842..4430)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3922..3941)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4601..5392,5393..5461))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5224..5243
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5462..5566)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5592..6047)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5729..5750)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5941..5960
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6174..6196
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6188..6205
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6189..6205
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   6335..6397
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    6828..6945
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        11917..12202
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        12204..12480
                     /label=chicken beta-actin promoter
     intron          12481..12708
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      12720..12729
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12726..13436
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(12871..12889)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(13461..13478)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13467..13674
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    join(13689..13794,1..483)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(13742..13762)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
       61 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
      121 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
      181 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
      241 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
      301 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
      361 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
      421 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
      481 cgcctggaat ttcaccggcg accgattcct cccgataagt aaattacaga aaagctcagg
      541 ttaaatgtaa gaggatctta aatattgatt tggtttttgt cttaatggtg cccggcgtat
      601 ttatacgaac cattaaattt cactagaccg tttgtagtca gtggtgaacg ctgcatgacc
      661 cacgctctca aagtgagttg tgttgctttg cgggaatcca gcagtcacat taatgctgga
      721 aaaagttcag gcaactagtg tactttgtag cggggtccgg gagaggctgg ggtctagagt
      781 ggcgatggga cgaaagtgta aagaaagttt tcggtgtgtg tgtgtgtgtg tgtgtgtgtg
      841 tgtgtgtgtg tgtaggtagg taggtagaaa ctgcatcctc tgcttgtgta aattcggtta
      901 caagtctgta acagaggtac tgagatgtga ctgagtctca aggccaggcg atcgggattc
      961 tgaagaggca tttgcataac tgagcaagag tctttgcccc acccttccac gccgacccaa
     1021 gacaagggga cagctctggg tgggtcacct gaagggccag gggcccgccc acatccccgc
     1081 ccacacccac cttgagcgct tctcattggg cattccagcc tacccagctc ggagttagtt
     1141 actccgtaag tgtggccgga acagagttcg tccatctaaa aagggagggg accggcgaac
     1201 acatctagag ccaccatggg atcggccatt gaacaagatg gattgcacgc aggttctccg
     1261 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     1321 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     1381 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     1441 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     1501 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     1561 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     1621 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     1681 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     1741 aggctcaagg cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc
     1801 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     1861 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     1921 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     1981 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa gaactaacta aacttgttta
     2041 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     2101 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     2161 ggaattgact caaatgatgt caattagtct atcagaagct catctggtct cccttccggg
     2221 ggacaagaca tccctgttta atatttaaac agcagtgttc ccaaactggg ttcttatatc
     2281 ccttgctctg gtcaaccagg ttgcagggtt tcctgtcctc acaggaacga agtccctaaa
     2341 gaaacagtgg cagccaggtt tagccccgga attgactgga ttcctttttt agggcccatt
     2401 ggtatggctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc
     2461 gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc
     2521 cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg
     2581 ccccctagcg ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct
     2641 gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata
     2701 acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca
     2761 ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac
     2821 gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg
     2881 ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc
     2941 tttctagggt taatctagct gcatcaggat catatcgtcg ggtctttttt ccggctcagt
     3001 catcgcccaa gctggcgcta tctgggcatc ggggaggaag aagcccgtgc cttttcccgc
     3061 gaggttgaag cggcatggaa agagtttgcc gaggatgact gctgctgcat tgacgttgag
     3121 cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt
     3181 gaactgttcg ttcaggccac ctgggatacc agttcgtcgc ggcttttccg gacacagttc
     3241 cggatggtca gcccgaagcg catcagcaac ccgaacaata ccggcgacag ccggaactgc
     3301 cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg gatattacgt cagcgaggac
     3361 gggtatcctg gctggatgcc gcagaaatgg acatggatac cccgtgagtt acccggcggg
     3421 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     3481 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     3541 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     3601 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     3661 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     3721 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     3781 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3841 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3901 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3961 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4021 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4081 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4141 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4201 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4261 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     4321 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4381 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4441 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4501 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4561 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4621 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4681 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4741 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4801 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4861 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     4921 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4981 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     5041 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     5101 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     5161 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     5221 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     5281 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     5341 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     5401 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     5461 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     5521 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     5581 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     5641 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     5701 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     5761 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     5821 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     5881 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     5941 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     6001 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     6061 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     6121 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     6181 acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt ttgaacccgt
     6241 ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc agatgaagat
     6301 gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc atattgtgac
     6361 gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc tgtatatcga
     6421 ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt acatactaat
     6481 aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa caaaaactca
     6541 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     6601 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     6661 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     6721 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     6781 acacctgggg ggatacgggg aaaaggcctc cacggccaga ctagaacttt taaaagaaaa
     6841 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     6901 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tcacgagact
     6961 agcctcgagg gatcccgtac gttcgaaacg cgtgcctagc gaattcaccg gtagatctgc
     7021 tagcggcgcg ccggtaccca cgtgcacatg tgtttaagag ctatgctgga aacagcatag
     7081 caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     7141 ttttctcgag tactaggatc cattaggctt tcccatgatt ccttcatatt tgcatatacg
     7201 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt
     7261 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
     7321 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
     7381 atatatcttg tggaaaggac gaaacaccgt actaagggaa ctcaggttta agagctatgc
     7441 tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac
     7501 cgagtcggtg ctttttttct cgagtactag gatccattag gctttcccat gattccttca
     7561 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac
     7621 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
     7681 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
     7741 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgaaaaccg ccttagggcg
     7801 tttaagagct atgctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact
     7861 tgaaaaagtg gcaccgagtc ggtgcttttt ttctcgagta ctaggatcca ttaggctttc
     7921 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attggaatta
     7981 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc
     8041 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa
     8101 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgaaa
     8161 actgccttag ggcgtttaag agctatgctg gaaacagcat agcaagttta aataaggcta
     8221 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctcg agtactagga
     8281 tccattaggc tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
     8341 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
     8401 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     8461 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     8521 acgaaacacc gagcccgaca cccgtaagtt taagagctat gctggaaaca gcatagcaag
     8581 tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
     8641 ctcgagtact aggatccatt aggctttccc atgattcctt catatttgca tatacgatac
     8701 aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa
     8761 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     8821 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     8881 atcttgtgga aaggacgaaa caccgaggag aaaaccacct tgtttaagag ctatgctgga
     8941 aacagcatag caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     9001 tcggtgcttt ttttctcgag tactaggatc cattaggctt tcccatgatt ccttcatatt
     9061 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
     9121 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
     9181 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
     9241 tcttggcttt atatatcttg tggaaaggac gaaacaccgt accatctcgg agagggttta
     9301 agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa
     9361 aaagtggcac cgagtcggtg ctttttttct cgagtactag gatccattag gctttcccat
     9421 gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt
     9481 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
     9541 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
     9601 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgaaccttg
     9661 gacaataccg tttaagagct atgctggaaa cagcatagca agtttaaata aggctagtcc
     9721 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttctcgagta ctaggatcca
     9781 ttaggctttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
     9841 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
     9901 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
     9961 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
    10021 aacaccgtaa agggtctgtg ctggtttaag agctatgctg gaaacagcat agcaagttta
    10081 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctcg
    10141 agtactagga tccattaggc tttcccatga ttccttcata tttgcatata cgatacaagg
    10201 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
    10261 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
    10321 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
    10381 tgtggaaagg acgaaacacc ggacattcca ttgcccagtt taagagctat gctggaaaca
    10441 gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
    10501 tgcttttttt ctcgagtact aggatccatt aggctttccc atgattcctt catatttgca
    10561 tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat
    10621 attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa
    10681 attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt
    10741 ggctttatat atcttgtgga aaggacgaaa caccgataga gaaagaaaag tgtttaagag
    10801 ctatgctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa cttgaaaaag
    10861 tggcaccgag tcggtgcttt ttttctcgag tactaggatc cattaggctt tcccatgatt
    10921 ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat taatttgact
    10981 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
    11041 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
    11101 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg aaacgcccac
    11161 aggtggttta agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta
    11221 tcaacttgaa aaagtggcac cgagtcggtg ctttttttct cgagtactag gatccattag
    11281 gctttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
    11341 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
    11401 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
    11461 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
    11521 ccgaagcttt cttgcttagg tttaagagct atgctggaaa cagcatagca agtttaaata
    11581 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttctcgagta
    11641 ctaggatcca ttaggctttc ccatgattcc ttcatatttg catatacgat acaaggctgt
    11701 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
    11761 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
    11821 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
    11881 gaaaggacga aacaccgttt tttttctaga ggtacccgtt acataactta cggtaaatgg
    11941 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaatagtaa cgccaatagg
    12001 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
    12061 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
    12121 ctggcattgt gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
    12181 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
    12241 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
    12301 gatgggggcg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
    12361 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
    12421 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
    12481 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
    12541 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
    12601 ccgggctgta attagctgag caagaggtaa gggtttaagg gatggttggt tggtggggta
    12661 ttaatgttta attacctgga gcacctgcct gaaatcactt tttttcaggt tggaccggtg
    12721 ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
    12781 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
    12841 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
    12901 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
    12961 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
    13021 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
    13081 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
    13141 ccgtaatgca gaaaaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
    13201 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
    13261 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
    13321 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
    13381 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaggaat
    13441 tcctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
    13501 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
    13561 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
    13621 ggggcaggac agcaaggggg aggattggga agagaatagc aggcatgctg gggagcggcc
    13681 gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
    13741 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catg
//
