LOCUS       sequence-378476-       13664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains an array of guide RNAs targeting LTR5Hs elements.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13664)
  AUTHORS   Fuentes DR, Swigut T, Wysocka J
  TITLE     Systematic perturbation of retroviral LTRs reveals widespread 
            long-range effects on human gene regulation.
  JOURNAL   Elife. 2018 Aug 2;7. pii: 35989. doi: 10.7554/eLife.35989.
  PUBMED    30070637
REFERENCE   2  (bases 1 to 13664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-08-2"; volume: "7. pii: 35989. doi"; pages: " 
            10.7554/eLife.35989"
COMMENT     Sequence Label: LTR5HS S. aureus scaffold CARGO array
FEATURES             Location/Qualifiers
     source          1..13664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(124..228)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(254..709)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(391..412)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     603..622
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     836..858
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     850..867
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     851..867
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   997..1059
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    1490..1607
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        1714..1789
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_RNA        2078..2153
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_RNA        2442..2517
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_RNA        2806..2881
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_RNA        3170..3245
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_RNA        3534..3609
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_RNA        3898..3973
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_RNA        4262..4337
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_RNA        4626..4701
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_RNA        4990..5065
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_RNA        5354..5429
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_RNA        5718..5793
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_RNA        6082..6157
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     enhancer        6449..6734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        6736..7012
                     /label=chicken beta-actin promoter
     intron          7013..7240
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      7252..7261
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7258..7968
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7403..7421)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7993..8010)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7999..8206
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    8221..8809
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8274..8294)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8692..8703)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      9536..9545
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9542..10345
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9605..9624)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10215..10234
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10357..10478
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10394..10413)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10448..10467
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   11241..11275
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(11766..11782)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11766..11782)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11779..11801)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11790..11806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11814..11820,11821..11838,11839..11844))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11859..11880
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11997..12014)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12168..12756)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12248..12267)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12927..13664,1..54,55..123))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13550..13569
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
       61 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      121 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
      181 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
      241 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
      301 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
      361 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
      421 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
      481 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
      541 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
      601 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
      661 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
      721 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
      781 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
      841 tcacgacgtt gtaaaacgac ggccagtgag cgcgcctcgt tcattcacgt ttttgaaccc
      901 gtggaggacg ggcagactcg cggtgcaaat gtgttttaca gcgtgatgga gcagatgaag
      961 atgctcgaca cgctgcagaa cacgcagcta gattaaccct agaaagataa tcatattgtg
     1021 acgtacgtta aagataatca tgtgtaaaat tgacgcatgt gttttatcgg tctgtatatc
     1081 gaggtttatt tattaatttg aatagatatt aagttttatt atatttacac ttacatacta
     1141 ataataaatt caacaaacaa tttatttatg tttatttatt tattaaaaaa aacaaaaact
     1201 caaaatttct tctataaagt aacaaaactt ttatgaggga cagccccccc ccaaagcccc
     1261 cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg
     1321 ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca
     1381 cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc
     1441 agacacctgg ggggatacgg ggaaaaggcc tccacggcca gactagaact tttaaaagaa
     1501 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     1561 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc gatcacgaga
     1621 ctagcctcga gggatcccgt acgttcgaaa cgcgtgccta gcgaattcac cggtagatct
     1681 gctagcggcg cgccggtacc cacgtgcaca tgtgttatag tactctggaa acagaatcta
     1741 ctataacaag gcaaaatgcc gtgtttatct cgtcaacttg ttggcgagat ttttttctcg
     1801 agtactagga tccattaggc tttcccatga ttccttcata tttgcatata cgatacaagg
     1861 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
     1921 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     1981 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2041 tgtggaaagg acgaaacacc gtactaaggg aactcaggtt atagtactct ggaaacagaa
     2101 tctactataa caaggcaaaa tgccgtgttt atctcgtcaa cttgttggcg agattttttt
     2161 ctcgagtact aggactgatt aggctttccc atgattcctt catatttgca tatacgatac
     2221 aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa
     2281 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     2341 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     2401 atcttgtgga aaggacgaaa caccgaaaac cgccttaggg cgttatagta ctctggaaac
     2461 agaatctact ataacaaggc aaaatgccgt gtttatctcg tcaacttgtt ggcgagattt
     2521 ttttctcgag tactaggatc cattaggctt tcccatgatt ccttcatatt tgcatatacg
     2581 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt
     2641 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
     2701 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
     2761 atatatcttg tggaaaggac gaaacaccga aaactgcctt agggcgttat agtactctgg
     2821 aaacagaatc tactataaca aggcaaaatg ccgtgtttat ctcgtcaact tgttggcgag
     2881 atttttttct cgagtactag gatccattag gctttcccat gattccttca tatttgcata
     2941 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
     3001 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
     3061 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
     3121 ctttatatat cttgtggaaa ggacgaaaca ccgagcccga cacccgtaag ttatagtact
     3181 ctggaaacag aatctactat aacaaggcaa aatgccgtgt ttatctcgtc aacttgttgg
     3241 cgagattttt ttctcgagta ctaggatcca ttaggctttc ccatgattcc ttcatatttg
     3301 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag
     3361 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     3421 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     3481 ttggctttat atatcttgtg gaaaggacga aacaccgagg agaaaaccac cttgttatag
     3541 tactctggaa acagaatcta ctataacaag gcaaaatgcc gtgtttatct cgtcaacttg
     3601 ttggcgagat ttttttctcg agtactagga tccattaggc tttcccatga ttccttcata
     3661 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac
     3721 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     3781 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
     3841 tttcttggct ttatatatct tgtggaaagg acgaaacacc gtaccatctc ggagagggtt
     3901 atagtactct ggaaacagaa tctactataa caaggcaaaa tgccgtgttt atctcgtcaa
     3961 cttgttggcg agattttttt ctcgagtact aggatccatt aggctttccc atgattcctt
     4021 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
     4081 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     4141 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     4201 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgaacct tggacaatac
     4261 cgttatagta ctctggaaac agaatctact ataacaaggc aaaatgccgt gtttatctcg
     4321 tcaacttgtt ggcgagattt ttttctcgag tactaggatc cattaggctt tcccatgatt
     4381 ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat taatttgact
     4441 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
     4501 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
     4561 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgt aaagggtctg
     4621 tgctggttat agtactctgg aaacagaatc tactataaca aggcaaaatg ccgtgtttat
     4681 ctcgtcaact tgttggcgag atttttttct cgagtactag gatccattag gctttcccat
     4741 gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt
     4801 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
     4861 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
     4921 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccggacattc
     4981 cattgcccag ttatagtact ctggaaacag aatctactat aacaaggcaa aatgccgtgt
     5041 ttatctcgtc aacttgttgg cgagattttt ttctcgagta ctaggatcca ttaggctttc
     5101 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attggaatta
     5161 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc
     5221 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa
     5281 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgata
     5341 gagaaagaaa agtgttatag tactctggaa acagaatcta ctataacaag gcaaaatgcc
     5401 gtgtttatct cgtcaacttg ttggcgagat ttttttctcg agtactagga tccattaggc
     5461 tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga
     5521 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
     5581 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
     5641 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
     5701 ggaaacgccc acaggtggtt atagtactct ggaaacagaa tctactataa caaggcaaaa
     5761 tgccgtgttt atctcgtcaa cttgttggcg agattttttt ctcgagtact aggatccatt
     5821 aggctttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
     5881 tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
     5941 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
     6001 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
     6061 caccgaagct ttcttgctta ggttatagta ctctggaaac agaatctact ataacaaggc
     6121 aaaatgccgt gtttatctcg tcaacttgtt ggcgagattt ttttctcgag tactaggatc
     6181 cattaggctt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
     6241 taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
     6301 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
     6361 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     6421 gaaacaccgt tttttttcta gaggtacccg ttacataact tacggtaaat ggcccgcctg
     6481 gctgaccgcc caacgacccc cgcccattga cgtcaatagt aacgccaata gggactttcc
     6541 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     6601 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     6661 gtgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     6721 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
     6781 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
     6841 cggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
     6901 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
     6961 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc
     7021 tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
     7081 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
     7141 taattagctg agcaagaggt aagggtttaa gggatggttg gttggtgggg tattaatgtt
     7201 taattacctg gagcacctgc ctgaaatcac tttttttcag gttggaccgg tgccaccatg
     7261 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     7321 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     7381 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     7441 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     7501 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     7561 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     7621 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     7681 cagaaaaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     7741 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     7801 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     7861 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     7921 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtagga attcctagag
     7981 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     8041 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     8101 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     8161 acagcaaggg ggaggattgg gaagagaata gcaggcatgc tggggagcgg ccgcgtcgac
     8221 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     8281 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     8341 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     8401 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     8461 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     8521 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     8581 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     8641 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     8701 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     8761 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaatttca
     8821 ccggcgaccg attcctcccg ataagtaaat tacagaaaag ctcaggttaa atgtaagagg
     8881 atcttaaata ttgatttggt ttttgtctta atggtgcccg gcgtatttat acgaaccatt
     8941 aaatttcact agaccgtttg tagtcagtgg tgaacgctgc atgacccacg ctctcaaagt
     9001 gagttgtgtt gctttgcggg aatccagcag tcacattaat gctggaaaaa gttcaggcaa
     9061 ctagtgtact ttgtagcggg gtccgggaga ggctggggtc tagagtggcg atgggacgaa
     9121 agtgtaaaga aagttttcgg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgta
     9181 ggtaggtagg tagaaactgc atcctctgct tgtgtaaatt cggttacaag tctgtaacag
     9241 aggtactgag atgtgactga gtctcaaggc caggcgatcg ggattctgaa gaggcatttg
     9301 cataactgag caagagtctt tgccccaccc ttccacgccg acccaagaca aggggacagc
     9361 tctgggtggg tcacctgaag ggccaggggc ccgcccacat ccccgcccac acccaccttg
     9421 agcgcttctc attgggcatt ccagcctacc cagctcggag ttagttactc cgtaagtgtg
     9481 gccggaacag agttcgtcca tctaaaaagg gaggggaccg gcgaacacat ctagagccac
     9541 catgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     9601 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     9661 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     9721 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     9781 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     9841 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     9901 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     9961 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
    10021 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
    10081 catgcccgac ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
    10141 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
    10201 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
    10261 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
    10321 tcgccttctt gacgagttct tctgaagaac taactaaact tgtttattgc agcttataat
    10381 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10441 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggaa ttgactcaaa
    10501 tgatgtcaat tagtctatca gaagctcatc tggtctccct tccgggggac aagacatccc
    10561 tgtttaatat ttaaacagca gtgttcccaa actgggttct tatatccctt gctctggtca
    10621 accaggttgc agggtttcct gtcctcacag gaacgaagtc cctaaagaaa cagtggcagc
    10681 caggtttagc cccggaattg actggattcc ttttttaggg cccattggta tggctttttc
    10741 cccgtatccc cccaggtgtc tgcaggctca aagagcagcg agaagcgttc agaggaaagc
    10801 gatcccgtgc caccttcccc gtgcccgggc tgtccccgca cgctgccggc tcggggatgc
    10861 ggggggagcg ccggaccgga gcggagcccc gggcggctcg ctgctgcccc ctagcggggg
    10921 agggacgtaa ttacatccct gggggctttg ggggggggct gtccctgata tctataacaa
    10981 gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa tataattatc
    11041 gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt gactcacgcg
    11101 gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct cacgggagct
    11161 ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat ttagaaagag
    11221 agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc tagggttaat
    11281 ctagctgcat caggatcata tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg
    11341 gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc
    11401 atggaaagag tttgccgagg atgactgctg ctgcattgac gttgagcgaa aacgcacgtt
    11461 taccatgatg attcgggaag gtgtggccat gcacgccttt aacggtgaac tgttcgttca
    11521 ggccacctgg gataccagtt cgtcgcggct tttccggaca cagttccgga tggtcagccc
    11581 gaagcgcatc agcaacccga acaataccgg cgacagccgg aactgccgtg ccggtgtgca
    11641 gattaatgac agcggtgcgg cgctgggata ttacgtcagc gaggacgggt atcctggctg
    11701 gatgccgcag aaatggacat ggataccccg tgagttaccc ggcgggcgcg cttggcgtaa
    11761 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    11821 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    11881 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    11941 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    12001 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    12061 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    12121 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    12181 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    12241 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    12301 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    12361 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    12421 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    12481 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    12541 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    12601 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    12661 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    12721 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    12781 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    12841 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    12901 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    12961 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    13021 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    13081 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    13141 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    13201 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    13261 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    13321 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    13381 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    13441 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    13501 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    13561 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    13621 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccac
//
