LOCUS       sequence-378790-        4419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human wild-type short-form UBASH3A in Gateway pDONR221 (for 
            N-terminal tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ge Y, Paisie TK, Chen S, Concannon P
  TITLE     UBASH3A Regulates the Synthesis and Dynamics of TCR-CD3 Complexes.
  JOURNAL   J Immunol. 2019 Dec 1;203(11):2827-2836. doi: 
            10.4049/jimmunol.1801338. Epub 2019 Oct 28.
  PUBMED    31659016
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1801338"; journalName: "J Immunol"; date: 
            "2019-12-1- 1"; volume: "203"; issue: "11"; pages: "2827-2836"
COMMENT     Sequence Label: pDONR221-WT-short-UBASH3A-Ntag
FEATURES             Location/Qualifiers
     source          1..4419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     112..131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     365..382
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(541..568)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(660..746)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     795..817
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     809..826
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     810..826
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    842..941
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2817..2916)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2933..2952)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2934..2952)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2957..2973)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2957..2973)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3075..3094)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3086..3895
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3153..3172)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(4042..4419,1..211)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
       61 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      121 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      181 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
      241 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
      301 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
      361 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
      421 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
      481 cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca
      541 aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg
      601 cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga
      661 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
      721 tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct
      781 agcatggatg ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc
      841 ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca
      901 atgctttttt ataatgccaa ctttgtacaa aaaagcaggc ttcatggcag cgggggagac
      961 gcagctctac gccaaggtct ccaacaagct caagagccgc agcagcccct cgctcctgga
     1021 gcccctcctg gccatgggct tcccggtgca caccgcgctg aaagcgttgg cagccacggg
     1081 gaggaagacg gcggaggagg ccttggcctg gctgcatgat cattgcaatg acccttccct
     1141 agacgacccc atcccccagg agtatgccct tttcctctgt ccaacggggc ccctgctgga
     1201 aaaacttcaa gagttctgga gagagagcaa gcgccagtgt gcaaagaaca gagctcatga
     1261 ggtcttccca cacgtgacac tctgtgactt cttcacgtgt gaagaccaga aggtggaatg
     1321 cctgtacgag gcgctgaaga gagctggaga caggctcctg ggctccttcc ccacggccgt
     1381 gcctctggct ctccactcct ccatcagcta cctcggcttc ttcgtcagtg gcagccccgc
     1441 agacgtcatc cgggaattcg ccatgacctt cgccacggaa gcatctctct tagcagactg
     1501 ctccgtgaag ccttgcacca aacagctgca tctgaccttg gcccacaagt tctaccccca
     1561 ccaccagagg acgctggagc agctggccag agccatcccc ctgggccaca gctgccagtg
     1621 gaccgcagca ctctactccc gagacatgcg ctttgtgcac taccagaccc tgagagccct
     1681 attccagtac aaaccccaga acgtggatga gctgacgcta agtcctggtg actacatctt
     1741 tgtggacccc acgcagcagg acgaagccag cgagggctgg gtgattggga tctcacagcg
     1801 gacgggctgc cggggcttcc tgccggaaaa ctacacggat cgagccagtg agtctgacac
     1861 gtgggtgaag cacaggatgt acaccttcag tctagccaca gacctgaact ccagaaagga
     1921 tggtgaagcc agcagcagat gcagcgggga atttcttcca caaacggcaa ggagtcttag
     1981 cagcttacag gccttgcagg ctaccgttgc aaggaagagt gtgctggtgg ttcgccacgg
     2041 ggagagagtg gatcagatct tcgggaaggc atggctgcag caatgctcca ctcctgatgg
     2101 gaaatactac aggccagacc tgaatttccc ctgcagtctg cccagacgga gtcgtgggat
     2161 caaagacttt gaaaacgatc ccccattatc atcgtgtggc attttccagt ccagaattgc
     2221 aggggacgcg ctactggaca gtggtatcag aatcagctct gtgtttgcct ccccagccct
     2281 ccgctgtgtg cagacggcca aactcatcct ggaagaactc aaactggaga aaaaaatcaa
     2341 gatacgagtg gaacctggaa tctttgaatg gacaaaatgg gaagctggca agaccacccc
     2401 aaccctcatg agcctggaag agctgaaaga ggcaaatttc aacattgaca ctgattacag
     2461 gcccgcgttt cccctgtccg ccctcatgcc ggccgagagc taccaggagt acatggacag
     2521 gtgcacggcg agcatggtgc aaatcgtcaa cacctgtccg caggacacgg gtgtcatcct
     2581 aattgtgagt cacggctcca ctctggactc ctgcacgcgg ccactgctcg ggctgccgcc
     2641 ccgggaatgt ggggattttg cccaactcgt gagaaagatc ccttccctgg gcatgtgctt
     2701 ctgtgaagaa aataaagagg aaggaaaatg ggagttggtg aacccaccgg tgaagaccct
     2761 gacccacggg gcgaacgcag catttaactg gaggaactgg atctcaggca actgaaaccc
     2821 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     2881 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     2941 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     3001 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     3061 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     3121 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     3181 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     3241 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     3301 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     3361 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     3421 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     3481 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     3541 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     3601 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     3661 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     3721 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     3781 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     3841 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     3901 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     3961 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     4021 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4081 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4141 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4201 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4261 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4321 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4381 gcccagcttg gagcgaacga cctacaccga actgagata
//
