LOCUS       sequence-378604-       12240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of AMPA GluA1 with N terminal HaloTag fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Kim D, Park P, Li X, Wong Campos JD, Tian H, Moult EM, Grimm JB, 
            Lavis L, Cohen AE
  TITLE     Mapping memories: pulse-chase labeling reveals AMPA receptor 
            dynamics during memory formation.
  JOURNAL   bioRxiv. 2023 May 29:2023.05.26.541296. doi: 
            10.1101/2023.05.26.541296. Preprint.
  PUBMED    37292614
REFERENCE   2  (bases 1 to 12240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 May 29:2023.05.26.541296. doi: 10.1101/2023.05.26.541296. 
            Preprint."
COMMENT     Sequence Label: TL023_lenti_CMV:HaloTag-GluA1
FEATURES             Location/Qualifiers
     source          1..12240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    220..337
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        396..699
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        700..903
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     900..924
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4712..5300
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4765..4785)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5183..5194)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5303..5319)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5304..5320)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5825..6005
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6031..6048)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6037..6261
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6307..6735
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6394..6413)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6604..6625
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6744..6764)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6749..7078
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6929..7064
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6991..7010
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7126..7173
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7192..7566
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7696..7817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7733..7752)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7787..7806
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7866..7882)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7866..7882)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7879..7901)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7890..7906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7914..7920,7921..7938,7939..7944))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7959..7980
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8097..8114)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8268..8856)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8348..8367)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9027..9818,9819..9887))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9650..9669
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9888..9992)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10067..10086)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10258..10637
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10639..10837
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             10855..11035
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11082..11207
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11700..11933
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12118..12162
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
       61 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
      121 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
      181 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
      241 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
      301 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
      361 agatccagtt tggttaatta agcttgggag ttccgcgtta cataacttac ggtaaatggc
      421 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      481 atagtaacgc caatagggac tttccattga cgtcaatggg tggagcattt acggtaaact
      541 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      601 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      661 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      721 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      781 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      841 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      901 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      961 agaagacacc gactctagag gatccatgcc gtacatcttt gcctttttct gcaccggttt
     1021 tctaggtgcg gttgtgggtg ccaatgcgcg aggtggaggc ggtgggggag ggggtggagg
     1081 aacgcgtatc ggtactggct ttccattcga cccccattat gtggaagtcc tgggcgagcg
     1141 catgcactac gtcgatgttg gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa
     1201 cccgacctcc tcctacgtgt ggcgcaacat catcccgcat gttgcaccga cccatcgctg
     1261 cattgctcca gacctgatcg gtatgggcaa atccgacaaa ccagacctgg gttatttctt
     1321 cgacgaccac gtccgcttca tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt
     1381 cctggtcatt cacgactggg gctccgctct gggtttccac tgggccaagc gcaatccaga
     1441 gcgcgtcaaa ggtattgcat ttatggagtt catccgccct atcccgacct gggacgaatg
     1501 gccagaattt gcccgcgaga ccttccaggc cttccgcacc accgacgtcg gccgcaagct
     1561 gatcatcgat cagaacgttt ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct
     1621 gactgaagtc gagatggacc attaccgcga gccgttcctg aatcctgttg accgcgagcc
     1681 actgtggcgc ttcccaaacg agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct
     1741 ggtcgaagaa tacatggact ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg
     1801 caccccaggc gttctgatcc caccggccga agccgctcgc ctggccaaaa gcctgcctaa
     1861 ctgcaaggct gtggacatcg gcccgggtct gaatctgctg caagaagaca acccggacct
     1921 gatcggcagc gagatcgcgc gctggctgtc gacgctcgag atttccggcg agccaaccac
     1981 tacgcgtgga ggtggagggg gcggaggtgg cgggggttcg cgcttcccca acaatatcca
     2041 gatagggggg ttatttccaa accaacaatc acaggaacat gcggctttta ggtttgcttt
     2101 gtcacaactc acggagcccc ccaagctgct tccccagatc gatattgtga acatcagcga
     2161 cagctttgag atgacttacc gtttctgttc ccagttctcc aaaggagtct atgccatctt
     2221 tggattttat gaacgaagga ctgtcaacat gctgacctcc ttctgtgggg ccctccatgt
     2281 gtgcttcatt actccaagtt ttcctgttga cacatccaat caatttgtcc ttcagctacg
     2341 cccggaacta caggaagctc tcattagcat tatcgaccat tacaagtggc aaacctttgt
     2401 ctacatttat gatgctgacc ggggcctgtc agtcctgcag agagtcttgg atacagccgc
     2461 agagaagaac tggcaggtaa cggctgtcaa cattctgaca accaccgagg aaggataccg
     2521 gatgctcttt caggacctgg agaagaaaaa ggagaggctg gtggtggttg actgtgaatc
     2581 agaacgcctc aacgccatcc tgggccagat cgtgaagcta gaaaagaatg gcatcgggta
     2641 ccactacatc ctcgccaatc tgggcttcat ggacattgac ttaaataagt tcaaggagag
     2701 cggagccaat gtgacaggtt tccagctggt gaactacaca gacacgatcc cagccagaat
     2761 catgcagcaa tggaggacaa gtgactcccg agaccatacc agggtggact ggaagaggcc
     2821 aaagtacact tctgctctca cctatgatgg tgtcaaggtg atggctgagg ccttccaaag
     2881 cctgcggagg cagaggattg acatatcccg ccgggggaat gctggggact gtctggctaa
     2941 cccagctgtg ccctggggtc aagggatcga catccagaga gccctgcagc aggtgcgctt
     3001 cgaaggtttg acaggaaatg tgcagttcaa cgagaaaggg cgccggacca attacaccct
     3061 ccacgtgatc gaaatgaaac atgatggaat ccgaaagatt ggttactgga atgaagacga
     3121 taaatttgtc cccgcagcca ccgacgctca ggctggaggg gacaactcaa gcgtccagaa
     3181 taggacctac atcgtcacta ctatcctcga agatccttac gtgatgctta aaaagaatgc
     3241 caaccagttt gagggcaatg accgctatga gggctactgt gtggagctgg ctgcagagat
     3301 cgccaagcac gtgggctact cctaccgact tgagattgtc agcgacggca aatatggagc
     3361 ccgggatccc gacacaaagg cttggaatgg catggtggga gaactggtct atggaagagc
     3421 agacgtggct gtggctccct tgaccataac cttggtccgg gaggaagtca tcgacttctc
     3481 caagccattc atgagtttgg gaatctccat tatgattaag aagccacaga agtccaagcc
     3541 aggtgtcttc tcctttcttg accctttggc ctatgagatc tggatgtgta tagtgtttgc
     3601 ctacattgga gtgagcgtcg tcctcttcct ggtcagccgt ttcagcccct acgaatggca
     3661 cagcgaagag tttgaagagg gacgagacca gacaaccagt gaccagtcaa atgagtttgg
     3721 catattcaac agcctgtggt tctccctggg ggccttcatg cagcaaggat gtgacatttc
     3781 ccccaggtcc ctgtccggac gcatcgtcgg cggcgtctgg tggttcttca ctttgatcat
     3841 catctcctcg tacacagcca acctggctgc cttcctgact gtggagagga tggtgtctcc
     3901 cattgagagt gcagaggacc tggcaaagca gacggaaatt gcttatggga cattggaagc
     3961 aggctccact aaggagttct tcaggagatc taaaatcgct gtgtttgaga agatgtggac
     4021 atacatgaag tctgcagaac catccgtgtt tgttcggacc acagaggaag gcatgatcag
     4081 agtgagaaaa tctaaaggca aatacgccta cctcctggag tccaccatga atgagtatat
     4141 tgagcaacga aagccctgtg acaccatgaa agtgggaggt aacttggatt ccaaaggcta
     4201 tggcattgcg acacccaagg ggtccgccct gagaaatcca gtaaacctgg cagtgttaaa
     4261 actgaacgag caggggcttt tggacaaatt gaaaaacaaa tggtggtacg acaagggcga
     4321 gtgcggcagc gggggaggtg actccaagga caagaccagc gctttgagcc tcagcaatgt
     4381 ggcaggcgtg ttctacatcc tgattggagg gctgggactg gccatgctgg ttgccttaat
     4441 cgagttctgc tacaaatccc gtagcgagtc gaagcggatg aagggtttct gtttgatccc
     4501 acagcaatcc atcaatgaag ccatacggac atcgaccctc ccccggaaca gtggggcagg
     4561 agccagcgga ggaggcggca gtggagagaa tggccgggtg gtcagccagg acttccccaa
     4621 gtccatgcaa tccattccct gcatgagtca cagttcaggg atgcccttgg gagccacagg
     4681 attgtaagaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     4741 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4801 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4861 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4921 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4981 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5041 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5101 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5161 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5221 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5281 cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc
     5341 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     5401 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     5461 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5521 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     5581 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     5641 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     5701 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     5761 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     5821 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     5881 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     5941 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6001 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     6061 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     6121 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     6181 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     6241 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     6301 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6361 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6421 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6481 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6541 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6601 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6661 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6721 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     6781 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     6841 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6901 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     6961 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     7021 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     7081 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     7141 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     7201 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     7261 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     7321 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     7381 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     7441 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     7501 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     7561 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     7621 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     7681 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7741 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7801 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     7861 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7921 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7981 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     8041 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8101 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8161 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8221 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8281 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8341 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8401 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8461 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8521 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8581 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8641 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8701 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8761 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8821 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8881 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8941 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9001 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9061 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9121 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9181 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9241 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9301 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9361 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9421 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9481 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9541 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9601 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9661 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9721 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9781 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9841 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9901 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9961 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    10021 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
    10081 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
    10141 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
    10201 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
    10261 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
    10321 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
    10381 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
    10441 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
    10501 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
    10561 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
    10621 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
    10681 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
    10741 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
    10801 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
    10861 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    10921 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    10981 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
    11041 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
    11101 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
    11161 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
    11221 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
    11281 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
    11341 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
    11401 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
    11461 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
    11521 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
    11581 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
    11641 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
    11701 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
    11761 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
    11821 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
    11881 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
    11941 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
    12001 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
    12061 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
    12121 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
    12181 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
//
