LOCUS       sequence-378797-        4419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human W279A short-form UBASH3A in Gateway pDONR221 (for N-terminal 
            tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ge Y, Paisie TK, Chen S, Concannon P
  TITLE     UBASH3A Regulates the Synthesis and Dynamics of TCR-CD3 Complexes.
  JOURNAL   J Immunol. 2019 Dec 1;203(11):2827-2836. doi: 
            10.4049/jimmunol.1801338. Epub 2019 Oct 28.
  PUBMED    31659016
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1801338"; journalName: "J Immunol"; date: 
            "2019-12-1- 1"; volume: "203"; issue: "11"; pages: "2827-2836"
COMMENT     Sequence Label: pDONR221-W279A-short-UBASH3A-Ntag
FEATURES             Location/Qualifiers
     source          1..4419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1958..2057)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2074..2093)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2075..2093)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2098..2114)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2098..2114)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2216..2235)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2227..3036
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2294..2313)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3183..3771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3672..3691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3925..3942
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4101..4128)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4220..4306)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4355..4377
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4369..4386
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4370..4386
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(4402..4419,1..82)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
       61 actttgtaca aaaaagcagg cttcatggca gcgggggaga cgcagctcta cgccaaggtc
      121 tccaacaagc tcaagagccg cagcagcccc tcgctcctgg agcccctcct ggccatgggc
      181 ttcccggtgc acaccgcgct gaaagcgttg gcagccacgg ggaggaagac ggcggaggag
      241 gccttggcct ggctgcatga tcattgcaat gacccttccc tagacgaccc catcccccag
      301 gagtatgccc ttttcctctg tccaacgggg cccctgctgg aaaaacttca agagttctgg
      361 agagagagca agcgccagtg tgcaaagaac agagctcatg aggtcttccc acacgtgaca
      421 ctctgtgact tcttcacgtg tgaagaccag aaggtggaat gcctgtacga ggcgctgaag
      481 agagctggag acaggctcct gggctccttc cccacggccg tgcctctggc tctccactcc
      541 tccatcagct acctcggctt cttcgtcagt ggcagccccg cagacgtcat ccgggaattc
      601 gccatgacct tcgccacgga agcatctctc ttagcagact gctccgtgaa gccttgcacc
      661 aaacagctgc atctgacctt ggcccacaag ttctaccccc accaccagag gacgctggag
      721 cagctggcca gagccatccc cctgggccac agctgccagt ggaccgcagc actctactcc
      781 cgagacatgc gctttgtgca ctaccagacc ctgagagccc tattccagta caaaccccag
      841 aacgtggatg agctgacgct aagtcctggt gactacatct ttgtggaccc cacgcagcag
      901 gacgaagcca gcgagggcgc ggtgattggg atctcacagc ggacgggctg ccggggcttc
      961 ctgccggaaa actacacgga tcgagccagt gagtctgaca cgtgggtgaa gcacaggatg
     1021 tacaccttca gtctagccac agacctgaac tccagaaagg atggtgaagc cagcagcaga
     1081 tgcagcgggg aatttcttcc acaaacggca aggagtctta gcagcttaca ggccttgcag
     1141 gctaccgttg caaggaagag tgtgctggtg gttcgccacg gggagagagt ggatcagatc
     1201 ttcgggaagg catggctgca gcaatgctcc actcctgatg ggaaatacta caggccagac
     1261 ctgaatttcc cctgcagtct gcccagacgg agtcgtggga tcaaagactt tgaaaacgat
     1321 cccccattat catcgtgtgg cattttccag tccagaattg caggggacgc gctactggac
     1381 agtggtatca gaatcagctc tgtgtttgcc tccccagccc tccgctgtgt gcagacggcc
     1441 aaactcatcc tggaagaact caaactggag aaaaaaatca agatacgagt ggaacctgga
     1501 atctttgaat ggacaaaatg ggaagctggc aagaccaccc caaccctcat gagcctggaa
     1561 gagctgaaag aggcaaattt caacattgac actgattaca ggcccgcgtt tcccctgtcc
     1621 gccctcatgc cggccgagag ctaccaggag tacatggaca ggtgcacggc gagcatggtg
     1681 caaatcgtca acacctgtcc gcaggacacg ggtgtcatcc taattgtgag tcacggctcc
     1741 actctggact cctgcacgcg gccactgctc gggctgccgc cccgggaatg tggggatttt
     1801 gcccaactcg tgagaaagat cccttccctg ggcatgtgct tctgtgaaga aaataaagag
     1861 gaaggaaaat gggagttggt gaacccaccg gtgaagaccc tgacccacgg ggcgaacgca
     1921 gcatttaact ggaggaactg gatctcaggc aactgaaacc cagctttctt gtacaaagtt
     1981 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     2041 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     2101 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     2161 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     2221 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     2281 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     2341 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     2401 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     2461 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     2521 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2581 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2641 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2701 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2761 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2821 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2881 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2941 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     3001 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     3061 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     3121 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3181 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3241 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3301 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3361 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3421 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3481 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3541 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3601 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3661 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3721 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3781 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3841 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3901 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3961 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     4021 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     4081 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     4141 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     4201 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     4261 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     4321 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     4381 gccagtctta agctcgggcc ccaaataatg attttattt
//
