LOCUS       sequence-378570-        8049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression vector for NGF-EGFR-FRB-GluGlu construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pBabe NGF-EGFR-FRB-GluGlu neo
FEATURES             Location/Qualifiers
     source          1..8049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3853..4131
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             4138..4155
                     /codon_start=1
                     /product="epitope tag from middle T antigen of mouse 
                     polyoma virus"
                     /label=Glu-Glu tag
                     /translation="EYMPME"
     CDS             4156..4173
                     /codon_start=1
                     /product="epitope tag from middle T antigen of mouse 
                     polyoma virus"
                     /label=Glu-Glu tag
                     /translation="EYMPME"
     primer_bind     complement(4188..4208)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4193..4522
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4373..4508
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4879..5673
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4933..4952)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5543..5562
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6196..6215
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             6477..6946
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(7061..7852,7853..7921))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7922..8026)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccca gtgtggtggt
     1381 acgtaggaat tgatgggggc aggtgccacc ggccgcgcca tggacgggcc gcgcctgctg
     1441 ctgttgctgc ttctgggggt gtcccttgga ggtgccaagg aggcatgccc cacaggcctg
     1501 tacacacaca gcggtgagtg ctgcaaagcc tgcaacctgg gcgagggtgt ggcccagccc
     1561 tgtggagcca accagaccgt gtgtgagccc tgcctggaca gcgtgacgtt ctccgacgtg
     1621 gtgagcgcga ccgagccgtg caagccgtgc accgagtgcg tggggctcca gagcatgtcg
     1681 gcgccgtgcg tggaggccga cgacgccgtg tgccgctgcg cctacggcta ctaccaggat
     1741 gagacgactg ggcgctgcga ggcgtgccgc gtgtgcgagg cgggctcggg cctcgtgttc
     1801 tcctgccagg acaagcagaa caccgtgtgc gaggagtgcc ccgacggcac gtattccgac
     1861 gaggccaacc acgtggaccc gtgcctgccc tgcaccgtgt gcgaggacac cgagcgccag
     1921 ctccgcgagt gcacacgctg ggccgacgcc gagtgcgagg agatccctgg ccgttggatt
     1981 acacggtcca cacccccaga gggctcggac agcacagccc ccagcaccca ggagcctgag
     2041 gcacctccag aacaagacct catagccagc acggtggcag gtgtggtgac cacagtgatg
     2101 ggcagctccc agcccgtggt gacccgaggc accaccgaca acctcatccc tgtctattgc
     2161 tccatcctgg ctgctgtggt tgtgggtctt gtggcctaca tagccttcaa gaggactaga
     2221 cgaaggcgcc acatcgttcg gaagcgcacg ctgcggaggc tgctgcagga gagggagctt
     2281 gtggagcctc ttacacccag tggagaagct cccaaccaag ctctcttgag gatcttgaag
     2341 gaaactgaat tcaaaaagat caaagtgctg ggctccggtg cgttcggcac ggtgtataag
     2401 ggactctgga tcccagaagg tgagaaagtt aaaattcccg tcgctatcaa ggaattaaga
     2461 gaagcaacat ctccgaaagc caacaaggaa atcctcgatg aagcctacgt gatggccagc
     2521 gtggacaacc cccacgtgtg ccgcctgctg ggcatctgcc tcacctccac cgtgcagctc
     2581 atcacgcagc tcatgtcctt cggctgcctc ctggactatg tccgggaaca caaagacaat
     2641 attggctccc agtacctgct caactggtgt gtgcagatcg caaagggcat gaactacttg
     2701 gaggaccgtc gcttggtgca ccgcgacctg gcagccagga acgtactggt gaaaacaccg
     2761 cagcatgtca agatcacaga ttttgggctg gccaaactgc tgggtgcgga agagaaagaa
     2821 taccatgcag aaggaggcaa agtgcctatc aagtggatgg cattggaatc aattttacac
     2881 agaatctata cccaccagag tgatgtctgg agctacgggg tgaccgtttg ggagttgatg
     2941 acctttggat ccaagccata tgacggaatc cctgccagcg agatctcctc catcctggag
     3001 aaaggagaac gcctccctca gccacccata tgtaccatcg atgtctacat gatcatggtc
     3061 aagtgctgga tgatagacgc agatagtcgc ccaaagttcc gtgagttgat catcgaattc
     3121 tccaaaatgg cccgagaccc ccagcgctac cttgtcattc agggggatga aagaatgcat
     3181 ttgccaagtc ctacagactc caacttctac cgtgccctga tggatgaaga agacatggac
     3241 gacgtggtgg atgccgacga gtacctcatc ccacagcagg gcttcttcag cagcccctcc
     3301 acgtcacgga ctcccctcct gagctctctg agtgcaacca gcaacaattc caccgtggct
     3361 tgcattgata gaaatgggct gcaaagctgt cccatcaagg aagacagctt cttgcagcga
     3421 tacagctcag accccacagg cgccttgact gaggacagca tagacgacac cttcctccca
     3481 gtgcctgaat acataaacca gtccgttccc aaaaggcccg ctggctctgt gcagaatcct
     3541 gtctatcaca atcagcctct gaaccccgcg cccagcagag acccacacta ccaggacccc
     3601 cacagcactg cagtgggcaa ccccgagtat ctcaacactg tccagcccac ctgtgtcaac
     3661 agcacattcg acagccctgc ccactgggcc cagaaaggca gccaccaaat tagcctggac
     3721 aaccctgact accagcagga cttctttccc aaggaagcca agccaaatgg catctttaag
     3781 ggctccacag ctgaaaatgc agaataccta agggtcgcgc cacaaagcag tgaatttatt
     3841 ggagcaacta gtatcctctg gcatgagatg tggcatgaag gcctggaaga ggcatctcgt
     3901 ttgtactttg gggaaaggaa cgtgaaaggc atgtttgagg tgctggagcc cttgcatgct
     3961 atgatggaac ggggccccca gactctgaag gaaacatcct ttaatcaggc ctatggtcga
     4021 gatttaatgg aggcccaaga gtggtgcagg aagtacatga aatcagggaa tgtcaaggac
     4081 ctcctccaag cctgggacct ctattatcat gtgttccgac gaatctcaaa gtctagtgag
     4141 tatatgccca tggaggagta catgccgatg gagtaagtcg accctgtgga atgtgtgtca
     4201 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4261 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4321 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4381 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4441 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     4501 tggaggccta ggcttttgca aaaagcttca cgctgccgca agcactcagg gcgcaagggc
     4561 tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg accccggatg
     4621 aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta
     4681 gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa
     4741 ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
     4801 atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca
     4861 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     4921 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     4981 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     5041 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     5101 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     5161 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     5221 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     5281 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     5341 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     5401 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     5461 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5521 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     5581 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     5641 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gcatcgatgc
     5701 atcgatgcgg cgggtgtggt ggttacgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5761 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5821 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     5881 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5941 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6001 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6061 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6121 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6181 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6241 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6301 cagcaacgca tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
     6361 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
     6421 aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga
     6481 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
     6541 agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
     6601 ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa
     6661 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
     6721 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
     6781 gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc
     6841 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
     6901 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg cagcatgtat
     6961 caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag tgcggccgca
     7021 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7081 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7141 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7201 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7261 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7321 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7381 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7441 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7501 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7561 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7621 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7681 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7741 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7801 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7861 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7921 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7981 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8041 aagtgccac
//
