LOCUS       sequence-378719-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SspBmicro-mCh-p60, a recruitable katanin for microtubule 
            severing, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Meiring JCM, Grigoriev I, Nijenhuis W, Kapitein LC, Akhmanova A
  TITLE     Opto-katanin, an optogenetic tool for localized, microtubule 
            disassembly.
  JOURNAL   Curr Biol. 2022 Nov 7;32(21):4660-4674.e6. doi: 
            10.1016/j.cub.2022.09.010. Epub 2022 Sep 28.
  PUBMED    36174574
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.09.010"; journalName: "Curr Biol"; date: 
            "2022-11-7- 7"; volume: "32"; issue: "21"; pages: "4660-4674.e6"
COMMENT     Sequence Label: SspBmicro-mCh-p60
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(89..295,296..298,299..418)
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein and with the mutation R74Q to reduce the 
                     affinity for iLID"
                     /label=SspB micro
                     /note="dimerizes with iLID at moderate affinity to create 
                     iLID micro (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNAQFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     primer_bind     complement(669..687)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tntcttcctt ctctgttctc cgcagccccc aagctaggca attgggcgcg ccaccatgga
       61 tctgagcggc ctgaccctgc aggaattcag ctccccgaaa cgccctaagc tgctgcgtga
      121 atattacgat tggctggttg ataacagctt taccccatat ctggtggtgg atgccacata
      181 cctgggcgtg aacgtgcccg tggagtatgt gaaagacggt cagatcgtgc tgaatctgtc
      241 tgcaagtgcg accggcaacc tgcaactgac aaatgatttt atccagttca acgcccagtt
      301 taagggcgtg tctcgtgaac tgtatatccc gatgggtgcc gctctggcca tttacgctcg
      361 cgagaacggc gatggtgtga tgttcgaacc agaagaaatc tatgacgagc tgaatattgg
      421 tcaattgtct agaggtggcg ggggcagtgg gggaggggga agtggaggcg gcggttctgg
      481 cgggggaggc tccggtggcg gggggagcgg gggtgggagt aagcttgtga gcaagggcga
      541 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      601 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      661 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      721 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
      781 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
      841 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacag
      901 gtgaagctgc gcgcacnact tcccncnacg gtcccgtatg cagaganacn tgggctggga
      961 ggctcnccna gcngnngtac cccgangnng nnnccng
//
