LOCUS       sequence-37849-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2818 (=HL 865 + pHL 489)
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(55..72)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      225..311
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      403..430
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    497..515
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    522..540
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    784..800
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 agactctcaa taaagctagc ttggctgttt tggcggatga gagaagattt tcagcctgat
       61 acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag
      121 cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg
      181 tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
      241 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
      301 gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc
      361 gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg
      421 atggcctttt tgcgtttcta caaactcttt tgtttatttt tctaaataca ttcaaatatg
      481 tatccgctca ggatcctccc tatcagtgat agagattgac atccctatca gtgatagaga
      541 tactccggga acacatcaga tttcctggtg taacgaattt tttaagtgct tcttgcttaa
      601 gcaagtttca tcccgacccc ctcagggtcg ggattttttt attgtgcatt caacgattca
      661 cttcagagac gctaagcttt aatcgtacag ggtagtacaa ataaaaaagg cacgtcagat
      721 gacgtgcctt ttttcttgtg agcagtgaat tcgatccata aatgtgagcg gataacattg
      781 acattgtgag cggataacaa gatactcccg ggtaaggagg aaaaaaaatg gctaaggggc
      841 aatctttaca agatccgttc ctgaacgcac tgcgtc
//
