LOCUS       sequence-378714-        3379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#77.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM62
FEATURES             Location/Qualifiers
     source          1..3379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1266..1365)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1422..1438)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1422..1438)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1435..1457)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1610..2198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1690..1709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2334..3149)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2334..3149)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3057..3076
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3141..3160
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3270..3286
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3270..3286
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      3287..3333
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gaaattagac catgttctca acagttctga
      301 atcacagcaa cttgttaaaa atcataggtt atggagttgg gttggtcctg aaagaattaa
      361 aattcttttg aggaacatcg cagatggagc tttaagtact aatgctgaga ggagatatat
      421 gcataactct gtaaacacct cttgtctgag ctgcgacgcg ttggaggaga catgtctgca
      481 tgctcttcgt gattgtccaa aggtggctgc cgtttggaga agtgtgttac ctttggagtt
      541 agaagtgaag ttttttaata ttgatgaggt aacactgtgg ctggaaagaa atttatcttt
      601 ttcagttgat tgggcaacta agttcgggtt tgcagtggaa gtgatatggg aatctcgaaa
      661 cgagattgat ctgctcgatc caagggcttc ttcgcccatt tatatgatca tgatgatcat
      721 aattcgttat gaagatttta tcagagccca tccttctcac aagttaatga tgagggatca
      781 tattcagcca tgtagtagtg atcacctctt caagtggagg cccccagttg atccttggct
      841 gaagctaaac gtggatggtg ccatcgatcc ttgttctaaa actgctgcat gtggaggtat
      901 attcagagac tacagtggta gattcgtgct tgggttttca gttaagcttg atatggaata
      961 tttttgctca tttgatgaag ctgaaatttg gggtgtttat catggtatta agattgcaag
     1021 gcagtatgat tttggtaaaa tagttgtgga gtcagactcg gcgaaagcta ttcgttttgt
     1081 gcaggatggt tgtcctacat accaacagca tagtttccca ctctgtcatg agttgcgagc
     1141 tctaacgttg tctgctacaa attatttaga ctggagtctt atctataggg aagggaacat
     1201 ggcggctgct tcctttgcca atttcggctt atccatgaat cagcgagcgt ggattttttc
     1261 tgcagaccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
     1321 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgaggct aggtggaggc
     1381 tcagtgatga taagtctgcg atggtggatg catgtgtcat ggtcatagct gtttcctgtg
     1441 tgaaattgtt atccgctcag agggcacaat cctattccgc gctatccgac aatctccaag
     1501 acattaggtg gagttcagtt cggcgtatgg catatgtcgc tggaaagaac atgtgagcaa
     1561 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1621 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1681 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1741 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1801 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1861 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1921 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1981 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2041 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2101 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2161 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2221 cggggtctga cgctctattc aacaaagccg ccgtcccgtc aagtcagcgt aaatgggtag
     2281 ggggcttcaa atcgtccgct ctgccagtgt tacaaccaat taacaaattc tgattagaaa
     2341 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     2401 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     2461 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     2521 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     2581 ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta
     2641 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     2701 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     2761 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     2821 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     2881 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     2941 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     3001 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     3061 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgag
     3121 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     3181 gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
     3241 tgagacacaa cgtggctttc ccccgccgct ctagaactag tggatccaaa taaaacgaaa
     3301 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcgcatt atacgagacg
     3361 tccaggttgg gatacctga
//
