LOCUS       sequence-378776-        6229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  coexpression of Cre and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6229)
  TITLE     Oka Lab Plasmids 2022
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AV0.1-CWS-Cre-mCherry
FEATURES             Location/Qualifiers
     source          1..6229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        341..644
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        645..848
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     799..819
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             898..918
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1960..2013
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2014..2724
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2159..2177)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2427..2446
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2749..3337
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2802..2822)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3220..3231)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3358..3439
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3395..3414)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3449..3468
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   3492..3632
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3492..3621
                     /label=AAV2 ITR
     rep_origin      3707..4162
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3794..3813)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4004..4025
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4179..4198)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4298..4320
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4358..4376)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4444..4548
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4549..4617,4618..5409)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4767..4786)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5580..6168
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6069..6088
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgtgg agtcgtgacg tgaattacgt catagggtta gggaggtcct
      181 gtattagagg tcacgtgagt gttttgcgac attttgcgac accatgtggt cacgctgggt
      241 atttaagccc gagtgagcac gcagggtctc catacgcgcc tagttattaa tagtaatcaa
      301 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      361 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      421 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      481 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      541 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      601 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      661 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      721 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      781 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      841 gcagagctgg tttagtgaac cgtcagatcc gctagcaagc ttggatccac catggtgccc
      901 aagaagaaga ggaaagtctc caacctgctg actgtgcacc aaaacctgcc tgccctccct
      961 gtggatgcca cctctgatga agtcaggaag aacctgatgg acatgttcag ggacaggcag
     1021 gccttctctg aacacacctg gaagatgctc ctgtctgtgt gcagatcctg ggctgcctgg
     1081 tgcaagctga acaacaggaa atggttccct gctgaacctg aggatgtgag ggactacctc
     1141 ctgtacctgc aagccagagg cctggctgtg aagaccatcc aacagcacct gggccagctc
     1201 aacatgctgc acaggagatc tggcctgcct cgcccttctg actccaatgc tgtgtccctg
     1261 gtgatgagga gaatcagaaa ggagaatgtg gatgctgggg agagagccaa gcaggccctg
     1321 gcctttgaac gcactgactt tgaccaagtc agatccctga tggagaactc tgacagatgc
     1381 caggacatca ggaacctggc cttcctgggc attgcctaca acaccctgct gcgcattgcc
     1441 gaaattgcca gaatcagagt gaaggacatc tcccgcaccg atggtgggag aatgctgatc
     1501 cacattggca ggaccaagac cctggtgtcc acagctggtg tggagaaggc cctgtccctg
     1561 ggggttacca agctggtgga gagatggatc tctgtgtctg gtgtggccga tgaccccaac
     1621 aactacctgt tctgccgggt cagaaagaat ggtgtggctg ccccttctgc cacctcccaa
     1681 ctgtccaccc gggccctgga agggatcttt gaggccaccc accgcctgat ctatggtgcc
     1741 aaggatgact ctgggcagag atacctggcc tggtctggcc actctgccag agtgggtgct
     1801 gccagggaca tggccagggc tggtgtgtcc atccctgaaa tcatgcaggc tggtggctgg
     1861 accaatgtga acattgtgat gaactacatc agaaacctgg actctgagac tggggccatg
     1921 gtgaggctgc tcgaggatgg ggacaagctt ggaagcggag agggcagagg aagtctgcta
     1981 acatgcggtg acgtcgagga gaatcctgga cctatggtga gcaagggcga ggaggataac
     2041 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     2101 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     2161 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     2221 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     2281 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     2341 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     2401 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     2461 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     2521 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     2581 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     2641 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     2701 ggcatggacg agctgtacaa gtaataagcg gccgcgaatt ctatcgataa tcaacctctg
     2761 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2821 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     2881 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     2941 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3001 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3061 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3121 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     3181 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3241 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3301 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataagtaag tgactagaac
     3361 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3421 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatctg cacgcgtggc
     3481 gcgtcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     3541 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     3601 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
     3661 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     3721 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3781 gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3841 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     3901 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     3961 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4021 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
     4081 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4141 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     4201 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     4261 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     4321 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     4381 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     4441 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4501 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4561 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4621 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4681 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4741 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4801 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4861 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4921 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4981 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5041 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5101 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5161 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5221 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5281 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5341 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5401 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5461 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5521 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5581 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5641 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5701 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5761 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5821 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5881 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5941 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6001 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6061 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6121 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6181 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
