LOCUS       sequence-378785-        4530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human wild-type long-form UBASH3A in Gateway pDONR221 (for 
            C-terminal tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4530)
  TITLE     Concannon Lab UBASH3A Plasmids 2022
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDONR221-WT-long-UBASH3A-Ctag
FEATURES             Location/Qualifiers
     source          1..4530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2065..2164)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2181..2200)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2182..2200)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2205..2221)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2205..2221)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2323..2342)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2334..3143
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2401..2420)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3290..3878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3779..3798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4032..4049
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4208..4235)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4327..4413)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4462..4484
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4476..4493
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4477..4493
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(4509..4530,1..78)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
       61 tgtacaaaaa agcaggcttc atggcagcgg gggagacgca gctctacgcc aaggtctcca
      121 acaagctcaa gagccgcagc agcccctcgc ttctggagcc cctcctggcc atgggcttcc
      181 cggtgcacac cgcgctgaaa gcgttggcag ccacggggag gaagacggcg gaggaggcct
      241 tggcctggct gcatgatcat tgcaatgacc cttccctaga cgaccccatc ccccaggagt
      301 atgccctttt cctctgtcca acggggcccc tgctggaaaa acttcaagag ttctggagag
      361 agagcaagcg ccagtgtgca aagaacagag ctcatgaggt cttcccacac gtgacactct
      421 gtgacttctt cacgtgtgaa gaccagaagg tggaatgcct gtacgaggcg ctgaagagag
      481 ctggagacag gctcctgggc tccttcccca cggccgtgcc tctggctctc cactcctcca
      541 tcagctacct cggcttcttc gtcagtggca gccccgcaga cgtcatccgg gaattcgcca
      601 tgaccttcgc cacggaagca tctctcttag caggcacttc cgtttcccgc ttctggattt
      661 tcagccaggt gcctggacat ggccctaacc tgaggctgag caatttaact agagcctcct
      721 tcgtgagcca ctacatcctt caaaaatact gctccgtgaa gccttgcacc aaacagctgc
      781 atctgacctt ggcccacaag ttctaccccc accaccagag gacgctggag cagctggcca
      841 gagccatccc cctgggccac agctgccagt ggaccgcagc actctactcc cgagacatgc
      901 gctttgtgca ctaccagacc ctgagagccc tattccagta caaaccccag aacgtggatg
      961 agctgacgct aagtcctggt gactacatct ttgtggaccc cacgcagcag gacgaagcca
     1021 gcgagggctg ggtgattggg atctcacagc ggacgggctg ccggggcttc ctgccggaaa
     1081 actacacgga tcgagccagt gagtctgaca cgtgggtgaa gcacaggatg tacaccttca
     1141 gtctagccac agacctgaac tccagaaagg atggtgaagc cagcagcaga tgcagcgggg
     1201 aatttcttcc acaaacggca aggagtctta gcagcttaca ggccttgcag gctaccgttg
     1261 caaggaagag tgtgctggtg gttcgccacg gggagagagt ggatcagatc ttcgggaagg
     1321 catggctgca gcaatgctcc actcctgatg ggaaatacta caggccagac ctgaatttcc
     1381 cctgcagtct gcccaggcgg agtcgtggga tcaaagactt tgaaaacgat cccccattat
     1441 catcgtgtgg cattttccag tccagaattg caggggacgc gctactggac agtggtatca
     1501 gaatcagctc tgtgtttgcc tccccagccc tccgctgtgt gcagacggcc aaactcatcc
     1561 tggaagaact caaactggag aaaaaaatca agatacgagt ggaacctgga atctttgaat
     1621 ggacaaaatg ggaagctggc aaaaccaccc caaccctcat gagcctggaa gagctgaaag
     1681 aggcaaattt caacattgac actgattaca ggcccgcgtt tcccctgtcc gccctcatgc
     1741 cggccgagag ctaccaggag tacatggaca ggtgcacggc gagcatggtg caaatcgtca
     1801 acacctgccc gcaggacacg ggtgtcatcc taattgtgag tcacggctcc actctggact
     1861 cctgcacgcg gccactgctc gggctgccgc cccgggaatg tggggatttt gcccaactcg
     1921 tgagaaagat cccttccctg ggcatgtgct tctgtgaaga aaataaagag gaaggaaaat
     1981 gggagttggt gaacccaccg gtgaagaccc tgacccacgg ggcgaacgca gcatttaact
     2041 ggaggaactg gatctcaggc aacaacccag ctttcttgta caaagttggc attataagaa
     2101 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     2161 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     2221 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     2281 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     2341 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
     2401 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
     2461 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     2521 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     2581 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
     2641 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     2701 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     2761 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     2821 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
     2881 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     2941 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     3001 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     3061 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     3121 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
     3181 ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg
     3241 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3301 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3361 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3421 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3481 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3541 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3601 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3661 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3721 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3781 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3841 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3901 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3961 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     4021 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     4081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     4141 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     4201 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     4261 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     4321 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     4381 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt
     4441 taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc agtcttaagc
     4501 tcgggcccca aataatgatt ttattttgac
//
