LOCUS       sequence-378700-        2845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#60.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM34
FEATURES             Location/Qualifiers
     source          1..2845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             338..349
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(732..831)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(888..904)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(888..904)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(901..923)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1076..1664)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1156..1175)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1800..2615)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1800..2615)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2523..2542
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2607..2626
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2736..2752
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2736..2752
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2753..2799
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gacaccgaat agcagtgttg gcaaattacg
      301 gtccgcggta tggcaacact ttataatcac agaaaaaatt gaaggaaggc cggtcagagc
      361 aaaatgcagt ctctgttcag aagagtttgc gtgcgattca gtggcgaacg ggacatcatc
      421 tatgagaaag catttaaaga acgcgcattc cgtaatttgc gaaaatggaa agcgttcacg
      481 aaaccattca agcacgggtg atgatgctac tgaaaatgcg acgcctattg tcattggcag
      541 ctcatccagg ggaaaaagaa agagaacaaa tgaggactcg gcacaaatca ccgcagctaa
      601 caaacgcacc cattgggaca aggcggaaat atccaatagg ataaaggaaa taactagtca
      661 gttaaaagac atccgagagg aagtgagtga ggttctcaag ctgcatggat cagaccttgc
      721 ttcaagttca gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
      781 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gaggctaggt
      841 ggaggctcag tgatgataag tctgcgatgg tggatgcatg tgtcatggtc atagctgttt
      901 cctgtgtgaa attgttatcc gctcagaggg cacaatccta ttccgcgcta tccgacaatc
      961 tccaagacat taggtggagt tcagttcggc gtatggcata tgtcgctgga aagaacatgt
     1021 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1081 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1141 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1201 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1261 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1321 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1381 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1441 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1501 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1561 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1621 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1681 tttctacggg gtctgacgct ctattcaaca aagccgccgt cccgtcaagt cagcgtaaat
     1741 gggtaggggg cttcaaatcg tccgctctgc cagtgttaca accaattaac aaattctgat
     1801 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     1861 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     1921 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     1981 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     2041 gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag
     2101 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     2161 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     2221 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     2281 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
     2341 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     2401 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     2461 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     2521 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     2581 ctcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     2641 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
     2701 agattttgag acacaacgtg gctttccccc gccgctctag aactagtgga tccaaataaa
     2761 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cgcattatac
     2821 gagacgtcca ggttgggata cctga
//
