LOCUS       sequence-378778-        9246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged BRD protein, which can be used to be expressed in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
            Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
  TITLE     Screen identifies bromodomain protein ZMYND8 in chromatin 
            recognition of transcription-associated DNA damage that promotes 
            homologous recombination.
  JOURNAL   Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
  PUBMED    25593309
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.252189"; journalName: "Genes Dev"; date: "2015-01-15- 
            15"; volume: "29"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: TRIM33-GFP
FEATURES             Location/Qualifiers
     source          1..9246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    681..705
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2581..2592)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4165..4189)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4203..4205,4206..4208,4209..4922)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4248..4269)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4509..4528)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4856..4877
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4950..4969)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4994..5042
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5244..5672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5331..5350)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5541..5562
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5681..5701)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5686..6015
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5866..6001
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5928..5947
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6063..6110
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6129..6527
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6685..6806
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6722..6741)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6776..6795
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6855..6871)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6855..6871)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6868..6890)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6879..6895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6903..6909,6910..6927,6928..6933))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6948..6969
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7086..7103)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7257..7845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7337..7356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8016..8807,8808..8876))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8639..8658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8877..8981)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9056..9075)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagttaag ctgagcatca acaagtttgt acaaaaaagc aggcttcgaa ggagatagaa
      721 ccaattctct aaggaaatac ttaaccatgg tcgactggat ccttatggcg gaaaacaaag
      781 gcggcggcga ggctgagagc ggcggcgggg gcagcggcag cgcgccggta actgccgggg
      841 ccgccgggcc cgccgcgcag gaggcggagc cgcctctcac cgcggtgctg gtggaggagg
      901 aggaggagga aggcggcagg gccggcgctg agggcggcgc ggccgggccc gacgacgggg
      961 gggtggccgc ggcctcctcg ggctcggccc aggctgcttc atctcctgcg gcctcagtgg
     1021 gcactggagt tgccgggggc gcagtatcga cgccggctcc agctccagcc tcggctcccg
     1081 ctccgggtcc ctcggcaggg ccgcctcctg gaccgccagc ctcgctcctg gacacctgcg
     1141 ccgtgtgtca gcagagcttg cagagccggc gtgaggcgga gcccaagctg ctgccctgtc
     1201 ttcactcctt ctgcctgcgc tgcctgcccg agccggagcg ccagctcagc gtgcccatcc
     1261 cggggggcag caacggcgac atccagcaag ttggtgtaat acggtgccca gtatgccgcc
     1321 aagaatgcag acagatagac cttgtggata attattttgt gaaagacaca tctgaagctc
     1381 ctagcagttc tgatgaaaaa tcagaacagg tatgtactag ttgtgaagac aatgcaagtg
     1441 cagttggctt ttgtgtagaa tgtggagagt ggctatgtaa gacatgtatc gaagcacatc
     1501 aaagagtaaa atttactaaa gatcacttga tcaggaagaa agaagatgtc tcagagtctg
     1561 ttggagcatc tggtcaacgc cctgttttct gccctgtaca caaacaagaa cagttgaaac
     1621 ttttctgtga aacatgtgat agattgacat gtagagactg tcagctattg gaacacaaag
     1681 aacataggta tcagtttttg gaagaagctt ttcaaaatca gaagggtgca attgagaatc
     1741 tactggcgaa acttcttgag aagaagaatt atgttcattt tgcagctact caggtgcaga
     1801 ataggataaa agaagtaaat gagactaaca aacgagtaga acaggaaatt aaagtggcca
     1861 ttttcaccct tatcaatgaa attaataaga aaggaaaatc tctcttacaa cagctagaga
     1921 atgttacaaa ggaaagacag atgaagttac tacagcagca gaatgacatc acaggccttt
     1981 cccggcaggt gaagcatgtt atgaacttca caaattgggc aattgcaagt ggcagcagca
     2041 cagcactact atacagcaag cgactgatta ctttccagtt gcgtcatatt ttgaaagcac
     2101 ggtgtgatcc tgtccctgct gctaatggag caatacgttt ccattgtgat cccaccttct
     2161 gggcaaagaa tgtagtcaat ttaggtaatc tagtaataga gagtaaacca gctcctggtt
     2221 atactcctaa tgttgtagtt gggcaagttc ctccagggac aaaccacatt agtaaaaccc
     2281 ctggacagat taacttagca cagcttcgac tccagcacat gcaacaacaa gtatatgcac
     2341 agaaacatca gcagttgcaa cagatgagga tgcagcaacc accagcacct gtaccaacta
     2401 caacaacaac aacacaacag catcctagac aagcagcccc tcagatgtta caacaacagc
     2461 ctcctcgatt gatcagtgtg caaacaatgc aaagaggcaa catgaactgt ggagcttttc
     2521 aagcccatca gatgagactg gctcagaatg ctgccagaat accagggata cccaggcaca
     2581 gcggccctca atattccatg atgcagccac acctccaaag acaacactca aacccagggc
     2641 atgctggacc ctttcccgta gtatcggtac acaacaccac aatcaaccca acgagcccta
     2701 ctacagcaac tatggcaaat gcaaaccgag gtcccaccag cccatctgtt acagcaatag
     2761 agctaatccc ctcagttacc aatccagaaa accttccatc gctgccagat attccaccca
     2821 tacagttgga agatgctggc tcaagtagtt tagataatct actaagtaga tacatctcag
     2881 gcagtcacct acccccacag cctacaagca ccatgaatcc ttctccaggt ccctctgccc
     2941 tttctccggg atcatcaggt ttatccaatt ctcacacacc tgtgagaccc ccaagtactt
     3001 ctagtactgg cagtcgaggc agctgtgggt catcaggaag aactgctgag aagacaagtc
     3061 ttagtttcaa atctgatcag gtgaaggtca agcaagaacc tgggactgaa gatgaaatat
     3121 gtagcttttc aggaggtgta aaacaagaaa aaacagagga tggcaggagg agtgcttgca
     3181 tgttgagcag tcctgagagt agcttgacac cacctctctc aaccaacctg catctagaaa
     3241 gtgaattgga tgcattggca agcctggaaa accatgtgaa aactgaacct gcagatatga
     3301 atgaaagctg caaacagtca gggctcagca gccttgttaa tggaaagtcc ccaattcgaa
     3361 gcctcatgca caggtcggca aggattggag gagatggcaa caataaagat gatgacccaa
     3421 atgaagactg gtgtgctgtc tgccaaaacg gaggagatct cttgtgctgc gaaaaatgtc
     3481 caaaggtctt tcatctaact tgtcatgttc caacactact tagctttcca agtggggact
     3541 ggatatgcac attttgtaga gatattggaa agccagaagt tgaatatgat tgtgataatt
     3601 tgcaacatag taagaagggg aaaactgcgc aggggttaag ccccgtggac caaaggaaat
     3661 gtgaacgtct tctgctttac ctctattgcc atgaattaag tattgaattc caggagcctg
     3721 ttcctgcttc gataccaaac tactataaaa ttataaagaa accaatggat ttatccaccg
     3781 tgaaaaagaa gcttcagaaa aaacattccc aacactacca aatcccggat gactttgtgg
     3841 ccgatgtccg tttgatcttc aagaactgtg aaaggtttaa tgaaatgatg aaagttgttc
     3901 aagtttatgc agacacacaa gagattaatt tgaaggctga ttcagaagta gctcaggcag
     3961 ggaaagcagt tgcattgtac tttgaagata aactcacaga gatctactca gacaggacct
     4021 tcgcaccttt gccagagttt gagcaggaag aggatgatgg tgaggtaact gaggactctg
     4081 atgaagactt tatacagccc cgcagaaaac gcctaaagtc agatgagaga ccagtacata
     4141 taaagttact ctcgagatat ctagacccag ctttcttgta caaagtggtt gatgctgtta
     4201 acatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4261 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4321 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     4381 ccctcgtgac caccttcacc tacggcgtgc agtgcttcgc ccgctacccc gaccacatga
     4441 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     4501 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     4561 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     4621 acaagctgga gtacaactac aacagccaca aggtctatat caccgccgac aagcagaaga
     4681 acggcatcaa ggtgaacttc aagacccgcc acaacatcga ggacggcagc gtgcagctcg
     4741 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     4801 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4861 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     4921 aatgataagt ttaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga
     4981 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
     5041 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     5101 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     5161 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag atctgcgcag
     5221 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5281 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5341 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5401 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5461 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5521 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5581 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5641 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     5701 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5761 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5821 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5881 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5941 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6001 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     6061 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     6121 actaaaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     6181 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     6241 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     6301 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     6361 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     6421 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     6481 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagca cttcgtggcc
     6541 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     6601 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     6661 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     6721 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6781 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     6841 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6901 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6961 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7021 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7081 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7141 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7201 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7261 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7321 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7381 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7441 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7501 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7561 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7621 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7681 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7741 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7801 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7861 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7921 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7981 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8041 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8101 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8161 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8221 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8281 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8341 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8401 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8461 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8521 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8581 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8641 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8701 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8761 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8821 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8881 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8941 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9001 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     9061 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     9121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     9181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     9241 cgcgtt
//
