LOCUS       sequence-378713-        4198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#57.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM61
FEATURES             Location/Qualifiers
     source          1..4198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2085..2184)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2241..2257)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2241..2257)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2254..2276)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2429..3017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2509..2528)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3153..3968)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3153..3968)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3876..3895
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3960..3979
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     4089..4105
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4089..4105
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      4106..4152
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gaacagctac acctttactg atgggagggt
      301 gctgtgttta gaagatcgag tagccattgc gctgataatg ctcaactctg gcgagccact
      361 ggaggccgta gcgttgtctg ttggtgtgaa cgagtcaacc atctcgctgg taacagagag
      421 gtttattgat gctacgtggg agcaagcaga tcaccatttg aactggccag gctccagtga
      481 aatagagaag gtcaagtcca tgtttgacaa gattcacggt ctcccaaact gctgcggtat
      541 tatatgtaca acgcacatca tatttggatc acaaaactgc aataatgaga cgaatgacga
      601 tattatagtg caagtcgttg ttgatccaga tatgaggttc aacaacattt ggttgggagg
      661 gtcagatgcc atgaaccaga tgagcctttt gcacgactct cagttcttca aggagtgtga
      721 cgcgggtgct ttggtaaatg gcagcaagct cgagttatca tccaatgggt cagaagaagt
      781 tgaggaatat gtaattggtg ctgcaggata ccctcttcgt ccttggctcc tcacacctta
      841 caagcagcaa aacatggatg aactcttgga ttctcaagtg gagttcaacc ggagacactc
      901 tgcagctcta acctttgtcc tgaaggtgtt ggcaaggttg aaagacacat ggaagtgcct
      961 gcatggaggg atgtggcacc cggaagatcc aaaggagctg tctcgggtaa tctatgtgtg
     1021 catcacgttg cataacatag tgatagacat ggaggaggcg cccatggtga ggcctcttgt
     1081 ggtgaattac agcaagcgag tgcgcagatt agcagatgag gatgctgtca gtgtaaggga
     1141 tttactgtcg caacatttta tcggaaggtc ctcccaatct caaggaacct tatatctttt
     1201 tgaggactca caaatgtttg agtctgtcct caaaatgaca aggagaacct tcagctacat
     1261 ctgcagcttg gtgaaggtgc cgtctttgaa agatatgaac aactacacct ttattgatgg
     1321 gagggtgctg tgtttagaag atcgagtagc tgtcgctctg ataatgttga acgctggcca
     1381 gacactggat gacgtgggat cctctgttgg tgtgaacaag tcaaccgtct cactggtaac
     1441 tgagaggttt gttgatgcta tgcgggagcg agcaaggcac cacatgaagt ggccaggttc
     1501 tggcgaaatg gagaacgtca agtccaaatt tgacaagatc cttggtctgc caaactgctg
     1561 tggtgttgta catacaagtc acatcccatt tggatcagaa aactgcgacc atgagagaaa
     1621 tttctgtgtg ctaatgcaag ccgtcgtcga tccagatatg aggttcagaa acatttggca
     1681 aggatggtca gacaggacaa accagttggg tcttttgcac aactctgagc tcttcaaaga
     1741 gtgtgaaaac ggtgcttggc ttaacggaag caagctagag gtatcatcag aaggatcaga
     1801 agttggggag tatgtaattg gtgatgcagg ataccctctt ctcccctggc tcctaacacc
     1861 ttaccagcag aaagacacgg atgaccaatt ggactcacaa gtggagttca accggagaca
     1921 ctccgtagct gtaagctttg cgctgaaagc gctggcaagg ttgacagaca catggaagtg
     1981 cctgcatcga gggagcccga aaatcccatg tgagatgtgg aaggcaatcc aggcctgctg
     2041 catgttacat aacatagtga tagacatgga ggaggcaccc atggacccag ctttcttgta
     2101 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     2161 cagtcaaaat aaaatcatta tttgaggcta ggtggaggct cagtgatgat aagtctgcga
     2221 tggtggatgc atgtgtcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaga
     2281 gggcacaatc ctattccgcg ctatccgaca atctccaaga cattaggtgg agttcagttc
     2341 ggcgtatggc atatgtcgct ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2401 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2461 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2521 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2581 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2641 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2701 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2761 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2821 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2881 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2941 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3001 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctctattca
     3061 acaaagccgc cgtcccgtca agtcagcgta aatgggtagg gggcttcaaa tcgtccgctc
     3121 tgccagtgtt acaaccaatt aacaaattct gattagaaaa actcatcgag catcaaatga
     3181 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     3241 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     3301 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     3361 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta
     3421 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     3481 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
     3541 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     3601 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     3661 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     3721 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     3781 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     3841 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     3901 aaatcagcat ccatgttgga atttaatcgc ggcctcgagc aagacgtttc ccgttgaata
     3961 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
     4021 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttcc
     4081 cccgccgctc tagaactagt ggatccaaat aaaacgaaag gctcagtcga aagactgggc
     4141 ctttcgtttt atctgttgtt tgtcgcatta tacgagacgt ccaggttggg atacctga
//
