LOCUS       sequence-378707-        3346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#84.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM41
FEATURES             Location/Qualifiers
     source          1..3346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1233..1332)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1389..1405)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1389..1405)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1402..1424)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1577..2165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1657..1676)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2301..3116)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2301..3116)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3024..3043
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3108..3127
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3237..3253
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3237..3253
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      3254..3300
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gagaaaaaga aaaaggggag gtcggcccaa
      301 aagtaattac tggaatgaag ttgaaaaaat aggtgacatg tggaagtgca accattgtga
      361 aggggagttc catggaggtg ctacaagaat taaagagcat atcattggtg gaggaaaaaa
      421 tattagaaaa tgcccccact atcctgcttc cggttcaatt acacggaatc aattacaaga
      481 agatgtggag catgattctg ctgcatttaa aagcaatgac atggaaaagg caatttgtta
      541 tccaaagggg gaccctgatg ctatttacat caggaagagc gatattgagc ttttacagcc
      601 tgagaaatgc cttaatgata atatcattga cttttatata aagtatttga taaataataa
      661 actcccaagt gataaacaga agaggtttca ctttttcaat tgctttttct ttcctaagct
      721 tgttgattta agcacagata atcgttcaat tgcttgtgat ggtaaagcag catttcagcg
      781 tgtaagcaat tggacaagaa aagtgaacct ttttgaaaag gattatatct tcattcccat
      841 aaactatagt cttcattgga gtttgattgt catttgtcac cctgctgaag tcatgacatg
      901 ctacagagat gaagaaacta agggatctcc caaagaagct tgcatcttgc acatggattc
      961 ccgaaaagga attcatcaag atctccacaa tgttttccaa agttatctat gtgaagaatg
     1021 gaaagagagg cacaacaatg tgagggatga tgcttcttct aaatttttag atcttccatt
     1081 cgtgccactt gagctgcctc agcaacaaaa tgcatacgat tgtggcatct ttttgctcca
     1141 ctatgtggaa cgttttctgg aaaaagctcc aatcaacttt aaccgttccc tgataaccaa
     1201 cttcagttct tggttccctc cactagatgc tgacccagct ttcttgtaca aagttggcat
     1261 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     1321 aatcattatt tgaggctagg tggaggctca gtgatgataa gtctgcgatg gtggatgcat
     1381 gtgtcatggt catagctgtt tcctgtgtga aattgttatc cgctcagagg gcacaatcct
     1441 attccgcgct atccgacaat ctccaagaca ttaggtggag ttcagttcgg cgtatggcat
     1501 atgtcgctgg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1561 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1621 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1681 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1741 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1801 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1861 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1921 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1981 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     2041 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2101 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2161 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tctattcaac aaagccgccg
     2221 tcccgtcaag tcagcgtaaa tgggtagggg gcttcaaatc gtccgctctg ccagtgttac
     2281 aaccaattaa caaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     2341 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     2401 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     2461 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     2521 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc
     2581 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     2641 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     2701 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     2761 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca
     2821 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     2881 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     2941 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
     3001 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     3061 atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg gctcataaca
     3121 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
     3181 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttcccc cgccgctcta
     3241 gaactagtgg atccaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     3301 ctgttgtttg tcgcattata cgagacgtcc aggttgggat acctga
//
