LOCUS       sequence-378598-       12033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of AMPA GluA1 with N terminal super ecliptic phluorin 
            (SEP) fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12033)
  AUTHORS   Kim D, Park P, Li X, Wong Campos JD, Tian H, Moult EM, Grimm JB, 
            Lavis L, Cohen AE
  TITLE     Mapping memories: pulse-chase labeling reveals AMPA receptor 
            dynamics during memory formation.
  JOURNAL   bioRxiv. 2023 May 29:2023.05.26.541296. doi: 
            10.1101/2023.05.26.541296. Preprint.
  PUBMED    37292614
REFERENCE   2  (bases 1 to 12033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 May 29:2023.05.26.541296. doi: 10.1101/2023.05.26.541296. 
            Preprint."
COMMENT     Sequence Label: TL022_lenti_hSyn:SEP-GluA1
FEATURES             Location/Qualifiers
     source          1..12033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(134..153)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        325..704
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        706..904
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     859..879
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             922..1102
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1149..1274
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1767..2000
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2185..2229
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2527..2644
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2719..3166
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             3327..4037
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(3354..3382)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3977..3997
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    6812..7400
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6865..6885)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7283..7294)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7403..7419)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7404..7420)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7925..8105
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8131..8148)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8137..8361
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8407..8835
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8494..8513)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8704..8725
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8844..8864)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8849..9178
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9029..9164
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9091..9110
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9226..9273
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9292..9666
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9796..9917
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9833..9852)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9887..9906
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9966..9982)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9966..9982)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9979..10001)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9990..10006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10014..10020,10021..10038,10039..10044))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10059..10080
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10197..10214)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10368..10956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10448..10467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11127..11918,11919..11987))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11750..11769
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(11988..12033,1..59))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
       61 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
      121 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
      181 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
      241 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
      301 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
      361 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      421 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      481 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      541 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      601 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      661 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      721 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      781 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      841 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      901 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
      961 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     1021 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     1081 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     1141 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     1201 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     1261 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     1321 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     1381 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     1441 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     1501 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     1561 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     1621 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     1681 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     1741 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     1801 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     1861 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     1921 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     1981 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     2041 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     2101 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     2161 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     2221 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     2281 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     2341 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     2401 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     2461 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
     2521 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2581 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2641 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaagt gtctagactg
     2701 cagagggccc tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg
     2761 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
     2821 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
     2881 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
     2941 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
     3001 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
     3061 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
     3121 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga gaaggatcca
     3181 caacttcgta tatacctttc tatacgaagt tgtggcgcgc caccatgccg tacatctttg
     3241 cctttttctg caccggtttt ctaggtgcgg ttgtgggtgc caatgcgcga ggtggaggcg
     3301 gtgggggagg gggtggagga acgcgtagta aaggagaaga acttttcact ggagttgtcc
     3361 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     3421 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     3481 tacctgttcc ttggccaaca cttgtcacta ctttaactta tggtgttcaa tgcttttcaa
     3541 gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg
     3601 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
     3661 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
     3721 atggaaacat tcttggacac aaattggaat acaactataa cgatcaccag gtgtacatca
     3781 tggcagacaa acaaaagaat ggaatcaaag ctaacttcaa aattagacac aacattgaag
     3841 atggaggcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatgggcccg
     3901 tccttttacc agacaaccat tacctgttta caacttctac tctttcgaaa gatcccaacg
     3961 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     4021 tggatgaact atacaaaacg cgtggaggtg gagggggcgg aggtggcggg ggttcgcgct
     4081 tccccaacaa tatccagata ggggggttat ttccaaacca acaatcacag gaacatgcgg
     4141 cttttaggtt tgctttgtca caactcacgg agccccccaa gctgcttccc cagatcgata
     4201 ttgtgaacat cagcgacagc tttgagatga cttaccgttt ctgttcccag ttctccaaag
     4261 gagtctatgc catctttgga ttttatgaac gaaggactgt caacatgctg acctccttct
     4321 gtggggccct ccatgtgtgc ttcattactc caagttttcc tgttgacaca tccaatcaat
     4381 ttgtccttca gctacgcccg gaactacagg aagctctcat tagcattatc gaccattaca
     4441 agtggcaaac ctttgtctac atttatgatg ctgaccgggg cctgtcagtc ctgcagagag
     4501 tcttggatac agccgcagag aagaactggc aggtaacggc tgtcaacatt ctgacaacca
     4561 ccgaggaagg ataccggatg ctctttcagg acctggagaa gaaaaaggag aggctggtgg
     4621 tggttgactg tgaatcagaa cgcctcaacg ccatcctggg ccagatcgtg aagctagaaa
     4681 agaatggcat cgggtaccac tacatcctcg ccaatctggg cttcatggac attgacttaa
     4741 ataagttcaa ggagagcgga gccaatgtga caggtttcca gctggtgaac tacacagaca
     4801 cgatcccagc cagaatcatg cagcaatgga ggacaagtga ctcccgagac cataccaggg
     4861 tggactggaa gaggccaaag tacacttctg ctctcaccta tgatggtgtc aaggtgatgg
     4921 ctgaggcctt ccaaagcctg cggaggcaga ggattgacat atcccgccgg gggaatgctg
     4981 gggactgtct ggctaaccca gctgtgccct ggggtcaagg gatcgacatc cagagagccc
     5041 tgcagcaggt gcgcttcgaa ggtttgacag gaaatgtgca gttcaacgag aaagggcgcc
     5101 ggaccaatta caccctccac gtgatcgaaa tgaaacatga tggaatccga aagattggtt
     5161 actggaatga agacgataaa tttgtccccg cagccaccga cgctcaggct ggaggggaca
     5221 actcaagcgt ccagaatagg acctacatcg tcactactat cctcgaagat ccttacgtga
     5281 tgcttaaaaa gaatgccaac cagtttgagg gcaatgaccg ctatgagggc tactgtgtgg
     5341 agctggctgc agagatcgcc aagcacgtgg gctactccta ccgacttgag attgtcagcg
     5401 acggcaaata tggagcccgg gatcccgaca caaaggcttg gaatggcatg gtgggagaac
     5461 tggtctatgg aagagcagac gtggctgtgg ctcccttgac cataaccttg gtccgggagg
     5521 aagtcatcga cttctccaag ccattcatga gtttgggaat ctccattatg attaagaagc
     5581 cacagaagtc caagccaggt gtcttctcct ttcttgaccc tttggcctat gagatctgga
     5641 tgtgtatagt gtttgcctac attggagtga gcgtcgtcct cttcctggtc agccgtttca
     5701 gcccctacga atggcacagc gaagagtttg aagagggacg agaccagaca accagtgacc
     5761 agtcaaatga gtttggcata ttcaacagcc tgtggttctc cctgggggcc ttcatgcagc
     5821 aaggatgtga catttccccc aggtccctgt ccggacgcat cgtcggcggc gtctggtggt
     5881 tcttcacttt gatcatcatc tcctcgtaca cagccaacct ggctgccttc ctgactgtgg
     5941 agaggatggt gtctcccatt gagagtgcag aggacctggc aaagcagacg gaaattgctt
     6001 atgggacatt ggaagcaggc tccactaagg agttcttcag gagatctaaa atcgctgtgt
     6061 ttgagaagat gtggacatac atgaagtctg cagaaccatc cgtgtttgtt cggaccacag
     6121 aggaaggcat gatcagagtg agaaaatcta aaggcaaata cgcctacctc ctggagtcca
     6181 ccatgaatga gtatattgag caacgaaagc cctgtgacac catgaaagtg ggaggtaact
     6241 tggattccaa aggctatggc attgcgacac ccaaggggtc cgccctgaga aatccagtaa
     6301 acctggcagt gttaaaactg aacgagcagg ggcttttgga caaattgaaa aacaaatggt
     6361 ggtacgacaa gggcgagtgc ggcagcgggg gaggtgactc caaggacaag accagcgctt
     6421 tgagcctcag caatgtggca ggcgtgttct acatcctgat tggagggctg ggactggcca
     6481 tgctggttgc cttaatcgag ttctgctaca aatcccgtag cgagtcgaag cggatgaagg
     6541 gtttctgttt gatcccacag caatccatca atgaagccat acggacatcg accctccccc
     6601 ggaacagtgg ggcaggagcc agcggaggag gcggcagtgg agagaatggc cgggtggtca
     6661 gccaggactt ccccaagtcc atgcaatcca ttccctgcat gagtcacagt tcagggatgc
     6721 ccttgggagc cacaggattg taagctagct aaacaacttc gtatatacct ttctatacga
     6781 agttgtcgaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     6841 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6901 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6961 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     7021 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     7081 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     7141 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     7201 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     7261 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     7321 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     7381 cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc
     7441 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     7501 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     7561 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7621 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     7681 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     7741 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     7801 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     7861 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     7921 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7981 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8041 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8101 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     8161 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     8221 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     8281 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     8341 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     8401 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     8461 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     8521 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     8581 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     8641 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     8701 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     8761 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     8821 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     8881 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     8941 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9001 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     9061 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     9121 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     9181 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     9241 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     9301 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     9361 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     9421 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     9481 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     9541 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     9601 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     9661 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     9721 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     9781 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9841 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9901 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     9961 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    10021 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    10081 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    10141 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    10201 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    10261 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    10321 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    10381 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    10441 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    10501 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    10561 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    10621 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    10681 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    10741 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    10801 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    10861 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    10921 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    10981 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    11041 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    11101 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    11161 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    11221 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    11281 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    11341 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    11401 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    11461 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    11521 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    11581 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    11641 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    11701 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    11761 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    11821 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    11881 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    11941 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    12001 caatattatt gaagcattta tcagggttat tgt
//
