LOCUS       sequence-378716-        3595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM66
FEATURES             Location/Qualifiers
     source          1..3595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1482..1581)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1638..1654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1638..1654)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1651..1673)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1826..2414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1906..1925)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2550..3365)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2550..3365)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3273..3292
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3357..3376
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3486..3502
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3486..3502
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      3503..3549
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat ggatcatgtt gatggcgtta ccaatcaatt
      301 gcaagtcaac attcagaaga ctaagttgga aataaaatgt gaatacttat ttccaaaagt
      361 caacaatgat ggtattgttg atgttactcc caaagttgta atcgatctca tcgatactgt
      421 tgtagtgaat ctgggtgatt tattgaaggt ttcttgttct agttcactac tttttgttcg
      481 tggacctaac aaagagatcg gagatgtttt taaggagttg aagttattga gaaattttgt
      541 atgctatgtt tcggacagat tcatagagat gaaaagtcaa tatattgatt tcttcattca
      601 tgttttagag gtgaccaacc acgctgcaat ggttgcctgg ttgtatctcc caagaaatga
      661 caacgaaaat caggaaacaa actgtttact ttctgatcat ttgaacatga agattaagcc
      721 cattgaccca agcatccgca agatctatat tgatgtgttg caagctctac gatcagaatg
      781 gcgtccgatt attccaattg atcatgtagc taattgtgta gctgactttg tcgagactct
      841 ccggcacaac ttgaaggcaa tatcggttag taatcccaaa actcatcaaa tagcagacct
      901 tcaggagatg ctcaacctct taattgctaa tctctccatt caagatcttg aaattcacct
      961 tcaagatatc gatactgtga tgattgattc cggaatactt gtctactcgt tgtgtgaaaa
     1021 tgtggtgtta ggggaagtga ctattgattt accagttatg attgagcaca tcaagatatt
     1081 gatctaccac atcatccgga aggagttcca atctagtttg ccaaggattc atggactagg
     1141 ctatgttgat ttcgtcttaa gcaatttgaa ggagtttcaa gaccgttatc cagattcgct
     1201 tgcttttatg aagactcaac ttcaaataat tcaagcagaa ctggagagcg tccagccttt
     1261 tctgaggttt gttgcaaaaa aacaatacaa tattcacaac gaacttcaga atagtgtggc
     1321 tctactgatt ggcaaagcat atgaggtaga atatatagtt gatgcttgtg tgagcaaaag
     1381 agttccggac tggtgtctca tgctttggct cttggacatt agcacagagg ttgcagagat
     1441 gcaacaaaag aaaatgtttg aggttgactt agtgtcacca gacccagctt tcttgtacaa
     1501 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     1561 tcaaaataaa atcattattt gaggctaggt ggaggctcag tgatgataag tctgcgatgg
     1621 tggatgcatg tgtcatggtc atagctgttt cctgtgtgaa attgttatcc gctcagaggg
     1681 cacaatccta ttccgcgcta tccgacaatc tccaagacat taggtggagt tcagttcggc
     1741 gtatggcata tgtcgctgga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1801 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1861 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1921 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1981 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2041 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2101 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2161 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2221 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     2281 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2341 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2401 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct ctattcaaca
     2461 aagccgccgt cccgtcaagt cagcgtaaat gggtaggggg cttcaaatcg tccgctctgc
     2521 cagtgttaca accaattaac aaattctgat tagaaaaact catcgagcat caaatgaaac
     2581 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     2641 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     2701 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     2761 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     2821 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     2881 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     2941 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     3001 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     3061 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     3121 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     3181 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     3241 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     3301 tcagcatcca tgttggaatt taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg
     3361 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     3421 atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttccccc
     3481 gccgctctag aactagtgga tccaaataaa acgaaaggct cagtcgaaag actgggcctt
     3541 tcgttttatc tgttgtttgt cgcattatac gagacgtcca ggttgggata cctga
//
