LOCUS       sequence-378715-        3415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#79.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM64
FEATURES             Location/Qualifiers
     source          1..3415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1302..1401)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1458..1474)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1458..1474)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1471..1493)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1646..2234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1726..1745)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2370..3185)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2370..3185)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3093..3112
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3177..3196
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3306..3322
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3306..3322
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      3323..3369
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gagtaaaagg aaaagggggg gtcgcccgaa
      301 aagtaaatac tggaatgaag ctgaacaact aggaaaagat acgtggaagt gcaaccattg
      361 taaagagaaa tttggtggag gtgctacaag gattgaagag catattactg gcaaaggaaa
      421 caatattaga aaatgcccca aataccgtgg caacttagga ggtcacatca ttcttgcttc
      481 cggttcctcc accagtccac aagaagctct aaatatattg aatcaattac aagaagatga
      541 aaataatttg ggagaccaga tactggagca tgatccggct gcatttgaaa gcaatgacat
      601 agaaaaggcg tttttttatc caaaggggga ccctgatgct gtttgtatca gaaagagcga
      661 tattgagctt ttacagcctc agaaatgcct taatgataat atcattgact tttatataaa
      721 gtatttgata aataaactcc caactgataa acaggacagg tttcactttt tcaattgctt
      781 tttctttcct aagcttgttg atttaagcac agataatcca tcaattgctt ctgatggtaa
      841 agcagcattt cagcgtgtaa gcaaattgac aagaaaagtg aacctttttg aaaagaatta
      901 tatcttcatt cccataaatt atagtcttca ctggagtttg attgtcattt gtcaccctgc
      961 tgaagtcatg acatgctaca gagatgaaga aactaaggga tctcccaaag aagcttgcat
     1021 cttgcacatg gattcccgaa aaggaattca tcaagatctc cacaatgttt tccaaagtta
     1081 tctatgtgaa gaatggaaag agaggcacaa caatgtgagg gatgatgttt cttctatatt
     1141 tttagatctt ccattcgtgc cacttgagct gcctcagcaa caaaatgcat acgattgtgg
     1201 catctttttg ctccactatg tggaacgttt tctggaacaa gctccaatca actttaaccg
     1261 ttccctgata agcaagttca gttattggtt ccctccacca gacccagctt tcttgtacaa
     1321 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     1381 tcaaaataaa atcattattt gaggctaggt ggaggctcag tgatgataag tctgcgatgg
     1441 tggatgcatg tgtcatggtc atagctgttt cctgtgtgaa attgttatcc gctcagaggg
     1501 cacaatccta ttccgcgcta tccgacaatc tccaagacat taggtggagt tcagttcggc
     1561 gtatggcata tgtcgctgga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1621 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1681 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1741 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1801 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1861 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1921 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1981 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2041 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     2101 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2161 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2221 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct ctattcaaca
     2281 aagccgccgt cccgtcaagt cagcgtaaat gggtaggggg cttcaaatcg tccgctctgc
     2341 cagtgttaca accaattaac aaattctgat tagaaaaact catcgagcat caaatgaaac
     2401 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     2461 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     2521 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     2581 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     2641 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     2701 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     2761 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     2821 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     2881 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     2941 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     3001 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     3061 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     3121 tcagcatcca tgttggaatt taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg
     3181 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     3241 atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttccccc
     3301 gccgctctag aactagtgga tccaaataaa acgaaaggct cagtcgaaag actgggcctt
     3361 tcgttttatc tgttgtttgt cgcattatac gagacgtcca ggttgggata cctga
//
