LOCUS       sequence-378710-        9899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9899)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 9899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: CASR-Tango
FEATURES             Location/Qualifiers
     source          1..9899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        95..474
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        475..678
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     629..649
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     723..742
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        723..741
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4317..4931,4932..5318)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
                     LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
                     LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(4384..4403)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(5350..5367)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5356..5580
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5626..6054
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5713..5732)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5923..5944
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6063..6083)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6068..6397
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6248..6383
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6310..6329
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6464..7258
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6518..6537)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7128..7147
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7432..7553
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7469..7488)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7523..7542
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7602..7618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7602..7618)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7615..7637)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7626..7642
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7650..7656,7657..7674,7675..7680))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7695..7716
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7833..7850)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8004..8592)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8084..8103)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8763..9554,9555..9623))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9386..9405
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9624..9728)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9803..9822)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
       61 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
      121 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      181 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      241 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      301 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      361 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      421 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      481 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      541 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      601 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      661 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa
      721 attaatacga ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta
      781 ccgagctcgg atccactagt ccagtgtggt ggaattctgc agatatccag cacagtggcg
      841 gccgcgccac catgaagacg atcatcgccc tgagctacat cttctgcctg gtattcgccg
      901 actacaagga cgatgatgac gccagcatcg atatggcctt ctattcttgt tgctgggtac
      961 ttctggcgct tacctggcac acttctgcat atggccctga ccaacgcgcc cagaaaaaag
     1021 gcgatataat tctcgggggc ttgttcccta tccacttcgg cgtagctgcc aaggatcaag
     1081 acttgaaatc acggccagag tcagtggagt gtattaggta caactttagg ggatttcggt
     1141 ggctccaggc tatgattttc gccattgaag aaatcaactc atctcccgcc ttgttgccaa
     1201 acctgacctt gggctaccga attttcgata catgcaacac tgtctccaag gctctcgaag
     1261 caaccctcag ctttgtcgcc cagaataaga ttgattcctt gaatcttgat gagttttgca
     1321 actgctccga acacatacca tcaactattg ccgtggttgg cgccactggc agtggcgtga
     1381 gtactgctgt cgcaaatctc ctcggtttgt tctacatacc tcaggtctcc tacgctagct
     1441 cctcacgatt gctgagcaac aagaaccagt ttaaatcatt cttgcgcact atccccaatg
     1501 acgaacatca ggccacggcc atggcagaca ttattgaata tttccgatgg aactgggtcg
     1561 ggacaatcgc tgccgatgat gattatggcc gccccggtat cgagaagttt cgggaagaag
     1621 ccgaggagcg ggatatctgc attgatttta gcgagctgat cagtcagtac tccgacgaag
     1681 aagaaatcca acacgtagta gaggttatcc agaactctac cgctaaggtg atagtcgtgt
     1741 tttcaagcgg cccagatctg gagcctttga tcaaggagat cgttcgaagg aatatcacag
     1801 gaaagatttg gctcgctagc gaggcttggg caagcagtag ccttatcgca atgcctcaat
     1861 atttccatgt ggtggggggc acaattggtt tcgctctgaa ggccgggcag atcccagggt
     1921 tcagggaatt cctgaagaag gtgcatcctc ggaagtctgt gcacaacgga ttcgccaagg
     1981 agttctggga ggaaactttt aattgtcacc tccaggaggg agccaaggga cctctccctg
     2041 ttgatacttt cctgaggggg catgaagaat ccggtgatcg cttctctaac tcttccacag
     2101 cctttagacc tctgtgcaca ggcgatgaga atatatcctc cgtggaaacc ccttatatcg
     2161 actataccca tcttcggatc agctataacg tttatctcgc cgtgtactca atcgcccacg
     2221 ccctgcagga catttatacg tgtctccctg ggagaggctt gtttactaac gggtcatgcg
     2281 ctgatatcaa gaaggttgaa gcctggcagg tgctcaagca tctgaggcat ctgaacttca
     2341 caaataatat gggagagcag gtcactttcg atgaatgtgg tgatttggtc ggaaattaca
     2401 gcattatcaa ttggcatctg tcccctgaag atggctccat tgtgtttaaa gaagttggct
     2461 actacaatgt ttacgctaaa aaaggtgaga ggctgtttat caacgaggag aaaatactgt
     2521 ggagcggatt ttcacgcgaa cccttgacct ttgtcttgag cgttcttcag gtccccttct
     2581 ccaattgctc ccgcgactgt ctggctggca cccggaaagg gatcattgag ggtgaaccaa
     2641 cctgctgctt cgagtgtgta gaatgcccag acggtgaata cagcgacgaa accgacgcct
     2701 ctgcctgtaa caagtgcccg gatgatttct ggagtaacga gaatcataca tcatgtatcg
     2761 ctaaagaaat tgagtttctg tcttggacgg aacctttcgg catagccctc accctctttg
     2821 ccgttctggg tatctttctt accgcatttg tactgggagt gttcattaag ttcaggaaca
     2881 cccccatcgt caaggcaacc aaccgggaac tctcctatct gctcctgttt agtcttctgt
     2941 gctgtttctc atccagtttg ttcttcatcg gtgagcccca ggattggacc tgcaggctcc
     3001 gccagccagc ttttggcatc agtttcgtcc tgtgcatctc ctgcattctc gtaaagacta
     3061 atagggtctt gttggtgttc gaagccaaaa tacctacaag ttttcatagg aaatggtggg
     3121 gtctgaatct gcagttcttg ttggttttcc tgtgcacatt catgcagatc gtcatctgcg
     3181 tcatttggct gtataccgcc cccccgtcct cctaccgcaa tcaggagctg gaagacgaga
     3241 ttatctttat cacctgtcac gagggtagcc tgatggcgct gggatttctg atcggttaca
     3301 catgtctgct ggctgcaatc tgctttttct tcgccttcaa gagtcggaaa ctgcccgaga
     3361 attttaatga ggccaagttc atcaccttct caatgcttat attcttcata gtttggataa
     3421 gttttattcc cgcctatgcc agtacgtatg gcaaattcgt cagcgcggtc gaggttatcg
     3481 ccatcctggc cgcttctttt ggcctgttgg cctgtatttt ctttaacaag atctacatca
     3541 tcttgttcaa gccctcaaga aataccatcg aagaagtcag gtgtagtact gccgcacacg
     3601 cctttaaagt cgccgcacgc gccacgctca ggagatccaa cgtcagcagg aaaaggagct
     3661 cctctctcgg gggtagtact gggtctaccc ccagctcctc tatcagctca aaatctaact
     3721 ctgaagaccc attcccacag cccgaaagac agaaacagca acagccactt gctctgaccc
     3781 agcaagagca gcagcagcaa cctcttacat tgcctcaaca gcaacggagt cagcagcagc
     3841 ccaggtgtaa gcagaaggtg attttcggct ccggtactgt gacgttctca ctgagttttg
     3901 acgagccaca gaagaatgcc atggcgcaca gaaacagcac acaccagaat agcctcgaag
     3961 cacagaaatc atctgataca ctcacaaggc accaacccct gctgcctctc cagtgtgggg
     4021 aaaccgatct cgatttgacg gtccaggaga ctggactcca gggaccagtg ggcggcgacc
     4081 agcggcctga agttgaggat cccgaggaat tgagccccgc cctggtggtt agttccagtc
     4141 agtcctttgt catctcagga ggaggtagta ccgtgaccga aaacgtagtc aattctatcg
     4201 ataccggtgg acgcacccca cccagcctgg gtccccaaga tgagtcctgc accaccgcca
     4261 gctcctccct ggccaaggac acttcatcga ccggtgagaa cctgtacttc cagctaagat
     4321 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
     4381 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
     4441 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
     4501 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta
     4561 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac
     4621 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag
     4681 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt
     4741 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg
     4801 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc
     4861 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg
     4921 aaagtgggtc cgcgtacagc cgcgcgcgta cgaaaaacaa ttacgggtct accatcgagg
     4981 gcctgctcga tctcccggac gacgacgccc ccgaagaggc ggggctggcg gctccgcgcc
     5041 tgtcctttct ccccgcggga cacacgcgca gactgtcgac ggcccccccg accgatgtca
     5101 gcctggggga cgagctccac ttagacggcg aggacgtggc gatggcgcat gccgacgcgc
     5161 tagacgattt cgatctggac atgttggggg acggggattc cccgggtccg ggatttaccc
     5221 cccacgactc cgccccctac ggcgctctgg atatggccga cttcgagttt gagcagatgt
     5281 ttaccgatgc ccttggaatt gacgagtacg gtgggtagtc tagagggccc gtttaaaccc
     5341 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     5401 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     5461 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     5521 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     5581 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     5641 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     5701 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5761 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5821 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     5881 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5941 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6001 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     6061 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6121 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6181 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6241 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6301 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     6361 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     6421 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     6481 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     6541 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     6601 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     6661 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     6721 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     6781 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     6841 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     6901 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     6961 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     7021 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     7081 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     7141 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     7201 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     7261 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     7321 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     7381 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     7441 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7501 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7561 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     7621 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     7681 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     7741 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7801 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7861 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7921 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7981 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8041 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8101 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8161 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8221 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8281 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8341 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8401 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     8461 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8521 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8581 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8641 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8701 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8761 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8821 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8881 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8941 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9001 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9061 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9121 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9181 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9241 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9301 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9361 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9421 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9481 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9541 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9601 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9661 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9721 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     9781 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     9841 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctaca
//
