LOCUS       sequence-378555-        8729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral transduction of eGFP gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Lee WS, Lavery L, Rousseaux MWC, Rutledge EB, Jang Y, Wan YW, Wu SR,
            Kim W, Al-Ramahi I, Rath S, Adamski CJ, Bondar VV, Tewari A, 
            Soleimani S, Mota S, Yalamanchili HK, Orr HT, Liu Z, Botas J, Zoghbi
            HY
  TITLE     Dual targeting of brain region-specific kinases potentiates 
            neurological rescue in Spinocerebellar ataxia type 1.
  JOURNAL   EMBO J. 2021 Apr 1;40(7):e106106. doi: 10.15252/embj.2020106106. 
            Epub 2021 Mar 11.
  PUBMED    33709453
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-04-1"; volume: "40(7):e106106. doi"; pages: " 
            10.15252/embj.2020106106. Epub 2021 Mar 11"
COMMENT     Sequence Label: W118-1_eGFP
FEATURES             Location/Qualifiers
     source          1..8729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     276..293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    410..431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(446..451,452..469,470..476)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    484..500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     489..511
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     508..524
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     508..524
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     543..563
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        545..563
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        591..817
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             818..998
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1045..1170
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1663..1896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2081..2125
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2392..2508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2531..2834
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2835..3038
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2989..3009
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3035..3059
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3143..3167
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3188..3190,3191..3193,3194..3907)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3233..3254)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3494..3513)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3841..3862
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3924..3948)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3979..4567
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4032..4052)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4450..4461)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4581..5080
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4616..4639)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5013..5032
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5101..5700
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5101..5120)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5597..5617
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5838..6071
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6143..6264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6180..6199)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6234..6253
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6304..6439
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6459..6478)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6460..6478)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6488..6505)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6488..6504)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6497..6519)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6646..7101
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6733..6752)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6943..6964
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7127..7231
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7232..7300,7301..8092)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7450..7469)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8263..8729,1..122)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      421 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      481 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      541 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
      601 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
      661 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
      721 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
      781 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
      841 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      901 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      961 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1021 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1081 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1141 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1201 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1261 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1321 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1381 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1441 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1501 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1561 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1621 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1681 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1741 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1801 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1861 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1921 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1981 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2041 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2101 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2161 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2221 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2281 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2341 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     2401 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2461 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt
     2521 gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2581 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2641 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2701 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2761 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2821 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2881 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2941 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3001 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     3061 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg
     3121 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctccg cggccgcccc
     3181 cttcaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3241 gctggacggc gacgtaaacg gccacaagtt cagcgtgtct ggcgagggcg agggcgatgc
     3301 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     3361 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     3421 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3481 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3541 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3601 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3661 gaagaacggc atcaaggcga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3721 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3781 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3841 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3901 caagtaaaag ggtgggcgcg ccgacccagc tttcttgtac aaagtggttg atatccagca
     3961 cagtggcggc cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4021 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4081 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4141 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4201 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4261 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4321 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     4381 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4441 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4501 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4561 tccccgcctg gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     4621 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     4681 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     4741 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     4801 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     4861 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     4921 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     4981 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     5041 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc
     5101 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     5161 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     5221 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     5281 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     5341 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     5401 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     5461 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     5521 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     5581 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     5641 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     5701 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatctcga
     5761 gggcccggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5821 aaaagaaaag gggggactgg aagggctagc tcactcccaa cgaagacaag atctgctttt
     5881 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5941 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6001 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6061 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     6121 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     6181 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6241 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     6301 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6361 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     6421 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     6481 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     6541 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     6601 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     6661 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6721 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6781 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6841 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     6901 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6961 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7021 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     7081 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7141 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7201 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7261 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7321 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     7381 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     7441 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     7501 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     7561 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     7621 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     7681 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     7741 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     7801 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     7861 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     7921 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     7981 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8041 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8101 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8161 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8221 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8281 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     8341 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8401 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8461 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8521 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8581 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8641 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8701 tccggtaagc ggcagggtcg gaacaggag
//
