LOCUS       sequence-378705-        3256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#82.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM39
FEATURES             Location/Qualifiers
     source          1..3256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1143..1242)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1299..1315)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1299..1315)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1312..1334)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1487..2075)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1567..1586)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2211..3026)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2211..3026)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2934..2953
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3018..3037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3147..3163
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3147..3163
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      3164..3210
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gtggaagtgc aaccattgta aacatgaatt
      301 tgccggaggt gctacaagaa ttgaagagca tatcactggc aaaggaaaca atattacaaa
      361 atgcccaaaa tatggtgccc atgaagaaga tgaaattaac ttgggagacc agatagtgga
      421 gcatgattct gctgcatttg aaaaggcgtt tttttatcca aagggggacc ctgatgctgt
      481 ttgtatcaga aatagcgata ttgagctttt acagcctgag aaatgcctta atgataatat
      541 cattgacttt tatatcaatt atttgaaaaa taaactccca actgataaac aggacaggtt
      601 tcactttttc aattgctttt tctttcctaa gcttgttgat ttaagcacag ataatccatc
      661 aattgcttct gatggtaaag cagcatttca gcgtgtaagc aaattgacaa gaaaagtgaa
      721 cctttttaaa acggattata tcttcattcc cataaactat agtcttcact ggagtttgat
      781 tgtcatttgt caccctgctg aagtcatgac atgctacaga gatgaagaaa ctaagggatc
      841 tcccaaagaa gcttgcatct tgcacatgga ttcccgaaaa ggaattcatc aagatctcca
      901 caatgttttc caaagttatc tatgtgaaga atggaaagag aggcacaaca atgtgaggga
      961 tgatgatgtt tcttctatat ttttacatct tccattcgtg ccacttgagc tgcctcagca
     1021 acaaaatgca tacgattgtg gcatcttttt gctccactat gtggaacgtt ttctggaaca
     1081 agctccaatc aacttcaacc gttccatgat aagcaagttc agttattggt tccctccacc
     1141 agacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     1201 caacgaacag gtcactatca gtcaaaataa aatcattatt tgaggctagg tggaggctca
     1261 gtgatgataa gtctgcgatg gtggatgcat gtgtcatggt catagctgtt tcctgtgtga
     1321 aattgttatc cgctcagagg gcacaatcct attccgcgct atccgacaat ctccaagaca
     1381 ttaggtggag ttcagttcgg cgtatggcat atgtcgctgg aaagaacatg tgagcaaaag
     1441 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1501 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1561 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1621 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1681 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1741 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1801 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1861 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1921 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1981 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2041 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2101 ggtctgacgc tctattcaac aaagccgccg tcccgtcaag tcagcgtaaa tgggtagggg
     2161 gcttcaaatc gtccgctctg ccagtgttac aaccaattaa caaattctga ttagaaaaac
     2221 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
     2281 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
     2341 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
     2401 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
     2461 gagaatggca aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc
     2521 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
     2581 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
     2641 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
     2701 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta
     2761 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     2821 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     2881 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
     2941 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa
     3001 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     3061 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
     3121 gacacaacgt ggctttcccc cgccgctcta gaactagtgg atccaaataa aacgaaaggc
     3181 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcgcattata cgagacgtcc
     3241 aggttgggat acctga
//
