LOCUS       sequence-378596-       12060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of AMPA GluA1 with N terminal super ecliptic phluorin 
            (SEP) fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12060)
  AUTHORS   Kim D, Park P, Li X, Wong Campos JD, Tian H, Moult EM, Grimm JB, 
            Lavis L, Cohen AE
  TITLE     Mapping memories: pulse-chase labeling reveals AMPA receptor 
            dynamics during memory formation.
  JOURNAL   bioRxiv. 2023 May 29:2023.05.26.541296. doi: 
            10.1101/2023.05.26.541296. Preprint.
  PUBMED    37292614
REFERENCE   2  (bases 1 to 12060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 May 29:2023.05.26.541296. doi: 10.1101/2023.05.26.541296. 
            Preprint."
COMMENT     Sequence Label: TL021_lenti_CMV:SEP-GluA1
FEATURES             Location/Qualifiers
     source          1..12060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..30
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(149..169)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        154..483
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      334..469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     396..415
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        531..578
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             597..971
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1101..1222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1138..1157)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1192..1211
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1271..1287)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1271..1287)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1284..1306)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1295..1311
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1319..1325,1326..1343,1344..1349))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1364..1385
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1502..1519)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1673..2261)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1753..1772)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2432..3223,3224..3292))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3055..3074
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3293..3397)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3472..3491)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3663..4042
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4044..4242
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             4260..4440
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4487..4612
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5105..5338
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5523..5567
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5865..5982
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6041..6344
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6345..6548
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6545..6569
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6736..7446
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(6763..6791)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7386..7406
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    10177..10765
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10230..10250)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10648..10659)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10768..10784)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10769..10785)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11290..11470
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11496..11513)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11502..11726
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(11772..12060,1..140)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11859..11878)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
       61 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      121 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
      181 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
      241 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      301 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
      361 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
      421 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
      481 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
      541 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
      601 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
      661 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
      721 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
      781 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
      841 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
      901 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
      961 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     1021 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     1081 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     1141 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     1201 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     1261 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1321 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1381 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     1441 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1501 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1561 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1621 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1681 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1741 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1801 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1861 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1921 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1981 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2041 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2101 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2161 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2221 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2281 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2341 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2401 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2461 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2521 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2581 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2641 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2701 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2761 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2821 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2881 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2941 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3001 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3061 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3121 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3181 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3241 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3301 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3361 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3421 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
     3481 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     3541 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     3601 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
     3661 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     3721 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     3781 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     3841 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     3901 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     3961 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     4021 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     4081 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     4141 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     4201 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg
     4261 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4321 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4381 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4441 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     4501 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     4561 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     4621 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     4681 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     4741 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     4801 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     4861 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     4921 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     4981 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     5041 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     5101 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     5161 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     5221 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     5281 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     5341 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     5401 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     5461 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     5521 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     5581 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     5641 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     5701 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     5761 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     5821 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     5881 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     5941 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     6001 agcagagatc cagtttggtt aattaagctt gggagttccg cgttacataa cttacggtaa
     6061 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     6121 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag catttacggt
     6181 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     6241 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     6301 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     6361 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     6421 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     6481 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     6541 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     6601 tccatagaag acaccgactc tagaggatcc accatgccgt acatctttgc ctttttctgc
     6661 accggttttc taggtgcggt tgtgggtgcc aatgcgcgag gtggaggcgg tgggggaggg
     6721 ggtggaggaa cgcgtagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     6781 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     6841 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcct
     6901 tggccaacac ttgtcactac tttaacttat ggtgttcaat gcttttcaag atacccagat
     6961 catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     7021 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     7081 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     7141 cttggacaca aattggaata caactataac gatcaccagg tgtacatcat ggcagacaaa
     7201 caaaagaatg gaatcaaagc taacttcaaa attagacaca acattgaaga tggaggcgtt
     7261 caactagcag accattatca acaaaatact ccaattggcg atgggcccgt ccttttacca
     7321 gacaaccatt acctgtttac aacttctact ctttcgaaag atcccaacga aaagagagac
     7381 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     7441 tacaaaacgc gtggaggtgg agggggcgga ggtggcgggg gttcgcgctt ccccaacaat
     7501 atccagatag gggggttatt tccaaaccaa caatcacagg aacatgcggc ttttaggttt
     7561 gctttgtcac aactcacgga gccccccaag ctgcttcccc agatcgatat tgtgaacatc
     7621 agcgacagct ttgagatgac ttaccgtttc tgttcccagt tctccaaagg agtctatgcc
     7681 atctttggat tttatgaacg aaggactgtc aacatgctga cctccttctg tggggccctc
     7741 catgtgtgct tcattactcc aagttttcct gttgacacat ccaatcaatt tgtccttcag
     7801 ctacgcccgg aactacagga agctctcatt agcattatcg accattacaa gtggcaaacc
     7861 tttgtctaca tttatgatgc tgaccggggc ctgtcagtcc tgcagagagt cttggataca
     7921 gccgcagaga agaactggca ggtaacggct gtcaacattc tgacaaccac cgaggaagga
     7981 taccggatgc tctttcagga cctggagaag aaaaaggaga ggctggtggt ggttgactgt
     8041 gaatcagaac gcctcaacgc catcctgggc cagatcgtga agctagaaaa gaatggcatc
     8101 gggtaccact acatcctcgc caatctgggc ttcatggaca ttgacttaaa taagttcaag
     8161 gagagcggag ccaatgtgac aggtttccag ctggtgaact acacagacac gatcccagcc
     8221 agaatcatgc agcaatggag gacaagtgac tcccgagacc ataccagggt ggactggaag
     8281 aggccaaagt acacttctgc tctcacctat gatggtgtca aggtgatggc tgaggccttc
     8341 caaagcctgc ggaggcagag gattgacata tcccgccggg ggaatgctgg ggactgtctg
     8401 gctaacccag ctgtgccctg gggtcaaggg atcgacatcc agagagccct gcagcaggtg
     8461 cgcttcgaag gtttgacagg aaatgtgcag ttcaacgaga aagggcgccg gaccaattac
     8521 accctccacg tgatcgaaat gaaacatgat ggaatccgaa agattggtta ctggaatgaa
     8581 gacgataaat ttgtccccgc agccaccgac gctcaggctg gaggggacaa ctcaagcgtc
     8641 cagaatagga cctacatcgt cactactatc ctcgaagatc cttacgtgat gcttaaaaag
     8701 aatgccaacc agtttgaggg caatgaccgc tatgagggct actgtgtgga gctggctgca
     8761 gagatcgcca agcacgtggg ctactcctac cgacttgaga ttgtcagcga cggcaaatat
     8821 ggagcccggg atcccgacac aaaggcttgg aatggcatgg tgggagaact ggtctatgga
     8881 agagcagacg tggctgtggc tcccttgacc ataaccttgg tccgggagga agtcatcgac
     8941 ttctccaagc cattcatgag tttgggaatc tccattatga ttaagaagcc acagaagtcc
     9001 aagccaggtg tcttctcctt tcttgaccct ttggcctatg agatctggat gtgtatagtg
     9061 tttgcctaca ttggagtgag cgtcgtcctc ttcctggtca gccgtttcag cccctacgaa
     9121 tggcacagcg aagagtttga agagggacga gaccagacaa ccagtgacca gtcaaatgag
     9181 tttggcatat tcaacagcct gtggttctcc ctgggggcct tcatgcagca aggatgtgac
     9241 atttccccca ggtccctgtc cggacgcatc gtcggcggcg tctggtggtt cttcactttg
     9301 atcatcatct cctcgtacac agccaacctg gctgccttcc tgactgtgga gaggatggtg
     9361 tctcccattg agagtgcaga ggacctggca aagcagacgg aaattgctta tgggacattg
     9421 gaagcaggct ccactaagga gttcttcagg agatctaaaa tcgctgtgtt tgagaagatg
     9481 tggacataca tgaagtctgc agaaccatcc gtgtttgttc ggaccacaga ggaaggcatg
     9541 atcagagtga gaaaatctaa aggcaaatac gcctacctcc tggagtccac catgaatgag
     9601 tatattgagc aacgaaagcc ctgtgacacc atgaaagtgg gaggtaactt ggattccaaa
     9661 ggctatggca ttgcgacacc caaggggtcc gccctgagaa atccagtaaa cctggcagtg
     9721 ttaaaactga acgagcaggg gcttttggac aaattgaaaa acaaatggtg gtacgacaag
     9781 ggcgagtgcg gcagcggggg aggtgactcc aaggacaaga ccagcgcttt gagcctcagc
     9841 aatgtggcag gcgtgttcta catcctgatt ggagggctgg gactggccat gctggttgcc
     9901 ttaatcgagt tctgctacaa atcccgtagc gagtcgaagc ggatgaaggg tttctgtttg
     9961 atcccacagc aatccatcaa tgaagccata cggacatcga ccctcccccg gaacagtggg
    10021 gcaggagcca gcggaggagg cggcagtgga gagaatggcc gggtggtcag ccaggacttc
    10081 cccaagtcca tgcaatccat tccctgcatg agtcacagtt cagggatgcc cttgggagcc
    10141 acaggattgt aagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
    10201 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
    10261 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
    10321 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
    10381 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
    10441 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
    10501 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
    10561 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
    10621 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
    10681 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
    10741 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat
    10801 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
    10861 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
    10921 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
    10981 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
    11041 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
    11101 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
    11161 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
    11221 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
    11281 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
    11341 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
    11401 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
    11461 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
    11521 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
    11581 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
    11641 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
    11701 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
    11761 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
    11821 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
    11881 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
    11941 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
    12001 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
//
