LOCUS       sequence-378599-       12216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of AMPA GluA1 with N terminal HaloTag fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12216)
  AUTHORS   Kim D, Park P, Li X, Wong Campos JD, Tian H, Moult EM, Grimm JB, 
            Lavis L, Cohen AE
  TITLE     Mapping memories: pulse-chase labeling reveals AMPA receptor 
            dynamics during memory formation.
  JOURNAL   bioRxiv. 2023 May 29:2023.05.26.541296. doi: 
            10.1101/2023.05.26.541296. Preprint.
  PUBMED    37292614
REFERENCE   2  (bases 1 to 12216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 May 29:2023.05.26.541296. doi: 10.1101/2023.05.26.541296. 
            Preprint."
COMMENT     Sequence Label: TL024_lenti_hSyn:HaloTag-GluA1
FEATURES             Location/Qualifiers
     source          1..12216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     67..88
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(207..227)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        212..541
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      392..527
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     454..473
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        589..636
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             655..1029
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1159..1280
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1196..1215)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1250..1269
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1329..1345)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1329..1345)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1342..1364)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1353..1369
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1377..1383,1384..1401,1402..1407))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1422..1443
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1560..1577)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1731..2319)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1811..1830)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2490..3281,3282..3350))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3113..3132
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3351..3455)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3530..3549)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3721..4100
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4102..4300
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4255..4275
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4318..4498
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4545..4670
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5163..5396
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5581..5625
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5923..6040
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6115..6562
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    10391..10979
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10444..10464)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10862..10873)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10982..10998)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10983..10999)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11504..11684
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11710..11727)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11716..11940
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(11986..12216,1..198)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12073..12092)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
       61 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
      121 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
      181 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
      241 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
      301 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
      361 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
      421 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
      481 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
      541 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
      601 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
      661 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
      721 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
      781 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
      841 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
      901 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
      961 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     1021 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     1081 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     1141 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     1201 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     1261 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     1321 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     1381 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     1441 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     1501 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     1561 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1621 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1681 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1741 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1801 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1861 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1921 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1981 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2041 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2101 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2161 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2221 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2281 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2341 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2401 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2461 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2521 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2581 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2641 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2701 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2761 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     2821 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2881 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2941 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3001 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3061 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3121 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3181 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3241 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3301 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3361 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3421 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3481 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     3541 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     3601 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     3661 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     3721 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     3781 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     3841 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     3901 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     3961 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     4021 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     4081 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     4141 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     4201 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     4261 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     4321 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     4381 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     4441 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     4501 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     4561 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     4621 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     4681 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     4741 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     4801 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     4861 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     4921 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     4981 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     5041 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     5101 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     5161 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     5221 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     5281 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     5341 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     5401 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     5461 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     5521 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     5581 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     5641 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     5701 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     5761 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     5821 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     5881 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     5941 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     6001 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     6061 cagagatcca gtttggttaa ttaagtgtct agactgcaga gggccctgcg tatgagtgca
     6121 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
     6181 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
     6241 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
     6301 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
     6361 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
     6421 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
     6481 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
     6541 cgtgtcgtgc ctgagagcgc agtcgagaag gatccacaac ttcgtatata cctttctata
     6601 cgaagttgtg gcgcgccacc atgccgtaca tctttgcctt tttctgcacc ggttttctag
     6661 gtgcggttgt gggtgccaat gcgcgaggtg gaggcggtgg gggagggggt ggaggaacgc
     6721 gtatcggtac tggctttcca ttcgaccccc attatgtgga agtcctgggc gagcgcatgc
     6781 actacgtcga tgttggtccg cgcgatggca cccctgtgct gttcctgcac ggtaacccga
     6841 cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc accgacccat cgctgcattg
     6901 ctccagacct gatcggtatg ggcaaatccg acaaaccaga cctgggttat ttcttcgacg
     6961 accacgtccg cttcatggat gccttcatcg aagccctggg tctggaagag gtcgtcctgg
     7021 tcattcacga ctggggctcc gctctgggtt tccactgggc caagcgcaat ccagagcgcg
     7081 tcaaaggtat tgcatttatg gagttcatcc gccctatccc gacctgggac gaatggccag
     7141 aatttgcccg cgagaccttc caggccttcc gcaccaccga cgtcggccgc aagctgatca
     7201 tcgatcagaa cgtttttatc gagggtacgc tgccgatggg tgtcgtccgc ccgctgactg
     7261 aagtcgagat ggaccattac cgcgagccgt tcctgaatcc tgttgaccgc gagccactgt
     7321 ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc gaacatcgtc gcgctggtcg
     7381 aagaatacat ggactggctg caccagtccc ctgtcccgaa gctgctgttc tggggcaccc
     7441 caggcgttct gatcccaccg gccgaagccg ctcgcctggc caaaagcctg cctaactgca
     7501 aggctgtgga catcggcccg ggtctgaatc tgctgcaaga agacaacccg gacctgatcg
     7561 gcagcgagat cgcgcgctgg ctgtcgacgc tcgagatttc cggcgagcca accactacgc
     7621 gtggaggtgg agggggcgga ggtggcgggg gttcgcgctt ccccaacaat atccagatag
     7681 gggggttatt tccaaaccaa caatcacagg aacatgcggc ttttaggttt gctttgtcac
     7741 aactcacgga gccccccaag ctgcttcccc agatcgatat tgtgaacatc agcgacagct
     7801 ttgagatgac ttaccgtttc tgttcccagt tctccaaagg agtctatgcc atctttggat
     7861 tttatgaacg aaggactgtc aacatgctga cctccttctg tggggccctc catgtgtgct
     7921 tcattactcc aagttttcct gttgacacat ccaatcaatt tgtccttcag ctacgcccgg
     7981 aactacagga agctctcatt agcattatcg accattacaa gtggcaaacc tttgtctaca
     8041 tttatgatgc tgaccggggc ctgtcagtcc tgcagagagt cttggataca gccgcagaga
     8101 agaactggca ggtaacggct gtcaacattc tgacaaccac cgaggaagga taccggatgc
     8161 tctttcagga cctggagaag aaaaaggaga ggctggtggt ggttgactgt gaatcagaac
     8221 gcctcaacgc catcctgggc cagatcgtga agctagaaaa gaatggcatc gggtaccact
     8281 acatcctcgc caatctgggc ttcatggaca ttgacttaaa taagttcaag gagagcggag
     8341 ccaatgtgac aggtttccag ctggtgaact acacagacac gatcccagcc agaatcatgc
     8401 agcaatggag gacaagtgac tcccgagacc ataccagggt ggactggaag aggccaaagt
     8461 acacttctgc tctcacctat gatggtgtca aggtgatggc tgaggccttc caaagcctgc
     8521 ggaggcagag gattgacata tcccgccggg ggaatgctgg ggactgtctg gctaacccag
     8581 ctgtgccctg gggtcaaggg atcgacatcc agagagccct gcagcaggtg cgcttcgaag
     8641 gtttgacagg aaatgtgcag ttcaacgaga aagggcgccg gaccaattac accctccacg
     8701 tgatcgaaat gaaacatgat ggaatccgaa agattggtta ctggaatgaa gacgataaat
     8761 ttgtccccgc agccaccgac gctcaggctg gaggggacaa ctcaagcgtc cagaatagga
     8821 cctacatcgt cactactatc ctcgaagatc cttacgtgat gcttaaaaag aatgccaacc
     8881 agtttgaggg caatgaccgc tatgagggct actgtgtgga gctggctgca gagatcgcca
     8941 agcacgtggg ctactcctac cgacttgaga ttgtcagcga cggcaaatat ggagcccggg
     9001 atcccgacac aaaggcttgg aatggcatgg tgggagaact ggtctatgga agagcagacg
     9061 tggctgtggc tcccttgacc ataaccttgg tccgggagga agtcatcgac ttctccaagc
     9121 cattcatgag tttgggaatc tccattatga ttaagaagcc acagaagtcc aagccaggtg
     9181 tcttctcctt tcttgaccct ttggcctatg agatctggat gtgtatagtg tttgcctaca
     9241 ttggagtgag cgtcgtcctc ttcctggtca gccgtttcag cccctacgaa tggcacagcg
     9301 aagagtttga agagggacga gaccagacaa ccagtgacca gtcaaatgag tttggcatat
     9361 tcaacagcct gtggttctcc ctgggggcct tcatgcagca aggatgtgac atttccccca
     9421 ggtccctgtc cggacgcatc gtcggcggcg tctggtggtt cttcactttg atcatcatct
     9481 cctcgtacac agccaacctg gctgccttcc tgactgtgga gaggatggtg tctcccattg
     9541 agagtgcaga ggacctggca aagcagacgg aaattgctta tgggacattg gaagcaggct
     9601 ccactaagga gttcttcagg agatctaaaa tcgctgtgtt tgagaagatg tggacataca
     9661 tgaagtctgc agaaccatcc gtgtttgttc ggaccacaga ggaaggcatg atcagagtga
     9721 gaaaatctaa aggcaaatac gcctacctcc tggagtccac catgaatgag tatattgagc
     9781 aacgaaagcc ctgtgacacc atgaaagtgg gaggtaactt ggattccaaa ggctatggca
     9841 ttgcgacacc caaggggtcc gccctgagaa atccagtaaa cctggcagtg ttaaaactga
     9901 acgagcaggg gcttttggac aaattgaaaa acaaatggtg gtacgacaag ggcgagtgcg
     9961 gcagcggggg aggtgactcc aaggacaaga ccagcgcttt gagcctcagc aatgtggcag
    10021 gcgtgttcta catcctgatt ggagggctgg gactggccat gctggttgcc ttaatcgagt
    10081 tctgctacaa atcccgtagc gagtcgaagc ggatgaaggg tttctgtttg atcccacagc
    10141 aatccatcaa tgaagccata cggacatcga ccctcccccg gaacagtggg gcaggagcca
    10201 gcggaggagg cggcagtgga gagaatggcc gggtggtcag ccaggacttc cccaagtcca
    10261 tgcaatccat tccctgcatg agtcacagtt cagggatgcc cttgggagcc acaggattgt
    10321 aagctagcta aacaacttcg tatatacctt tctatacgaa gttgtcgaat tcgatatcaa
    10381 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
    10441 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
    10501 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
    10561 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
    10621 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
    10681 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
    10741 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
    10801 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
    10861 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
    10921 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
    10981 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
    11041 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
    11101 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
    11161 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
    11221 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
    11281 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
    11341 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
    11401 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
    11461 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
    11521 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    11581 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    11641 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
    11701 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
    11761 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
    11821 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
    11881 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
    11941 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
    12001 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
    12061 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
    12121 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
    12181 tcgaccccaa aaaacttgat tagggtgatg gttcac
//
