LOCUS       sequence-378793-        4533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human wild-type long-form UBASH3A in Gateway pDONR221 (for 
            N-terminal tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4533)
  TITLE     Concannon Lab UBASH3A Plasmids 2022
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDONR221-WT-long-UBASH3A-Ntag
FEATURES             Location/Qualifiers
     source          1..4533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(62..148)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     197..219
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     211..228
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     212..228
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    244..343
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2333..2432)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2449..2468)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2450..2468)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2473..2489)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2473..2489)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2591..2610)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2602..3411
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2669..2688)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3558..4146
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4047..4066
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4300..4317
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4476..4503)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
       61 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
      121 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
      181 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
      241 ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag
      301 caatgctttt ttataatgcc aactttgtac aaaaaagcag gcttcatggc agcgggggag
      361 acgcagctct acgccaaggt ctccaacaag ctcaagagcc gcagcagccc ctcgctcctg
      421 gagcccctcc tggccatggg cttcccggtg cacaccgcgc tgaaagcgtt ggcagccacg
      481 gggaggaaga cggcggagga ggccttggcc tggctgcatg atcattgcaa tgacccttcc
      541 ctagacgacc ccatccccca ggagtatgcc cttttcctct gtccaacggg gcccctgctg
      601 gaaaaacttc aagagttctg gagagagagc aagcgccagt gtgcaaagaa cagagctcat
      661 gaggtcttcc cacacgtgac actctgtgac ttcttcacgt gtgaagacca gaaggtggaa
      721 tgcctgtacg aggcgctgaa gagagctgga gacaggctcc tgggctcctt ccccacggcc
      781 gtgcctctgg ctctccactc ctccatcagc tacctcggct tcttcgtcag tggcagcccc
      841 gcagacgtca tccgggaatt cgccatgacc ttcgccacgg aagcatctct cttagcaggc
      901 acttccgttt cccgcttctg gattttcagc caggtgcctg gacatggccc taacctgagg
      961 ctgagcaatt taactagagc ctccttcgtg agccactaca tccttcaaaa atactgctcc
     1021 gtgaagcctt gcaccaaaca gctgcatctg accttggccc acaagttcta cccccaccac
     1081 cagaggacgc tggagcagct ggccagagcc atccccctgg gccacagctg ccagtggacc
     1141 gcagcactct actcccgaga catgcgcttt gtgcactacc agaccctgag agccctattc
     1201 cagtacaaac cccagaacgt ggatgagctg acgctaagtc ctggtgacta catctttgtg
     1261 gaccccacgc agcaggacga agccagcgag ggctgggtga ttgggatctc acagcggacg
     1321 ggctgccggg gcttcctgcc ggaaaactac acggatcgag ccagtgagtc tgacacgtgg
     1381 gtgaagcaca ggatgtacac cttcagtcta gccacagacc tgaactccag aaaggatggt
     1441 gaagccagca gcagatgcag cggggaattt cttccacaaa cggcaaggag tcttagcagc
     1501 ttacaggcct tgcaggctac cgttgcaagg aagagtgtgc tggtggttcg ccacggggag
     1561 agagtggatc agatcttcgg gaaggcatgg ctgcagcaat gctccactcc tgatgggaaa
     1621 tactacaggc cagacctgaa tttcccctgc agtctgccca ggcggagtcg tgggatcaaa
     1681 gactttgaaa acgatccccc attatcatcg tgtggcattt tccagtccag aattgcaggg
     1741 gacgcgctac tggacagtgg tatcagaatc agctctgtgt ttgcctcccc agccctccgc
     1801 tgtgtgcaga cggccaaact catcctggaa gaactcaaac tggagaaaaa aatcaagata
     1861 cgagtggaac ctggaatctt tgaatggaca aaatgggaag ctggcaaaac caccccaacc
     1921 ctcatgagcc tggaagagct gaaagaggca aatttcaaca ttgacactga ttacaggccc
     1981 gcgtttcccc tgtccgccct catgccggcc gagagctacc aggagtacat ggacaggtgc
     2041 acggcgagca tggtgcaaat cgtcaacacc tgcccgcagg acacgggtgt catcctaatt
     2101 gtgagtcacg gctccactct ggactcctgc acgcggccac tgctcgggct gccgccccgg
     2161 gaatgtgggg attttgccca actcgtgaga aagatccctt ccctgggcat gtgcttctgt
     2221 gaagaaaata aagaggaagg aaaatgggag ttggtgaacc caccggtgaa gaccctgacc
     2281 cacggggcga acgcagcatt taactggagg aactggatct caggcaactg aaacccagct
     2341 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     2401 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     2461 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     2521 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     2581 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     2641 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2701 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2761 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2821 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2881 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     2941 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     3001 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     3061 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     3121 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     3181 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     3241 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     3301 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     3361 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     3421 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     3481 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     3541 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3601 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3661 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3721 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3781 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3841 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3901 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3961 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4021 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4081 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4141 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4201 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     4261 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     4321 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     4381 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     4441 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     4501 tctgcttagt ttgatgcctg gcagtttatg gcg
//
