LOCUS       sequence-378784-        4533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human W279A long-form UBASH3A in Gateway pDONR221 (for N-terminal 
            tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4533)
  TITLE     Concannon Lab UBASH3A Plasmids 2022
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDONR221-W279A-long-UBASH3A-Ntag
FEATURES             Location/Qualifiers
     source          1..4533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(130..157)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(249..335)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     384..406
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     398..415
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     399..415
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    431..530
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2520..2619)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2636..2655)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2637..2655)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2660..2676)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2660..2676)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2778..2797)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2789..3598
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2856..2875)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3745..4333
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4234..4253
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4487..4504
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
       61 gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt
      121 agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt
      181 tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct
      241 cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
      301 gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc
      361 gttaacgcta gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa
      421 gctcgggccc caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
      481 tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagcaggct tcatggcagc
      541 gggggagacg cagctctacg ccaaggtctc caacaagctc aagagccgca gcagcccctc
      601 gctcctggag cccctcctgg ccatgggctt cccggtgcac accgcgctga aagcgttggc
      661 agccacgggg aggaagacgg cggaggaggc cttggcctgg ctgcatgatc attgcaatga
      721 cccttcccta gacgacccca tcccccagga gtatgccctt ttcctctgtc caacggggcc
      781 cctgctggaa aaacttcaag agttctggag agagagcaag cgccagtgtg caaagaacag
      841 agctcatgag gtcttcccac acgtgacact ctgtgacttc ttcacgtgtg aagaccagaa
      901 ggtggaatgc ctgtacgagg cgctgaagag agctggagac aggctcctgg gctccttccc
      961 cacggccgtg cctctggctc tccactcctc catcagctac ctcggcttct tcgtcagtgg
     1021 cagccccgca gacgtcatcc gggaattcgc catgaccttc gccacggaag catctctctt
     1081 agcaggcact tccgtttccc gcttctggat tttcagccag gtgcctggac atggccctaa
     1141 cctgaggctg agcaatttaa ctagagcctc cttcgtgagc cactacatcc ttcaaaaata
     1201 ctgctccgtg aagccttgca ccaaacagct gcatctgacc ttggcccaca agttctaccc
     1261 ccaccaccag aggacgctgg agcagctggc cagagccatc cccctgggcc acagctgcca
     1321 gtggaccgca gcactctact cccgagacat gcgctttgtg cactaccaga ccctgagagc
     1381 cctattccag tacaaacccc agaacgtgga tgagctgacg ctaagtcctg gtgactacat
     1441 ctttgtggac cccacgcagc aggacgaagc cagcgagggc gcggtgattg ggatctcaca
     1501 gcggacgggc tgccggggct tcctgccgga aaactacacg gatcgagcca gtgagtctga
     1561 cacgtgggtg aagcacagga tgtacacctt cagtctagcc acagacctga actccagaaa
     1621 ggatggtgaa gccagcagca gatgcagcgg ggaatttctt ccacaaacgg caaggagtct
     1681 tagcagctta caggccttgc aggctaccgt tgcaaggaag agtgtgctgg tggttcgcca
     1741 cggggagaga gtggatcaga tcttcgggaa ggcatggctg cagcaatgct ccactcctga
     1801 tgggaaatac tacaggccag acctgaattt cccctgcagt ctgcccaggc ggagtcgtgg
     1861 gatcaaagac tttgaaaacg atcccccatt atcatcgtgt ggcattttcc agtccagaat
     1921 tgcaggggac gcgctactgg acagtggtat cagaatcagc tctgtgtttg cctccccagc
     1981 cctccgctgt gtgcagacgg ccaaactcat cctggaagaa ctcaaactgg agaaaaaaat
     2041 caagatacga gtggaacctg gaatctttga atggacaaaa tgggaagctg gcaaaaccac
     2101 cccaaccctc atgagcctgg aagagctgaa agaggcaaat ttcaacattg acactgatta
     2161 caggcccgcg tttcccctgt ccgccctcat gccggccgag agctaccagg agtacatgga
     2221 caggtgcacg gcgagcatgg tgcaaatcgt caacacctgc ccgcaggaca cgggtgtcat
     2281 cctaattgtg agtcacggct ccactctgga ctcctgcacg cggccactgc tcgggctgcc
     2341 gccccgggaa tgtggggatt ttgcccaact cgtgagaaag atcccttccc tgggcatgtg
     2401 cttctgtgaa gaaaataaag aggaaggaaa atgggagttg gtgaacccac cggtgaagac
     2461 cctgacccac ggggcgaacg cagcatttaa ctggaggaac tggatctcag gcaactgaaa
     2521 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     2581 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     2641 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     2701 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
     2761 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
     2821 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     2881 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     2941 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     3001 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     3061 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     3121 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     3181 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     3241 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     3301 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     3361 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     3421 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     3481 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     3541 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     3601 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc
     3661 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     3721 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     3781 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3841 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3901 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3961 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4021 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4081 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4141 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4201 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4261 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4321 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4381 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     4441 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     4501 cgaggaagcg gaagagcgcc caatacgcaa acc
//
