LOCUS       sequence-37878-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the reporter gene under the control of the 
            MiniPromoter in this plasmid has not been tested.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1214
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(666..684)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        763..867
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 accatggtgg cggaagttcc tatacctttt gaagaatagg aacttcggaa taggaacttc
       61 ggcgcgccgg gcacactcag ggtcgcctga gccgctgcct gatccctggc caccgtccgt
      121 ctgaggtgtc cggcgctccc agccaccttt caagccctcg ggtgtgacca atccgcggcc
      181 aagccgagac ccttgcagcc aatcgctagg cggctgggat ttcaggggcg ggcgcgactt
      241 gccctcagct ccccacctcc ccgtcccggg gcgacagtga cataagcaaa tctgggcaga
      301 gaggtggaag tcaggtccct gtggacgtgg gaggcggtcc cttcctcttt tggctccaaa
      361 gtgcgaaagg aggcagacac agcggtctcc attacagcca gggcaagggg gctaatagct
      421 ccctagaact gatggaagtc tccattctgg gaagcatttc ttattcctat cgaggtttta
      481 aaaacacctg gcgatggtcc tctggaaatt gagggtttaa aatttcagca agagctgaag
      541 ttaaacttcc agaaacattt actatatata ggtttagtgc ttaagaaaca aagaggccgg
      601 cctaggagct cgaattcatt aagggttatt gaatatgatc ggaattaatt cttgaagacg
      661 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      721 gacgtgcggc cgccgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      781 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      841 cttcaataat attgaaaaag gaagagtatg agtattcaa
//
