LOCUS       sequence-378795-        4419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human K202R short-form UBASH3A in Gateway pDONR221 (for N-terminal 
            tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ge Y, Paisie TK, Chen S, Concannon P
  TITLE     UBASH3A Regulates the Synthesis and Dynamics of TCR-CD3 Complexes.
  JOURNAL   J Immunol. 2019 Dec 1;203(11):2827-2836. doi: 
            10.4049/jimmunol.1801338. Epub 2019 Oct 28.
  PUBMED    31659016
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1801338"; journalName: "J Immunol"; date: 
            "2019-12-1- 1"; volume: "203"; issue: "11"; pages: "2827-2836"
COMMENT     Sequence Label: pDONR221-K202R-short-UBASH3A-Ntag
FEATURES             Location/Qualifiers
     source          1..4419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(32..49)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(32..48)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(41..63)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      112..198
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      290..317
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(476..493)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(647..1235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(727..746)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1382..2191)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2105..2124
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2183..2202
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2304..2320
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2304..2320
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2325..2344
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2325..2343
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2361..2460
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(join(4336..4419,1..16))
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ataaaatcat tatttggggc ccgagcttaa gactggccgt cgttttacaa cgtcgtgact
       61 gggaaaacat ccatgctagc gttaacgcga gagtagggaa ctgccaggca tcaaataaaa
      121 cgaaaggctc agtcggaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      181 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca acggcccgga
      241 gggtggcggg caggacgccc gccataaact gccaggcatc aaactaagca gaaggccatc
      301 ctgacggatg gcctttttgc gtttctacaa actcttcctg gctagcggta cgcgtattaa
      361 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
      421 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      481 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      541 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      601 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      661 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      721 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      781 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      841 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      901 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      961 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1021 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1081 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1141 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1201 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1261 ggtctgacgc tcagtggaac gacgcgtaac tcacgttaag ggattttggt catgagcttg
     1321 cgccgtcccg tcaagtcagc gtaatgctct gccagtgtta caaccaatta accaattctg
     1381 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     1441 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     1501 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     1561 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     1621 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
     1681 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     1741 gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     1801 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     1861 attcttctaa tacctggaat gctgtttttc cggggatcgc agtggtgagt aaccatgcat
     1921 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     1981 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     2041 acaactctgg cgcatcgggc ttcccataca agcgatagat tgtcgcacct gattgcccga
     2101 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     2161 gcctcgacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     2221 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     2281 tttgagacac gggccagagc tgccaggaaa cagctatgac catgtaatac gactcactat
     2341 aggggatatc agctggatgg caaataatga ttttattttg actgatagtg acctgttcgt
     2401 tgcaacaaat tgataagcaa tgctttctta taatgccaac tttgtacaag aaagctgggt
     2461 ttcagttgcc tgagatccag ttcctccagt taaatgctgc gttcgccccg tgggtcaggg
     2521 tcttcaccgg tgggttcacc aactcccatt ttccttcctc tttattttct tcacagaagc
     2581 acatgcccag ggaagggatc tttctcacga gttgggcaaa atccccacat tcccggggcg
     2641 gcagcccgag cagtggccgc gtgcaggagt ccagagtgga gccgtgactc acaattagga
     2701 tgacacccgt gtcctgcgga caggtgttga cgatttgcac catgctcgcc gtgcacctgt
     2761 ccatgtactc ctggtagctc tcggccggca tgagggcgga caggggaaac gcgggcctgt
     2821 aatcagtgtc aatgttgaaa tttgcctctt tcagctcttc caggctcatg agggttgggg
     2881 tggtcttgcc agcttcccat tttgtccatt caaagattcc aggttccact cgtatcttga
     2941 tttttttctc cagtttgagt tcttccagga tgagtttggc cgtctgcaca cagcggaggg
     3001 ctggggaggc aaacacagag ctgattctga taccactgtc cagtagcgcg tcccctgcaa
     3061 ttctggactg gaaaatgcca cacgatgata atgggggatc gttttcaaag tctttgatcc
     3121 cacgactccg tctgggcaga ctgcagggga aattcaggtc tggcctgtag tatttcccat
     3181 caggagtgga gcattgctgc agccatgcct tcccgaagat ctgatccact ctctccccgt
     3241 ggcgaaccac cagcacactc ttccttgcaa cggtagcctg caaggcctgt aagctgctaa
     3301 gactccttgc cgtttgtgga agaaattccc cgctgcatct gctgctggct tcaccatcct
     3361 ttctggagtt caggtctgtg gctagactga aggtgtacat cctgtgcttc acccacgtgt
     3421 cagactcact ggctcgatcc gtgtagtttt ccggcaggaa gccccggcag cccgtccgct
     3481 gtgagatccc aatcacccag ccctcgctgg cttcgtcctg ctgcgtgggg tccacaaaga
     3541 tgtagtcacc aggacttagc gtcagctcat ccacgttctg gggtttgtac tggaataggg
     3601 ctctcagggt ctggtagtgc acaaagcgca tgtctcggga gtagagtgct gcggtccact
     3661 ggcagctgtg gcccaggggg atggctctgg ccagctgctc cagcgtcctc tggtggtggg
     3721 ggtagaacct gtgggccaag gtcagatgca gctgtttggt gcaaggcttc acggagcagt
     3781 ctgctaagag agatgcttcc gtggcgaagg tcatggcgaa ttcccggatg acgtctgcgg
     3841 ggctgccact gacgaagaag ccgaggtagc tgatggagga gtggagagcc agaggcacgg
     3901 ccgtggggaa ggagcccagg agcctgtctc cagctctctt cagcgcctcg tacaggcatt
     3961 ccaccttctg gtcttcacac gtgaagaagt cacagagtgt cacgtgtggg aagacctcat
     4021 gagctctgtt ctttgcacac tggcgcttgc tctctctcca gaactcttga agtttttcca
     4081 gcaggggccc cgttggacag aggaaaaggg catactcctg ggggatgggg tcgtctaggg
     4141 aagggtcatt gcaatgatca tgcagccagg ccaaggcctc ctccgccgtc ttcctccccg
     4201 tggctgccaa cgctttcagc gcggtgtgca ccgggaagcc catggccagg aggggctcca
     4261 ggagcgaggg gctgctgcgg ctcttgagct tgttggagac cttggcgtag agctgcgtct
     4321 cccccgctgc catgaagcct gcttttttgt acaaagttgg cattataaaa aagcattgct
     4381 catcaatttg ttgcaacgaa caggtcacta tcagtcaaa
//
