LOCUS       sequence-37876-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Linda Hicke lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LHP1032 - EYFP-Nterm
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..314)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(105..126)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     713..734
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggacactagt aacggccgcc agtgtgctgg aattcgccct tgcggccgcc tctgagtccg
       61 gacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
      121 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactggta gctcaggtag
      181 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
      241 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg
      301 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
      361 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
      421 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agctgccgtc gtccttgaag
      481 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
      541 ttcatgtggt cggggtagcg ggcgaagcac tgcaggccgt agccgaaggt ggtcacgagg
      601 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
      661 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
      721 tccagctcga ccaggatggg caccaccccg gtgacagctc ctcgcccttg ctcaccatgc
      781 ggccgcgttt aagggcgaat tctgcagata tccatcacac tggcggccgc tcgagcatgc
//
