LOCUS       sequence-378477-       13844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains an array of nontargeting guide RNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13844)
  AUTHORS   Fuentes DR, Swigut T, Wysocka J
  TITLE     Systematic perturbation of retroviral LTRs reveals widespread 
            long-range effects on human gene regulation.
  JOURNAL   Elife. 2018 Aug 2;7. pii: 35989. doi: 10.7554/eLife.35989.
  PUBMED    30070637
REFERENCE   2  (bases 1 to 13844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-08-2"; volume: "7. pii: 35989. doi"; pages: " 
            10.7554/eLife.35989"
COMMENT     Sequence Label: nontarget S. pyogenes scaffold CARGO array
FEATURES             Location/Qualifiers
     source          1..13844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(258..362)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(388..843)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(525..546)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     737..756
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     970..992
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     984..1001
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     985..1001
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1131..1193
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    1624..1741
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6761..7046
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          7327..7554
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      7566..7575
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7572..8282
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7717..7735)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(8307..8324)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8313..8520
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    8535..9123
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8588..8608)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9006..9017)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      9850..9859
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9856..10659
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9919..9938)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10529..10548
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10671..10792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10708..10727)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10762..10781
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   11555..11589
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(12080..12096)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12080..12096)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12093..12115)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12104..12120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12128..12134,12135..12152,12153..12158))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12173..12194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12311..12328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12482..13070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12562..12581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13241..13844,1..188,189..257))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
       61 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      121 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      181 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      241 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
      301 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
      361 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
      421 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
      481 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
      541 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
      601 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
      661 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
      721 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
      781 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
      841 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      901 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
      961 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcc tcgttcattc
     1021 acgtttttga acccgtggag gacgggcaga ctcgcggtgc aaatgtgttt tacagcgtga
     1081 tggagcagat gaagatgctc gacacgctgc agaacacgca gctagattaa ccctagaaag
     1141 ataatcatat tgtgacgtac gttaaagata atcatgtgta aaattgacgc atgtgtttta
     1201 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
     1261 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
     1321 aaaaaacaaa aactcaaaat ttcttctata aagtaacaaa acttttatga gggacagccc
     1381 ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc
     1441 cgcccggggc tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg
     1501 ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg
     1561 ctctttgagc ctgcagacac ctggggggat acggggaaaa ggcctccacg gccagactag
     1621 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1681 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1741 tatcgatcac gagactagcc tcgagggatc ccgtacgttc gaaacgcgtg cctagcgaat
     1801 tcaccggtag atctgctagc ggcgcgccgg tacccacgtg cacatgtgtt taagagctat
     1861 gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc
     1921 accgagtcgg tgcttttttt ctcgagtact aggatccatt aggctttccc atgattcctt
     1981 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
     2041 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     2101 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     2161 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgcggag taacaagcgg
     2221 acggagttta agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta
     2281 tcaacttgaa aaagtggcac cgagtcggtg ctttttttct cgagtactag gatccattag
     2341 gctttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
     2401 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     2461 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
     2521 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     2581 ccgcgagtgt tatacgcacc gttgtttaag agctatgctg gaaacagcat agcaagttta
     2641 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctcg
     2701 agtactagga tccattaggc tttcccatga ttccttcata tttgcatata cgatacaagg
     2761 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
     2821 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     2881 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2941 tgtggaaagg acgaaacacc gcaacgggtt ctcccggcta cgtttaagag ctatgctgga
     3001 aacagcatag caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     3061 tcggtgcttt ttttctcgag tactaggatc cattaggctt tcccatgatt ccttcatatt
     3121 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
     3181 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
     3241 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
     3301 tcttggcttt atatatcttg tggaaaggac gaaacaccgc aggagtcgcc gatacgcgtg
     3361 tttaagagct atgctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact
     3421 tgaaaaagtg gcaccgagtc ggtgcttttt ttctcgagta ctaggatcca ttaggctttc
     3481 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attggaatta
     3541 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc
     3601 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa
     3661 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgttc
     3721 acgtcgtctc gcgaccagtt taagagctat gctggaaaca gcatagcaag tttaaataag
     3781 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt ctcgagtact
     3841 aggatccatt aggctttccc atgattcctt catatttgca tatacgatac aaggctgtta
     3901 gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
     3961 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
     4021 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     4081 aaggacgaaa caccggtgtc ggattccgcc gcttagttta agagctatgc tggaaacagc
     4141 atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
     4201 ctttttttct cgagtactag gatccattag gctttcccat gattccttca tatttgcata
     4261 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
     4321 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
     4381 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
     4441 ctttatatat cttgtggaaa ggacgaaaca ccgcacgaac tcacaccgcg cgagtttaag
     4501 agctatgctg gaaacagcat agcaagttta aataaggcta gtccgttatc aacttgaaaa
     4561 agtggcaccg agtcggtgct ttttttctcg agtactagga tccattaggc tttcccatga
     4621 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga
     4681 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
     4741 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
     4801 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gcgctagtac
     4861 gctcctctat agtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt
     4921 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttctcgag tactaggatc
     4981 cattaggctt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
     5041 taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
     5101 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
     5161 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     5221 gaaacaccgt cgcgcttggg ttatacgctg tttaagagct atgctggaaa cagcatagca
     5281 agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     5341 ttctcgagta ctaggatcca ttaggctttc ccatgattcc ttcatatttg catatacgat
     5401 acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag atattagtac
     5461 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     5521 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     5581 atatcttgtg gaaaggacga aacaccgcta tctcgagtgg taatgcggtt taagagctat
     5641 gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc
     5701 accgagtcgg tgcttttttt ctcgagtact aggatccatt aggctttccc atgattcctt
     5761 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
     5821 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     5881 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     5941 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgaatcg actcgaactt
     6001 cgtgtgttta agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta
     6061 tcaacttgaa aaagtggcac cgagtcggtg ctttttttct cgagtactag gatccattag
     6121 gctttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
     6181 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     6241 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
     6301 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     6361 ccgcccgatg gactataccg aacgtttaag agctatgctg gaaacagcat agcaagttta
     6421 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctcg
     6481 agtactagga tccattaggc tttcccatga ttccttcata tttgcatata cgatacaagg
     6541 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
     6601 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     6661 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     6721 tgtggaaagg acgaaacacc gttttttttc tagaggtacc cgttacataa cttacggtaa
     6781 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata gtaacgccaa
     6841 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     6901 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     6961 ccgcctggca ttgtgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     7021 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
     7081 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
     7141 cagcgatggg ggcggggggg gggggggggg cgcgcgccrg gggggggggg ggggggsggg
     7201 gggsggggsg gggsgrggsg grgaggkgcg gcggcagcca atcagagcgg cgcgctccga
     7261 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
     7321 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
     7381 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
     7441 tctcctccgg gctgtaatta gctgagcaag aggtaagggt ttaagggatg gttggttggt
     7501 ggggtattaa tgtttaatta cctggagcac ctgcctgaaa tcactttttt tcaggttgga
     7561 ccggtgccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     7621 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     7681 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     7741 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     7801 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     7861 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     7921 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     7981 acggccccgt aatgcagaaa aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     8041 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     8101 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     8161 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     8221 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     8281 aggaattcct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     8341 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     8401 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     8461 tggggtgggg caggacagca agggggagga ttgggaagag aatagcaggc atgctgggga
     8521 gcggccgcgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     8581 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     8641 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     8701 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     8761 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     8821 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     8881 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     8941 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     9001 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     9061 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     9121 cgcctggaat ttcaccggcg accgattcct cccgataagt aaattacaga aaagctcagg
     9181 ttaaatgtaa gaggatctta aatattgatt tggtttttgt cttaatggtg cccggcgtat
     9241 ttatacgaac cattaaattt cactagaccg tttgtagtca gtggtgaacg ctgcatgacc
     9301 cacgctctca aagtgagttg tgttgctttg cgggaatcca gcagtcacat taatgctgga
     9361 aaaagttcag gcaactagtg tactttgtag cggggtccgg gagaggctgg ggtctagagt
     9421 ggcgatggga cgaaagtgta aagaaagttt tcggtgtgtg tgtgtgtgtg tgtgtgtgtg
     9481 tgtgtgtgtg tgtaggtagg taggtagaaa ctgcatcctc tgcttgtgta aattcggtta
     9541 caagtctgta acagaggtac tgagatgtga ctgagtctca aggccaggcg atcgggattc
     9601 tgaagaggca tttgcataac tgagcaagag tctttgcccc acccttccac gccgacccaa
     9661 gacaagggga cagctctggg tgggtcacct gaagggccag gggcccgccc acatccccgc
     9721 ccacacccac cttgagcgct tctcattggg cattccagcc tacccagctc ggagttagtt
     9781 actccgtaag tgtggccgga acagagttcg tccatctaaa aagggagggg accggcgaac
     9841 acatctagag ccaccatggg atcggccatt gaacaagatg gattgcacgc aggttctccg
     9901 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     9961 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
    10021 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
    10081 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
    10141 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
    10201 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
    10261 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
    10321 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
    10381 aggctcaagg cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc
    10441 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
    10501 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
    10561 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
    10621 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa gaactaacta aacttgttta
    10681 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    10741 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    10801 ggaattgact caaatgatgt caattagtct atcagaagct catctggtct cccttccggg
    10861 ggacaagaca tccctgttta atatttaaac agcagtgttc ccaaactggg ttcttatatc
    10921 ccttgctctg gtcaaccagg ttgcagggtt tcctgtcctc acaggaacga agtccctaaa
    10981 gaaacagtgg cagccaggtt tagccccgga attgactgga ttcctttttt agggcccatt
    11041 ggtatggctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc
    11101 gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc
    11161 cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg
    11221 ccccctagcg ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct
    11281 gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata
    11341 acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca
    11401 ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac
    11461 gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg
    11521 ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc
    11581 tttctagggt taatctagct gcatcaggat catatcgtcg ggtctttttt ccggctcagt
    11641 catcgcccaa gctggcgcta tctgggcatc ggggaggaag aagcccgtgc cttttcccgc
    11701 gaggttgaag cggcatggaa agagtttgcc gaggatgact gctgctgcat tgacgttgag
    11761 cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt
    11821 gaactgttcg ttcaggccac ctgggatacc agttcgtcgc ggcttttccg gacacagttc
    11881 cggatggtca gcccgaagcg catcagcaac ccgaacaata ccggcgacag ccggaactgc
    11941 cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg gatattacgt cagcgaggac
    12001 gggtatcctg gctggatgcc gcagaaatgg acatggatac cccgtgagtt acccggcggg
    12061 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
    12121 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
    12181 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
    12241 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
    12301 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    12361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    12421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    12481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    12541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    12601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    12661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    12721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    12781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    12841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    12901 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    12961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    13021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    13081 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    13141 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    13201 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    13261 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    13321 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    13381 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    13441 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    13501 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    13561 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    13621 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    13681 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    13741 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    13801 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctct
//
