LOCUS       sequence-378789-       11620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged BRD protein, which can be used to be expressed in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11620)
  AUTHORS   Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
            Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
  TITLE     Screen identifies bromodomain protein ZMYND8 in chromatin 
            recognition of transcription-associated DNA damage that promotes 
            homologous recombination.
  JOURNAL   Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
  PUBMED    25593309
REFERENCE   2  (bases 1 to 11620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.252189"; journalName: "Genes Dev"; date: "2015-01-15- 
            15"; volume: "29"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: GFP-BAZ2A
FEATURES             Location/Qualifiers
     source          1..11620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(709..711,712..714,715..1425)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(754..775)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1015..1034)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1362..1383
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1441..1465
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2509..2520)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7211..7235)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7243..7284
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(7324..7343)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7368..7416
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7618..8046
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7705..7724)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7915..7936
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8055..8075)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8060..8389
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8240..8375
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8302..8321
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8437..8484
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8503..8901
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    9059..9180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9096..9115)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9150..9169
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9229..9245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9229..9245)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9242..9264)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9253..9269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9277..9283,9284..9301,9302..9307))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9322..9343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9460..9477)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9631..10219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9711..9730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10390..11181,11182..11250))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11013..11032
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11251..11355)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11430..11449)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gactctagag gatccctacc ggtgatatcc tcgagaccat ggtgagcaag
      721 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      781 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      841 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      901 ttcacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
      961 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1021 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1081 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1141 aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg catcaaggtg
     1201 aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1261 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1321 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1381 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggctc gagcccatca
     1441 acaagtttgt acaaaaaagc agaagggccg tcaaggccca ccatggaaat ggaggcaaac
     1501 gaccatttta actttactgg ccttccccct gcacctgctg cctcaggact gaaaccctct
     1561 ccttcctcag gggagggcct ctacactaac gggtctccca tgaacttccc ccagcaaggg
     1621 aaaagtttga atggggatgt gaatgttaat ggcttatcta ctgtatctca cactactact
     1681 tcagggattt tgaactctgc tccccactcc tccagcacct cacacctcca tcaccccagc
     1741 gtggcctacg actgtctctg gaactactca cagtacccat ctgccaatcc tggcagcaac
     1801 ctcaaggacc caccccttct ctcccagttc tcggggggac aatacccact caacggcatc
     1861 cttgggggca gccggcaacc ttcatcccca agtcataaca ctaaccttcg ggctgggagc
     1921 caagagttct gggccaacgg tacccagagt cccatggggc ttaactttga ttcacaagaa
     1981 ctgtatgatt cctttcctga ccagaatttt gaggtgatgc ccaatggacc ccctagtttt
     2041 ttcacctccc cacagacttc tcctatgttg ggatctagca ttcaaacctt tgcaccctcc
     2101 caggaggtag gcagtggtat ccatcctgat gaggcagcag aaaaggagat gacttcagtt
     2161 gtggcagaga atggcactgg cttggtaggc agcttggagc tggaagaaga gcagccagaa
     2221 ctgaagatgt gtggctacaa tggctctgtc ccttctgtgg aatcgttaca ccaagaggtc
     2281 tcagtcctgg tccctgaccc cacagtgagc tgtttagatg atccttcaca tcttcctgat
     2341 caactggaag acactccaat cctcagtgaa gactctctgg agcccttcaa ctctctggca
     2401 ccagagccag tgagtggagg actatatggt attgatgaca cggagctgat gggtgcagag
     2461 gacaagctgc ctcttgagga cagccctgtg atttctgccc ttgattgccc ttccctcaat
     2521 aatgctactg ccttcagtct cctggcagat gatagtcaaa catcaacctc tatctttgcc
     2581 agtcccacct ctccacctgt cctaggggag tctgtcctgc aagataacag ctttgacctg
     2641 aataatggta gtgacgctga acaggaagaa atggaaactc aatcttcaga cttcccacca
     2701 tccctgaccc agccagctcc tgatcagtca tccactattc agctacatcc agcaacctca
     2761 ccagcagtct cgccaacaac ctccccagca gtctccctag tggtttctcc agcagcctcc
     2821 ccagaaatct ctccagaagt ttgtcccgca gcttctacag ttgtctctcc agcagtcttc
     2881 tcagtggtct ctccagcttc ctcagcagtc ctcccagcag tctccttaga agtcccgttg
     2941 acggcttcag tgacatcccc aaaagcctct cccgtaactt ccccagcagc tgcctttcca
     3001 acagcctccc cagcaaataa ggatgtcagc agctttctag aaaccactgc tgacgtggaa
     3061 gagatcactg gagaaggact cactgcttct ggtagtggtg atgtcatgag gagacgtatt
     3121 gctaccccag aagaagttcg tcttcccctc caacatgggt ggcggagaga ggtgcgcatc
     3181 aagaagggca gccaccgatg gcagggggag acctggtatt atggcccctg tgggaagagg
     3241 atgaagcaat ttccagaagt gatcaagtac ctgagccgca acgtggtaca cagtgtccgc
     3301 cgagagcact tcagcttcag tccccgtatg cctgttggag atttctttga agaaagagac
     3361 acgccagagg gcttgcagtg ggtgcagctc tcagcagagg agatcccgtc gaggattcag
     3421 gcaattactg gcaaacgggg tcgacctcga aacactgaga aggctaagac taaggaagtc
     3481 cccaaggtga aacggggtcg aggtcggcca cctaaggtca aaatcactga gctattgaac
     3541 aagacagaca accgccccct aaagaaactg gaggcccaag aaacattgaa tgaggaggat
     3601 aaagcaaaga ttgctaaaag caagaagaag atgaggcaga aggttcaacg gggagagtgt
     3661 cagactacta tccaagggca ggccagaaat aagcggaaac aagagaccaa gagcttaaag
     3721 cagaaggaag ctaagaagaa atccaaggct gagaaagaaa aaggaaagac aaagcaggaa
     3781 aaactgaagg aaaaagtcaa gagggaaaag aaggagaagg taaaaatgaa ggaaaaggag
     3841 gaggtgacca aagccaagcc agcctgtaaa gcagataaga ccctggccac acagaggcgc
     3901 ttggaggaac ggcagaggca gcagatgatc ttggaggaaa tgaagaagcc gacagaggat
     3961 atgtgtctga ctgaccacca gcccctgcct gacttctcac gagtccctgg tctgacattg
     4021 cccagtggag ccttctcaga ctgcttgacc attgtggagt tcctgcatag ctttggcaag
     4081 gtgctgggct ttgatcctgc caaagatgtg cctagcctgg gggtcctgca ggagggactc
     4141 ctgtgtcaag gtgacagctt gggtgaggtg caagacctgc tggtcaggct gctgaaggct
     4201 gcactccatg atcctggctt tccctcctac tgtcagtccc taaagatctt gggggagaag
     4261 gtgtctgaga tcccactgac aagagacaat gtgtcagaga tcctgcgctg cttccttatg
     4321 gcatatggag tagagccagc cctctgtgac cgcctgcgca cccagccttt tcaggcccag
     4381 ccaccccagc agaaggctgc tgtcctggcc ttccttgtgc atgagctcaa tggctccacc
     4441 ctcatcatca atgagattga caagactctg gagagtatgt ccagctacag gaaaaacaag
     4501 tggattgttg aaggccggct ccggaggctg aaaactgttc tggccaagcg aactgggcgg
     4561 tctgaagtag agatggaagg gccagaggaa tgcctgggac ggaggcgcag ttctcggatc
     4621 atggaggaga ccagtggcat ggaagaagag gaagaagagg agtctatagc agctgtccct
     4681 ggccgcaggg gtcgaagaga tggagaggtt gatgccacag catctagcat cccagagcta
     4741 gagcgccaga tagaaaaact cagcaagcgt cagcttttct ttcgcaaaaa gctgcttcac
     4801 tcatcccaga tgcttcgggc ggtctccctg ggtcaggacc gctacagacg tcgctactgg
     4861 gtattgccgt atttggctgg tatctttgta gaaggaacag aggggaactt agttcctgag
     4921 gaggtgataa agaaggaaac tgactcctta aaagtggcag cccatgcgtc actcaaccct
     4981 gccctcttct ctatgaagat ggagttagct ggctccaaca ccactgccag ttctcctgcc
     5041 cgggcccgag gccgacctcg aaaaactaag cccgggtcta tgcaacctag gcatcttaag
     5101 tcccctgtca ggggtcagga ttcagaacag ccccaggccc agcttcagcc tgaggctcag
     5161 cttcatgctc ctgcccagcc ccagcctcag cttcagcttc agcttcagtc ccataagggg
     5221 ttcctggagc aagaaggctc ccctttgtca ctgggtcaga gccagcatga cctcagccag
     5281 tcagccttcc tgtcttggct gagccagact cagagccata gctccctgtt gagcagctca
     5341 gtcctcacac ctgatagcag tccgggaaaa ctagacccag ctccatcaca acccccggag
     5401 gagccagagc ctgatgaggc agaatccagc cctgatcctc aagcactctg gtttaacatc
     5461 tcagcccaga tgccctgcaa tgctgccccc acaccgcccc ctgcagtttc tgaggaccaa
     5521 cccactccct cccctcagca gcttgcctcc tccaagccaa tgaatagacc tagtgctgcc
     5581 aacccttgtt ctccagtgca gttctcttcc acgcccttgg ctgggttggc ccctaagagg
     5641 cgagcaggag accctggaga aatgccacag agtcccacag ggctgggaca gcccaaacgg
     5701 agagggagac ctcccagtaa gttcttcaaa cagatggaac agcgttacct aacccagctg
     5761 acagcccagc ctgtcccacc tgagatgtgc tcaggctggt ggtggatacg agatcctgag
     5821 atgttggatg ccatgctcaa ggccctacac ccccgaggta tccgggagaa ggcacttcac
     5881 aaacacctta acaagcacag ggacttcttg caggaagtct gcctgcggcc ctcagctgac
     5941 cccatctttg agcccaggca actacctgcc tttcaagaag ggattatgag ctggtccccc
     6001 aaagagaaga catacgagac agacctagca gtgcttcaat gggtagagga gctggagcag
     6061 cgggttatca tgtctgatct gcagattcgg ggctggacat gtcctagccc agactctacc
     6121 cgtgaagact tggcctactg tgagcacctc tccgactccc aggaggatat cacctggcga
     6181 ggtcggggca gggagggact ggcacctcag cgtaaaacta ccaacccttt ggacctggct
     6241 gtgatgcggc tggctgccct ggaacagaat gtagaacggc ggtacctgcg ggagcccctc
     6301 tggccaactc atgaggttgt gctggagaag gccctgctta gcacacctaa tggtgcccct
     6361 gagggcacca ctacagagat atcatatgag atcacccctc gcattcgtgt ctggcgccag
     6421 accctcgagc ggtgccggag cgcagcccag gtgtgcttgt gcctgggcca gctggagagg
     6481 tccattgcct gggagaagtc tgtcaacaaa gtgacatgtc tagtctgccg gaagggtgac
     6541 aatgatgagt ttcttctgct ttgtgatggg tgtgaccgtg gctgccacat ttactgccat
     6601 cgtcccaaga tggaggctgt cccagaagga gattggttct gtactgtctg tttggctcag
     6661 caggtggagg gagaattcac tcagaagcct ggtttcccaa agcgtggcca gaagcggaaa
     6721 agtggttatt cgctgaactt ctcagagggt gatggccgcc gacgccgggt actgttgagg
     6781 ggccgagaaa gcccagcagc agggcctcgg tactcggaag aagggctctc cccctccaag
     6841 cggcggcgac tctctatgcg gaaccaccac agtgatctca cattttgcga gattatcctg
     6901 atggagatgg agtcccatga tgcagcctgg cctttcctag agcctgtgaa cccacgtttg
     6961 gtgagtgggt accggcgcat catcaaaaat cctatggatt tttccaccat gcgggagcgg
     7021 ctgctcaggg gagggtacac cagctcagag gagtttgcgg ctgatgccct cctggtattt
     7081 gacaactgcc agactttcaa cgaggatgac tctgaagtag gcaaggctgg gcacatcatg
     7141 cgccgcttct tcgagagccg ctgggaggag ttttatcagg gaaaacaggc caatctgtca
     7201 ggcctcatgg gcccagcttt cttgtacaaa gtggttgata acgggaagcc tatccctaac
     7261 cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagtttaaac gggggaggct
     7321 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     7381 acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     7441 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     7501 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     7561 gggcggcagg ccctgccata gcagatctgc gcagctgggg ctctaggggg tatccccacg
     7621 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7681 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7741 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7801 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7861 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7921 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     7981 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8041 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     8101 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     8161 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     8221 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8281 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     8341 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     8401 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     8461 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gcctttgtct
     8521 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     8581 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     8641 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     8701 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     8761 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     8821 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     8881 ggttatgtgt gggagggcta agcacttcgt ggccgaggag caggactgac acgtgctacg
     8941 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     9001 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     9061 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9121 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9181 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     9241 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     9301 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     9361 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     9421 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9481 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9541 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9601 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9661 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9721 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9781 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9841 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9901 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9961 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10021 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    10081 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10141 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10201 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10261 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    10321 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    10381 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10441 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10501 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10561 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10621 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10681 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10741 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10801 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    10861 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10921 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10981 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    11041 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    11101 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    11161 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    11221 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    11281 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    11341 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
    11401 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
    11461 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
    11521 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
    11581 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
//
