LOCUS       sequence-37856-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2839 (=HL 2752 + pHL 720)
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    218..234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        292..396
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(615..634)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgcatgctcg cggtaccttt aatcaggttg ttcaggttag tgacataagt ctgatggtgc
       61 ttgccgtagt gatactcgat ggtttccgca gaaatgtgcg gtgccagagc atctttagca
      121 tatggtagtg caggtaattc gaatgacatt gctactctcc tttattatta atttgcatac
      181 gtacaatagc cttattgtgc gtatggtcga cagtatcttg ttatccgctc acaatgtcaa
      241 tgttatccgc tcacatttat gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
      301 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
      361 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
      421 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
      481 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
      541 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
      601 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
      661 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
      721 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
      781 gataacactg cggccaactt acttctgaca acg
//
