LOCUS       sequence-378699-        2740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#91.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM31
FEATURES             Location/Qualifiers
     source          1..2740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(627..726)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(783..799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(783..799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(796..818)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(971..1559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1051..1070)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1695..2510)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1695..2510)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2418..2437
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2502..2521
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2631..2647
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2631..2647
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2648..2694
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gccgtccact cggttcgacc tgaggccagg
      301 ggagtctatg gcaaccatcc agcgacccct catcctgcca cactcggaga ggcggcggac
      361 ggatgatggc tacaactggc gcaagtatgg gcagacacag gtgaagggga gtgagaaccc
      421 gagaagctac tacaaatgtg ccttccccag ctgtcccgcc aagaagaagg tggagaggtc
      481 gctcgacaat cagataattg agatcctcta caagggtaga cacaatcacc ccaagcccca
      541 caacacggac agtgaaaatt ttggggacgc caacagtggt atctcaggca caacctttga
      601 ggaacctggg agtttggacg atcacgaccc agctttcttg tacaaagttg gcattataag
      661 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
      721 tatttgaggc taggtggagg ctcagtgatg ataagtctgc gatggtggat gcatgtgtca
      781 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca gagggcacaa tcctattccg
      841 cgctatccga caatctccaa gacattaggt ggagttcagt tcggcgtatg gcatatgtcg
      901 ctggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      961 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1021 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1081 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1141 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1201 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1261 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1321 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1381 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1441 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1501 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1561 aagatccttt gatcttttct acggggtctg acgctctatt caacaaagcc gccgtcccgt
     1621 caagtcagcg taaatgggta gggggcttca aatcgtccgc tctgccagtg ttacaaccaa
     1681 ttaacaaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     1741 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     1801 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     1861 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     1921 ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact
     1981 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     2041 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta
     2101 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     2161 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg
     2221 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     2281 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
     2341 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca
     2401 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
     2461 gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa tatggctcat aacacccctt
     2521 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
     2581 gcaatgtaac atcagagatt ttgagacaca acgtggcttt cccccgccgc tctagaacta
     2641 gtggatccaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2701 tttgtcgcat tatacgagac gtccaggttg ggatacctga
//
