LOCUS       sequence-378708-        2743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#54.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM44
FEATURES             Location/Qualifiers
     source          1..2743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(630..729)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(786..802)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(786..802)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(799..821)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(974..1562)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1054..1073)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1698..2513)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1698..2513)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2421..2440
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2505..2524
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2634..2650
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2634..2650
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2651..2697
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat ggctccacgt tacgacggcg attccttcaa
      301 gaaggggcca tggacgccgg aggaggacaa gcttctcatc gactacatcg gggctcatgg
      361 ctcttgcagg tggaacagca tcgcgaggct cgccggtctt aaccgcaccg ggaagagctg
      421 ccggctccgt tggctcaact gcctacagcc cgacatcaag cgcgtccact tcaccgacga
      481 tgaggagaaa ctcctcatca acctccactc ctccctcggg aacaagtggt cggagatcgg
      541 gacgcatatg cctggaagga cggacaacga gatcaagaac tactggaaca cgcacctaag
      601 caagaagctc ccggccggca tcgtcaagga cccagctttc ttgtacaaag ttggcattat
      661 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
      721 cattatttga ggctaggtgg aggctcagtg atgataagtc tgcgatggtg gatgcatgtg
      781 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcagagggca caatcctatt
      841 ccgcgctatc cgacaatctc caagacatta ggtggagttc agttcggcgt atggcatatg
      901 tcgctggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      961 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1021 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1081 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1141 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1201 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1261 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1321 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1381 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1441 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1501 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1561 aagaagatcc tttgatcttt tctacggggt ctgacgctct attcaacaaa gccgccgtcc
     1621 cgtcaagtca gcgtaaatgg gtagggggct tcaaatcgtc cgctctgcca gtgttacaac
     1681 caattaacaa attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc
     1741 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     1801 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     1861 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     1921 tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag
     1981 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     2041 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     2101 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     2161 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
     2221 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     2281 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     2341 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     2401 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     2461 ttggaattta atcgcggcct cgagcaagac gtttcccgtt gaatatggct cataacaccc
     2521 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     2581 tgtgcaatgt aacatcagag attttgagac acaacgtggc tttcccccgc cgctctagaa
     2641 ctagtggatc caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     2701 ttgtttgtcg cattatacga gacgtccagg ttgggatacc tga
//
