LOCUS       sequence-378769-       11156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid to generate a transgenic Hydra Zic4 reporter line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11156)
  AUTHORS   Vogg MC, Ferenc J, Buzgariu WC, Perruchoud C , Papasaikas P, Sanchez
            PGL, Nuninger C , Delucinge-Vivier C , Rampon C , Beccari L, Vriz S 
            , Vincent S , Galliot B , Tsiairis CD
  TITLE     The transcription factor Zic4 acts as a transdifferentiation switch
  JOURNAL   bioRxiv 2021.12.22.47383
REFERENCE   2  (bases 1 to 11156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.12.22.47383"
COMMENT     Sequence Label: Zic4-3505:GFP
FEATURES             Location/Qualifiers
     source          1..11156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     5168..5184
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5169..5185
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9039..9755
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(9069..9097)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     9692..9712
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(10515..10534)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10516..10534)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10541..10558)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10541..10557)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10550..10572)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10699..11154
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10786..10805)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10996..11017
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga
     2221 ggtcgactct agaggatccc ccatcgatct gactaaccta accagtgcaa aaaaatttaa
     2281 aagatttgca ttgtgaaagt tagaatatta taaaaaatct aaaacgagta ttactcgagt
     2341 aaatgttata cgatctatag attaaatata ttaaaaatgt atagcgaatg ttaaactaaa
     2401 tatataatat aaacttgaaa acttactaaa ttgcaaaaac tcaaaaccga ctgtatcatt
     2461 tttacaggaa accgttattc aagatactta agttgtttac tacattatta taacatcttg
     2521 caattagcaa gacaatcgtt attttaacat cacggtatcg aaaggatttt gagaaatttt
     2581 attgaaacat tttaaacaaa aaatatcata tttagatgca ttttaagccg agatgcagga
     2641 ttctgaatga aaaagaaaaa aagaagtctc ggtagagtaa aagtgatcgg tttgcaactg
     2701 taaaatttat tgaagtacca ataattttat ttaaaataaa actgaaatat aaagttaaag
     2761 ttgctgttct ataagtttac tcgaatttta aaaccattgt aacgctagag taatatttga
     2821 gtctactaag ttagtccccg cactttttaa tcaagcaata aatacccaaa ctttgcttat
     2881 tcaaatcaat aaaccaatat atctcttaaa ataaagtaaa aacttctgaa attctataaa
     2941 aaaaaattta atttcgaaat atcaaatgta acttcaacac cgcactattt tcttttaaac
     3001 aactgatata gtaattactt ctcaaaaacg ttatctcaag gtttgtgatg tacttaaaac
     3061 cactcctatt ttgttacgcg tttaaaaaag caaacataag ttggtttcta ttgatgaatg
     3121 agaacatatt tcatttaaag ttaaaatcct accagtggtt tcactgtacg taaacaccgt
     3181 caaaaaaaca ggaacgtttt taaagattaa taattgaagt aaaaaaaatt taataccggg
     3241 ggttaaaaaa atcttttaaa ataattataa atatatatat taaaatttat aaatttttaa
     3301 acacatttaa aatatatatt aagtataata aaagtaatat tataaaaaaa aatttaattt
     3361 tataattatt tttattaaat ttataaataa taggtaaaac ttacatatcc gttttatttt
     3421 ttcttaataa aataacgcgt gcaaattttt gtccatataa agaccttttc gaacaataac
     3481 ttttttgctt agccgttttt tttcttatat ggtcaaaaaa gcgctcaagc gattcaccat
     3541 aaaaagcgca attagttcag cgttcgttat tcagaagctt cagctttgct tgatactcag
     3601 ctcttctctt tttaaacaaa acacttaatc aaaatggccg atgatgaagt tgccgccctc
     3661 gctgcagccc cggtagaaaa agtcgacatg gttagtaaag gagaagaaga taatatggct
     3721 attattaaag aatttatgag atttaaagtt catatggaag gatctgttaa tggtcatgaa
     3781 tttgaaattg aaggtgaagg agaaggtaga ccatatgaag gtactcaaac agctaaactt
     3841 aaagttacta aaggtggacc attacctttt gcatgggata ttctttcacc acaatttatg
     3901 tatggaagta aagcttatgt taaacatcca gcagatattc ctgattatct taaactttca
     3961 tttcctgaag gttttaaatg ggaaagagtt atgaattttg aagatggtgg agttgttact
     4021 gttacacaag attcttcatt acaagatgga gaatttattt ataaagttaa acttagagga
     4081 actaattttc caagtgatgg tcctgttatg caaaagaaaa ctatgggttg ggaagctagt
     4141 tctgaaagaa tgtatcctga agatggagca cttaaaggtg aaattaaaca aagacttaaa
     4201 cttaaagatg gtggacatta tgatgctgaa gttaaaacta catataaagc taaaaaacca
     4261 gttcaattac ctggagctta taatgttaat attaaacttg atattacttc tcataatgaa
     4321 gattatacaa ttgttgaaca atatgaaaga gcagaaggta gacattcaac aggtggaatg
     4381 gatgaattat ataaataaca ttcgtagaat tcacaattcg attatattta tactggacta
     4441 tttttacatc tgttcggtta ttttcacatt tatttttcta tatatatctt ataaacgttt
     4501 taaaacccat gtaatttttg ttaagctgta atataaaaga cgtcctaaca aacttctttt
     4561 attactgaat ttcctttaat tataataaat aacaagtttt aaaataaatt caggcaatta
     4621 aggcgctcct gaggtactaa aattaatgta aacatttaaa attaacttgg atggtcttaa
     4681 gtactgtact cgtgattttg ttatacttta ttattagaaa agtcgtctat taactttttg
     4741 ttccttaatt tacttgatta aattgtcgct taatttatca aatcaggttt tgcgcgttat
     4801 tttagagaaa aacttattag aaaaatgaat aagcaaagtt taggctaaca tgttttttta
     4861 ttattttaaa tagttcaagt caatgacgta taaaatgcat ttgcaaaaaa ttttaagtaa
     4921 ccctataaac ttagcaatag tagatactgg atgcaagcat tcagtagcag cattgcatat
     4981 ctgctgtctt tacgtacaaa taacagcaaa aatggacctt tattggcttc acatcgtcgt
     5041 aaaacatgtg ttattggact tgtcacaaat gtgttaagta tacagagctt agctcttgat
     5101 gttgatcact agttctagag cggccgccac cgcggtggag ctcggcgcgc cggtaccggg
     5161 ccccccctcg aggtcgacgg tatcgataag cttgatatcc catgtcggtt gtgtcttata
     5221 gcgacttcga cttttttatt ttctccaaaa aaacttccga cataaactag actttgagta
     5281 aagtcgaaga gttgtttatt tatattcttc tttctgtttt atttgctggt aaagtttttt
     5341 caataaaaat tatcaactga ggattaactg caataaaaaa gtactatctg taaataaaat
     5401 aataataata ataaataaaa aagatggtaa aaatttactt aagtaataat aaaaaacttt
     5461 tttgttcatt aaaatatata tatatagttg ttttgtaata atttttaaat tttttacaat
     5521 acatatccct tatctctgtt aagccaaatg ttttccgtta cgaaatcctt atatattgtg
     5581 ttatttttta aagtttacgc gacaggaagc tagtaattta atatctttaa gttttttaga
     5641 ttttcaagtc aatatttctc ttttactaat aattttatga aaataatttg ttgtttttgt
     5701 tttgtcgttg agattggtac aataaaacat gaaaaaggca aatttccgat taacaaagct
     5761 ttggattttg ataaagaaaa aaaaaatcca aatttattat ttcacatgaa aggtgcgaaa
     5821 gattagcgga gttttgtttt gtttttgaaa taatacaatt acaaattctt tttagaaaat
     5881 gcggattaga atttattaaa atgacattaa cactgttttg agtctaagaa acgtaaaata
     5941 tgctagttat ttttacaaca agcaggtaaa acaataaaaa tggcctttaa agtaattttg
     6001 agtttatttc attgttgtta gctcaatcga tactagttca gtaaaatacg tttatttaaa
     6061 gtattttact aaaattatgc tctttaatat tcgaaaaaag aaaacaaact ttttccaaaa
     6121 atctaacatt tttcattact caaaaaaaaa ctttcttttt gcgctttacg cagcgctaca
     6181 aaaaatatgt cgctaagtac taaaattaaa aaaaacaaaa aaacaacgta attacataac
     6241 atgacgtaat gcaactttaa tacgcatttg cagtgaatac tttattattt atatatatat
     6301 atatatactc acacatacgt atacatatat atgtataatc aaatacgtaa atagatatag
     6361 atataatata tgaatatatt ataagtttaa tttattctat tgttattgat ttttaaaaga
     6421 aagtcagaaa atcttaaact gaaagcaaaa aaaaaacttt ttcaataata tacttttatc
     6481 attattttgt tgttgttgtt atgattgtta ttataattat aataataact tttattattg
     6541 ttattattat tattattgta atcgtttatg aaaacttgat ggttgctaaa gatttaaggt
     6601 aaataaatcg ccccctctcg ctattgattt gtttatttta aataacgggc attggcgtga
     6661 aattaatggc agcttatcat acgaattgct atacattcaa agaatttttg ttagtaccca
     6721 tgagtttatt ttgaggtgac ccatgctgtt ctcattaatc taaaaccatt ggttttttga
     6781 ttaaaaggat catcgctcaa ataaagtgtt ttactcaggc gtgtgataac tgtattacta
     6841 agacttcctc gatataaact tgcatcaaat tatttctgaa taatgattat tgtcaaaaac
     6901 aaagacacag ttgactaaat tcctaactta ctttgtatta ttttttgtct gtaattttat
     6961 agaccgatat gttacttttg ctacatgtgt tagaagttgt ttgagaattc aaactgagtg
     7021 aaaaaaatgg acaaataaaa caaaataaaa cgcgtatgtc ctgctacctt tttatagctg
     7081 ttttaaataa agtttctttt ggtattctaa tgttgtgact tctaaaaaaa ttatgcaatc
     7141 aaagattatc gacgcaacga taagtcaatt aaaggaaagg ggtgctacat atgagacaac
     7201 acatgtgtgt tttacatttt taaccggttt aacgtcactc cgaacaaagt ttaaattaat
     7261 gcgaacgttt tatttttgta gctaaacatc tgagttaaaa atgcttgcgc acgttctttt
     7321 aacaaaaaca gctcatctta ttgttttact ttttattttt tattaaatta aaaaaaatat
     7381 aattaaaaaa tatttttgta agaatcttaa cagatatttt acgataaaat ttcttatcta
     7441 aagtatcgtt ttcaggttac tcgcgtagac ttataatttt caataaagat tacccaattt
     7501 gtctaattag cataacaatt aattataagt aaataaccgg agataataag taaataacct
     7561 gagataaaaa cctcaaagag tacagcagat tggaaatcat tttttttttt ttcaagaaac
     7621 cgcgcgcatt cactcgtata ccttaattgt ataatctttt agttaaacaa gcaccaaaac
     7681 cggaacaaag gtagtagtat acaatcaaag caagctttga tatctctaat atttttttca
     7741 attttttttt tatccactta tttgtctgag ctatttcttt accttaatta cgctataaaa
     7801 ttttcttctc gacaagcggc gtagctttga gtttatagct cttttctgaa gtctaattcc
     7861 ttctgttttt attacaatcc tgactaagct tcctcaaagt caaggaaaac aaatgcagtt
     7921 aaaaagaaaa taatccttgt aaacacgttt catccttcat aaaaaacgta cgctaataaa
     7981 gcttttttgt ttacagttaa gcaagctaat ttagaatttc agaattactc tttgcaaaaa
     8041 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gtaataataa taattagcca
     8101 cgtcattttg caaccagatt aaaatccagg aaatctcaat gaaattattt gggaggaagt
     8161 tgtatatttc taaaatttag cagaacttat attagcgtat atcttcgaca cacataaact
     8221 tactgtgata ttacaaaatg actcaattga gttagaaata aaatcaaata aataaactaa
     8281 attttcccaa tactacaaag caggatctga aaggttcttt taaggattct cgcttcatac
     8341 tttttttgtt ttgtattatt ttgctcccgt taattaggaa ataaactacc atgcagatta
     8401 attatgggta taattgaaag ttcgtgcagt tttattcttc gtcaaattaa atcaataatt
     8461 tagttctatt tcgttttatt actataaatc ctctaaagaa catttttgct tgttttcttt
     8521 ttaaagatct cattttaatt tttaataccc cgctatgatg tgattggatg ggatcaaatg
     8581 tgattggtta aaaaagtttg aataagtatc aagaactgtc aatcaaaata aatgagcggc
     8641 caatcaggtt agttataaat ccttattagc aacataatcc ctacacatag acagatgtac
     8701 catacataat ggaaaggtaa aattgatttg tgcccaaaag catttaaaag tctcaattta
     8761 catccgaatg acaccgtatt tcagtcacag tcagttggaa gcagcgttct agacgtatca
     8821 gagggttatc tagttgataa ttgaatgtga cattgtttta ttaaatataa aagtatattt
     8881 ttatgcagcg tttgtattgt ggtgttacct cattaaatat ctacaaattg ttcctatatt
     8941 aaaacctaat tatttatcta gaaacttatt tattaaatat ctgtttcatt aaaatggagc
     9001 atcgtttaca aagttcaaca ttaggacccg gggaaaaaat gagtaaagga gaagaacttt
     9061 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
     9121 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
     9181 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc tgttatggtg
     9241 ttcaatgctt ttcaagatac ccagatcata tgaaacggca tgactttttc aagagtgcca
     9301 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
     9361 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
     9421 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
     9481 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
     9541 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
     9601 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
     9661 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
     9721 ggattacaca tggcatggat gaactataca aatagcattc gtagaattca caattcgatt
     9781 atatttatac tggactattt ttacatctgt tcggttattt tcacatttat ttttctatat
     9841 atatcttata aacgttttaa aacccatgta atttttgtta agctgtaata taaaagacgt
     9901 cctaacaaac ttcttttatt actgaatttc ctttaattat aataaataac aagttttaaa
     9961 ataaattcag gcaattaagg cgctcctgag gtactaaaat taatgtaaac atttaaaatt
    10021 aacttggatg gtcttaagta ctgtactcgt gattttgtta tactttatta ttagaaaagt
    10081 cgtctattaa ctttttgttc cttaatttac ttgattaaat tgtcgcttaa tttatcaaat
    10141 caggttttgc gcgttatttt agagaaaaac ttattagaaa aatgaataag caaagtttag
    10201 gctaacatgt ttttttatta ttttaaatag ttcaagtcaa tgacgtataa aatgcatttg
    10261 caaaaaattt taagtaaccc tataaactta gcaatagtag atactggatg caagcattca
    10321 gtagcagcat tgcatatctg ctgtctttac gtacaaataa cagcaaaaat ggacctttat
    10381 tggcttcaca tcgtcgtaaa acatgtgtta ttggacttgt cacaaatgtg ttaagtatac
    10441 agagcttagc tcttgatgtt gatcactaga gcggccgcca ccgcggtgga gctcggcgcg
    10501 ccagctccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
    10561 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
    10621 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
    10681 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
    10741 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
    10801 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
    10861 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
    10921 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
    10981 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
    11041 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
    11101 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttag
//
