LOCUS       sequence-378711-        3244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#59.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM47
FEATURES             Location/Qualifiers
     source          1..3244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1131..1230)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1287..1303)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1287..1303)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1300..1322)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1475..2063)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1555..1574)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2199..3014)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2199..3014)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2922..2941
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3006..3025
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3135..3151
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3135..3151
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      3152..3198
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gccttccaat tgtgtctggg aggattcttc
      301 tgcaatggtt tacaagggga ctcttaatga aaaaatgaag ccatcaagga gcagcagttt
      361 tctggtggtt gctgtcaaga agttcaagcc tgagagcata tgctggcctg aaacttcaaa
      421 gaatgagatg gatatttttg gaagaatttc acacccaaac cttgtcaaac tcttaggcta
      481 ctgcttggag gacaacaaca cagcccttgt atacgagttc atggcaaggg ggagcttgca
      541 gaatcacctc tttcggagag aaacgaagca tctatcttgg agtctcaggg tcaatattct
      601 tattggggca gctcgtggcc ttgccttcct tcactcacta gaaagaccaa tcatccacgg
      661 agactttaag gcctcaaaaa tcctattaga ttcgaactat aatgcaaagc tctcgggtct
      721 tggagccgcg aaggatggcc tgtatggtgg gccatcacat ttaacagcac gagtcgtggg
      781 cacatatgcg tatgcacctc cagaatatgt agcaacaggg cgcctgtatg tgaagagtga
      841 tgtctataac tttggagttg tgctcctgga gatgctctct ggtctgagag cgatcgaccc
      901 aagccgcccc accgggatgc tcagcttggt ggattgggcg aaaccgtacc tgtctgacag
      961 gagaaggcta catagggtaa tagatcctcg tcttgcgggg gagtactcat ccagaggagc
     1021 cctgcaggct tcacagctcg cactcagctg ccttcgtaat gaacatagtt tacgtccatc
     1081 aatgaatgaa gtcgtggatg ttctcgagag tataggcttc atggataccg acccagcttt
     1141 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     1201 cactatcagt caaaataaaa tcattatttg aggctaggtg gaggctcagt gatgataagt
     1261 ctgcgatggt ggatgcatgt gtcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     1321 ctcagagggc acaatcctat tccgcgctat ccgacaatct ccaagacatt aggtggagtt
     1381 cagttcggcg tatggcatat gtcgctggaa agaacatgtg agcaaaaggc cagcaaaagg
     1441 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1501 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1561 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1621 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1681 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1741 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1801 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1861 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1921 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1981 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2041 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2101 tattcaacaa agccgccgtc ccgtcaagtc agcgtaaatg ggtagggggc ttcaaatcgt
     2161 ccgctctgcc agtgttacaa ccaattaaca aattctgatt agaaaaactc atcgagcatc
     2221 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     2281 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     2341 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     2401 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     2461 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     2521 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     2581 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     2641 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     2701 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     2761 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     2821 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     2881 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     2941 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt
     3001 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     3061 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     3121 ctttcccccg ccgctctaga actagtggat ccaaataaaa cgaaaggctc agtcgaaaga
     3181 ctgggccttt cgttttatct gttgtttgtc gcattatacg agacgtccag gttgggatac
     3241 ctga
//
