LOCUS       sequence-378503-       10221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knock-in gene targeting in marmoset cells at SF1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10221)
  AUTHORS   Yoshimatsu S, Kisu I, Qian E, Noce T
  TITLE     A New Horizon in Reproductive Research with Pluripotent Stem Cells: 
            Successful In Vitro Gametogenesis in Rodents, Its Application to 
            Large Animals, and Future In Vitro Reconstitution of Reproductive 
            Organs Such as ""Uteroid"" and ""Oviductoid"".
  JOURNAL   Biology (Basel). 2022 Jun 29;11(7):987. doi: 
            10.3390/biology11070987.
  PUBMED    36101367
REFERENCE   2  (bases 1 to 10221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biology 
            (Basel)."; date: "2022-06-29"; volume: "11(7):987. doi"; pages: " 
            10.3390/biology11070987"
COMMENT     Sequence Label: pKI-cjSF1-tdTomato-PGK-Neo
FEATURES             Location/Qualifiers
     source          1..10221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    80..101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(116..121,122..139,140..146)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    154..170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     159..181
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     178..194
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     178..194
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             806..862
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             863..2293
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    2415..2536
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2452..2471)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2506..2525
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    2576..2609
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2653..3152
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2688..2711)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3085..3104
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3166..3969
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    4297..4330
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5300..5872
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          5949..6014
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(6162..6181)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6227..6282
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6281..6300)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6627..6646)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6628..6646)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6653..6670)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6653..6669)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6662..6684)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6810..7238
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6897..6916)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7107..7128
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             7582..8376
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             join(8394..8462,8463..9254)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8612..8631)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9425..10013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9914..9933
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10167..10184
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
       61 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      121 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
      181 gaaacagcta tgaccatgat tacgccaagc ttcatgattc tcgttcattg tgaccacggt
      241 ggtgatgatg gcagtgatga tggtgacagg tgactgtgga tgggttgttg atggctggca
      301 ctggccacat ttatgaagag cacaggcacg tgggtgtcag tgacgatggt ggtgtttgtg
      361 aaccagtatg acaacaatgg tcatggtgac acggtcccag cagggagacc ctggttgcat
      421 ggttgatggt ggcagtggca agtcttcggt ggtcataggg aaggtggcat tgtgatgccg
      481 gtgacagtgt tggctgccca gccccaaagg ggatggatgg tgacccgatg gtgactgagc
      541 gcctcccttt gcagacgtga agttcctgaa taaccacagc ctggttaaag acgctcagga
      601 gaaggcgaac gctgccctgc tggactacac cctatgccac tacccacact gcggggacaa
      661 gtttcagcag ctgctgctgt gcctagtgga ggtgcgggcc ctgagcatgc aggccaagga
      721 gtacctgtac cacaagcacc tgggcaacga gatgccccgc aacaacctgc tcatcgagat
      781 gctgcaagct aagcagactg ctagcgccac taacttctcc ctgttgaaac aagcagggga
      841 tgtcgaagag aatcccgggc caatggtgag caagggcgag gaggtcatca aagagttcat
      901 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga
      961 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     1021 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
     1081 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
     1141 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
     1201 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
     1261 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
     1321 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca
     1381 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta
     1441 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     1501 acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac
     1561 cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga
     1621 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
     1681 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     1741 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     1801 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     1861 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     1921 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     1981 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     2041 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     2101 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     2161 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     2221 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga
     2281 gctgtacaag taggcggccg cgactctaga tcataatcag ccataccaca tttgtagagg
     2341 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     2401 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     2461 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     2521 tcatcaatgt atcttaaggc gttaggaatt ccgatcatat tcaataaccc ttaatataac
     2581 ttcgtataat gtatgctata cgaagttatt aggtctgaag aggagtttac gtccagccaa
     2641 gctaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     2701 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     2761 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     2821 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     2881 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     2941 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     3001 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     3061 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     3121 tgttctcctc ttcctcatct ccgggccttt cgacctgcag ccaatatggg atcggccatt
     3181 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     3241 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     3301 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     3361 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     3421 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     3481 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     3541 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     3601 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     3661 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     3721 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     3781 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     3841 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     3901 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     3961 ttcttctgag gggatccgct gtaagtctgc agaaattgat gatctattaa acaataaaga
     4021 tgtccactaa aatggaagtt tttcctgtca tactttgtta agaagggtga gaacagagta
     4081 cctacatttt gaatggaagg attggagcta cgggggtggg ggtggggtgg gattagataa
     4141 atgcctgctc tttactgaag gctctttact attgctttat gataatgttt catagttgga
     4201 tatcataatt taaacaagca aaaccaaatt aagggccagc tcattcctcc cactcatgat
     4261 ctatagatcc ctcgatcgag atccggaacc cttaatataa cttcgtataa tgtatgctat
     4321 acgaagttat taggtccctc gtgagcctgg gcagggggcg gggccggggc tgggggcggg
     4381 gccagggcgg ggctgccacc accccaccgg ctccacgtgt ttcattttcc gaggcccacc
     4441 caggagcccc ggccccgtcc ccgtcccaga ggccgctgcc cctgagttct gacacggtgt
     4501 gtttgggaag gtgggtgagg ctgggcaggg cctggcagag gtggggtggc cctggccagc
     4561 caccagcact tgcctgctag ttggagtgcc caaaggaggt ggctggtaac cacccccgcc
     4621 agcccctgcc ctgctcccag atcttcagaa tcgctcctgg attctgaagc ctgcaggtcg
     4681 ggggaggagg ttcgggattc cctagtgggc ctcgatgtcc cttggatcag aggtcatccc
     4741 ttcctcctct cctgaaaaca gacagggaga agttgagcag gtatcaactg ggggagggag
     4801 agggtctcca gtgttcccct atggagaaca ggagggaggt cctctgtttt gtaaactaag
     4861 gataactgag tttgctaaac tgggaggggc tactgggccc tagaggacag caggagaact
     4921 cgttaggaca aagagtcccc ctccctagcc cttctgctcc acctgactct gcaagaggga
     4981 gctttgatca tcatcaggaa agaactttgc tgcagtgtgg ttttcaagag gaagcaaaaa
     5041 gcctctccac ccaggcctgg aatgtttcca cccaatgtcg agcagtgtgg ttttgcaaga
     5101 ggaagcaaaa agcctctcca cccaggcctg gaatgtttcc acccaatgtc gagcaaaccc
     5161 cgcccagcgt cttgtcattg gcgaattcga acacgcagat gcagtcgggg cggcgcggtc
     5221 ccaggtccac ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca
     5281 ggtcctcgcc atggatcctg atgatgttgt tgattcttct aaatcttttg tgatggaaaa
     5341 cttttcttcg taccacggga ctaaacctgg ttatgtagat tccattcaaa aaggtataca
     5401 aaagccaaaa tctggtacac aaggaaatta tgacgatgat tggaaagggt tttatagtac
     5461 cgacaataaa tacgacgctg cgggatactc tgtagataat gaaaacccgc tctctggaaa
     5521 agctggaggc gtggtcaaag tgacgtatcc aggactgacg aaggttctcg cactaaaagt
     5581 ggataatgcc gaaactatta agaaagagtt aggtttaagt ctcactgaac cgttgatgga
     5641 gcaagtcgga acggaagagt ttatcaaaag gttcggtgat ggtgcttcgc gtgtagtgct
     5701 cagccttccc ttcgctgagg ggagttctag cgttgaatat attaataact gggaacaggc
     5761 gaaagcgtta agcgtagaac ttgagattaa ttttgaaacc cgtggaaaac gtggccaaga
     5821 tgcgatgtat gagtatatgg ctcaagcctg tgcaggaaat cgtgtcaggc gatctctttg
     5881 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
     5941 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
     6001 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcag
     6061 atccactaga gtcgaagcgg cttcactcct caggtgcagg ctgcctatca gaaggtggtg
     6121 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     6181 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     6241 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     6301 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     6361 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     6421 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     6481 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     6541 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     6601 tggagatctc tagagggccc aattcgccct atagtgagtc gtattacaat tcactggccg
     6661 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     6721 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6781 aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc
     6841 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6901 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6961 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7021 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7081 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7141 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7201 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaattc agggcgcaag
     7261 ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg
     7321 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag
     7381 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc
     7441 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac
     7501 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag
     7561 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     7621 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     7681 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     7741 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     7801 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     7861 ttgggcgaag tgccggggca ggatctcctg tcatcccacc ttgctcctgc cgagaaagta
     7921 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     7981 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     8041 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     8101 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     8161 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     8221 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     8281 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     8341 atcgccttct atcgccttct tgacgagttc ttctgaattg aaaaaggaag agtatgagta
     8401 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8461 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     8521 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8581 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     8641 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8701 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8761 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8821 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8881 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8941 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9001 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9061 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9121 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9181 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9241 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9301 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9361 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9421 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9481 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9541 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     9601 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9661 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9721 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9781 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9841 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9901 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9961 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10021 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10081 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    10141 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    10201 caatacgcaa accgcctctc c
//
