LOCUS       sequence-378619-        8699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  broad host plasmid that can be used to construct reproductive 
            success bioreporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Schlechter RO, Kear EJ, Bernach M, Remus DM, Remus-Emsermann MNP
  TITLE     Metabolic resource overlap impacts competition among phyllosphere 
            bacteria.
  JOURNAL   ISME J. 2023 Sep;17(9):1445-1454. doi: 10.1038/s41396-023-01459-0. 
            Epub 2023 Jun 24.
  PUBMED    37355740
REFERENCE   2  (bases 1 to 8699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-023-01459-0"; journalName: "ISME J"; date: 
            "2023-09"; volume: "17"; issue: "9"; pages: "1445-1454"
COMMENT     Sequence Label: pProbe_CUSPER
FEATURES             Location/Qualifiers
     source          1..8699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      97..183
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(522..1316)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(633..652)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1243..1262
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      2796..3567
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             3568..4230
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     4924..4946
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4938..4955
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4939..4955
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4965..4984
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4965..4983
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      5026..5112
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5209..5295
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5392..5478
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5575..5661
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        join(5759..5764,5765..5781,5782..5787)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5976..6509
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        6510..6587
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6588..7670
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6607..6626)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     terminator      complement(7671..7765)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(7782..8495,8496..8498))
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(7827..7848)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8435..8456
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     RBS             8505..8516
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    8535..8551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    8568..8584
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(8630..8648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt
       61 aaacgcctgg ggtaatgact ctctagcttg aggcatcaaa taaaacgaaa ggctcagtcg
      121 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
      181 aatccgccgc taggagcttg cggccgccag tgtgatggat atctgcataa aaactgttgt
      241 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
      301 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggg ggtgggcgaa
      361 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat
      421 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt
      481 gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga
      541 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
      601 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
      661 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
      721 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
      781 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
      841 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
      901 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
      961 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     1021 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     1081 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt
     1141 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     1201 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     1261 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga
     1321 aacgatcctc atcctgtctc ttgatcagat cttgatcccc tgcgccatca gatccttggc
     1381 ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca
     1441 gctggcaatt ccggttcgct tgctgtccat aaaaccgccc agtctagcta tcgccatgta
     1501 agcccactgc aagctacctg ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc
     1561 cagtagctga cattcatccc aggtggcact tttcggggaa atgtgcgcgc ccgcgttcct
     1621 gctggcgctg ggcctgtttc tggcgctgga cttcccgctg ttccgtcagc agcttttcgc
     1681 ccacggcctt gatgatcgcg gcggccttgg cctgcatatc ccgattcaac ggccccaggg
     1741 cgtccagaac gggcttcagg cgctcccgaa ggtctcgggc cgtctcttgg gcttgatcgg
     1801 ccttcttgcg catctcacgc gctcctgcgg cggcctgtag ggcaggctca tacccctgcc
     1861 gaaccgcttt tgtcagccgg tcggccacgg cttccggcgt ctcaacgcgc tttgagattc
     1921 ccagcttttc ggccaatccc tgcggtgcat aggcgcgtgg ctcgaccgct tgcgggctga
     1981 tggtgacgtg gcccactggt ggccgctcca gggcctcgta gaacgcctga atgcgcgtgt
     2041 gacgtgcctt gctgccctcg atgccccgtt gcagccctag atcggccaca gcggccgcaa
     2101 acgtggtctg gtcgcgggtc atctgcgctt tgttgccgat gaactccttg gccgacagcc
     2161 tgccgtcctg cgtcagcggc accacgaacg cggtcatgtg cgggctggtt tcgtcacggt
     2221 ggatgctggc cgtcacgatg cgatccgccc cgtacttgtc cgccagccac ttgtgcgcct
     2281 tctcgaagaa cgccgcctgc tgttcttggc tggccgactt ccaccattcc gggctggccg
     2341 tcatgacgta ctcgaccgcc aacacagcgt ccttgcgccg cttctctggc agcaactcgc
     2401 gcagtcggcc catcgcttca tcggtgctgc tggccgccca gtgctcgttc tctggcgtcc
     2461 tgctggcgtc agcgttgggc gtctcgcgct cgcggtaggc gtgcttgaga ctggccgcca
     2521 cgttgcccat tttcgccagc ttcttgcatc gcatgatcgc gtatgccgcc atgcctgccc
     2581 ctcccttttg gtgtccaacc ggctcgacgg gggcagcgca aggcggtgcc tccggcgggc
     2641 cactcaatgc ttgagtatac tcactagact ttgcttcgca aagtcgtgac cgcctacggc
     2701 ggctgcggcg ccctacgggc ttgctctccg ggcttcgccc tgcgcggtcg ctgcgctccc
     2761 ttgccagccc gtggatatgt ggacgatggc cgcgagcggc caccggctgg ctcgcttcgc
     2821 tcggcccgtg gacaaccctg ctggacaagc tgatggacag gctgcgcctg cccacgagct
     2881 tgaccacagg gattgcccac cggctaccca gccttcgacc acatacccac cggctccaac
     2941 tgcgcggcct gcggccttgc cccatcaatt tttttaattt tctctgggga aaagcctccg
     3001 gcctgcggcc tgcgcgcttc gcttgccggt tggacaccaa gtggaaggcg ggtcaaggct
     3061 cgcgcagcga ccgcgcagcg gcttggcctt gacgcgcctg gaacgaccca agcctatgcg
     3121 agtgggggca gtcgaagggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag
     3181 tgcgggggtt ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc
     3241 aacaagcccc ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga
     3301 accccgcagg ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa
     3361 gacttaaaaa tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc
     3421 gcaatagctc attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg
     3481 cataattgtt gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg
     3541 cggcacccct accgcatgga gataagcatg gccacgcagt ccagagaaat cggcattcaa
     3601 gccaagaaca agcccggtca ctgggtgcaa acggaacgca aagcgcatga ggcgtgggcc
     3661 gggcttattg cgaggaaacc cacggcggca atgctgctgc atcacctcgt ggcgcagatg
     3721 ggccaccaga acgccgtggt ggtcagccag aagacacttt ccaagctcat cggacgttct
     3781 ttgcggacgg tccaatacgc agtcaaggac ttggtggccg agcgctggat ctccgtcgtg
     3841 aagctcaacg gccccggcac cgtgtcggcc tacgtggtca atgaccgcgt ggcgtggggc
     3901 cagccccgcg accagttgcg cctgtcggtg ttcagtgccg ccgtggtggt tgatcacgac
     3961 gaccaggacg aatcgctgtt ggggcatggc gacctgcgcc gcatcccgac cctgtatccg
     4021 ggcgagcagc aactaccgac cggccccggc gaggagccgc ccagccagcc cggcattccg
     4081 ggcatggaac cagacctgcc agccttgacc gaaacggagg aatgggaacg gcgcgggcag
     4141 cagcgcctgc cgatgcccga tgagccgtgt tttctggacg atggcgagcc gttggagccg
     4201 ccgacacggg tcacgctgcc gcgccggtag cacttgggtt gcgcagcaac ccgtaagtgc
     4261 gctgttccag actatcggct gtagccgcct cgccgcccta taccttgtct gcctccccgc
     4321 gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg gcggcacctc
     4381 gctaacggat tcaccgtttt tatcaggctc tgggaggcag aataaatgat catatcgtca
     4441 attattacct ccacggggag agcctgagca aactggcctc aggcatttga gaagcacacg
     4501 gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt
     4561 taacgaccct gccctgaacc gacgaccggg tcgaatttgc tttcgaattt ctgccattca
     4621 tccgcttatt atcacttatt caggcgtagc aaccaggcgt ttaagggcac caataactgc
     4681 cttaaaaaaa ttacgccccg ccctgccact catcgcagta cggcctattg gttaaaaaat
     4741 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
     4801 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     4861 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     4921 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     4981 agggcgaatt ggagctgggg ccgcaattcc caattcccca ggcatcaaat aaaacgaaag
     5041 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     5101 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     5161 cgggcaggac gcccgccata aactgccagg aattaattcc ccaggcatca aataaaacga
     5221 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     5281 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     5341 tggcgggcag gacgcccgcc ataaactgcc aggaattaat tccccaggca tcaaataaaa
     5401 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     5461 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga
     5521 gggtggcggg caggacgccc gccataaact gccaggaatt aattccccag gcatcaaata
     5581 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     5641 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc
     5701 ggagggtggc gggcaggacg cccgccataa actgccagga attggggatc ggaagctttt
     5761 gacataagcc tgttcggttc gtaaactgta atgcaagtag cgtatgcgct cacgcaactg
     5821 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     5881 ttatgactgt ttttttgtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     5941 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagc aacgatgtta
     6001 cgcagcaggg cagtcgccct aaaacaaagt taggtggctc aagtatgggc atcattcgca
     6061 catgtaggct cggccctgac caagtcaaat ccatgcgggc tgctcttgat cttttcggtc
     6121 gtgagttcgg agacgtagcc acctactccc aacatcagcc ggactccgat tacctcggga
     6181 acttgctccg tagtaagaca ttcatcgcgc ttgctgcctt cgaccaagaa gcggttgttg
     6241 gcgctctcgc ggcttacgtt ctgcccaagt ttgagcagcc gcgtagtgag atctatatct
     6301 atgatctcgc agtctccggc gagcaccgga ggcagggcat tgccaccgcg ctcatcaatc
     6361 tcctcaagca tgaggccaac gcgcttggtg cttatgtgat ctacgtgcaa gcagattacg
     6421 gtgacgatcc cgcagtggct ctctatacaa agttgggcat acgggaagaa gtgatgcact
     6481 ttgatatcga cccaagtacc gccacctaag acaccatcga atggtgcaaa acctttcgcg
     6541 gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa
     6601 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga
     6661 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc
     6721 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg
     6781 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat
     6841 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg
     6901 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta
     6961 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg
     7021 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag
     7081 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt
     7141 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc
     7201 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg
     7261 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg
     7321 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg
     7381 gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc
     7441 cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc
     7501 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga
     7561 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7621 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga attctcacca
     7681 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     7741 attactggat ctatcaacag gagtccaagc tcagctaatt attacttgta cagctcgtcc
     7801 atgccatgtg taatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
     7861 tcgttggggt ctttgctcag cttggactga tggctcaggt agtggttgtc gggcagcagc
     7921 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
     7981 tcctcaacgt tgtggcggat cttgaagtta gccttgatgc agttcttctg cttgtcggcc
     8041 gtgatataga cgtagtggct gttgaagttg tactccagct tgtgccccag gatgttgccg
     8101 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
     8161 ttcacctcgg cgcgggtctt gtaggtaccg tcgtccttga aagagatggt gcgctcctgg
     8221 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
     8281 cggctgaagc aggccacgcc gtagccgaag gtggtcacga gggtgggcca gggcacgggc
     8341 agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgttggtggc atcgccctcg
     8401 ccctcgccgc ggacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
     8461 ggcaccaccc cggtgaacag ctcctcgccc ttgctcatgc ttaatttctc ctctttaatt
     8521 ctagatgtgt gaaattgtta tccgctcaca attgaatcta agtatcattg ttatccgctc
     8581 acaagtcaac actctttttg ataaattttc tcgaggtgaa gacgaaaggg cctcgtgata
     8641 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcac
//
