LOCUS       sequence-378810-        3551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTR plasmid Triticum aestivum sro1, amino acids 1-434, cultivar 
            SR3, no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Vogt S, Feijs K, Hosch S, De Masi R, Lintermann R, Loll B, 
            Wirthmueller L
  TITLE     The superior salinity tolerance of bread wheat cultivar Shanrong No.
            3 is unlikely to be caused by elevated Ta-sro1 poly-(ADP-ribose) 
            polymerase activity.
  JOURNAL   Plant Cell. 2022 Aug 18. pii: 6671231. doi: 10.1093/plcell/koac261.
  PUBMED    35980152
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2022 Aug 18. pii: 6671231. doi: 10.1093/plcell/koac261."
COMMENT     Sequence Label: pENTR4 Ta-sro1 WWE-PARP
FEATURES             Location/Qualifiers
     source          1..3551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..51
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      296..382
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      474..501
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     509..532
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    551..650
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1973..2072)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2184..2203)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2195..3004
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2262..2281)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(3094..3551,1..131)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
       61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      181 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcta
      241 gcatggatct cggggacgtc taactactaa gcgagagtag ggaactgcca ggcatcaaat
      301 aaaacgaaag gctcagtcgg aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      361 cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgtga agcaacggcc
      421 cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaacta agcagaaggc
      481 catcctgacg gatggccttt ttgcgtttct acaaactctt cctgttagtt agttacttaa
      541 gctcgggccc caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
      601 tgataagcaa tgctttttta taatgccaac tttgtacaaa aaagcaggct ccacatggaa
      661 aggaagactg gaatggtact ggatgaacat gtcctgaaag ctactgatcg caagaggaaa
      721 catgagtcgt ctgtgaagag tactggcaca gatgattttg ctgaggtctc tcagcatgct
      781 gatggttctg ctgtccttaa gaagtctaaa atgacctcct ctgctggtga tattctggag
      841 tgctataaga acttcaagac aagcggttcg cccgtgcgcg tgctttgtta ccaccagggt
      901 aaatggaggg actttccaga gcatgttgtg aatctggtac agcaggactt ccagttgaag
      961 aggccaatta ctaatgctgt attgaagaac cagcaagtcc tgttggactt tatgcacatg
     1021 gtctgcattg attcttcgat gaccacaaac aagccaatag catggattga tgttcatggt
     1081 aaccgcttct ttccagagtt atgtgctgga ctggtaacat ctaaaccatc tcagcatggg
     1141 aaaagtgacc ccagtggtaa atctaaagct ggtgagtgtg ctgggacttt gattttgaca
     1201 gctgaagctg aaagctcgag ttcagattct gttgatgcag tactccctca tgctaagaag
     1261 gttaataaca tactggagga aggacaggag gtacataatt atgctgctgt tgaaaataaa
     1321 gctggtcctg caatctgttt gaatgaacct gctagtggaa acatacatgc tgctacttat
     1381 aagcagaaaa ttggccaacc tgttgattca gctgtgcgca agttattgct tgaaggggca
     1441 ggccaacctt ttagtgaaga aaatattatt ggcatctaca gaactccact ggtagatcag
     1501 caagggcggg ctcgcttcaa tcttttccag aaggaacttg aggccaccaa aatgcatcgt
     1561 ggcaacgcaa atgtgcgcta cgcgtggcta ccttgttcca aggatactat ggaggagatg
     1621 atgatgcgtg gtgttctgga agttacaaaa cccatgctgg ggccagtgta tggcattggt
     1681 acacatcttg ctccagcgaa ctgtgcccaa acctgcgcta gctactcaga tattgacgaa
     1741 aatggcatca tgaggatgat gctgtgccgt gttataatgg gtaatgttga ggttgtactt
     1801 cctggatcaa agcaattcca gccaaccaat gaaagatttg atagtggtgt ggatgatctt
     1861 caaaagccaa agcactatat catatgggat gctaatgtgc atcgacacat ctatgctgaa
     1921 tatgctgttg ttatcaaagc accttccatg accaataact cgagatatct agacccagct
     1981 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     2041 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgcagctct ggcccgtgtc
     2101 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
     2161 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     2221 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     2281 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
     2341 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     2401 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     2461 tggtgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
     2521 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     2581 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     2641 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     2701 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     2761 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     2821 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     2881 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     2941 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     3001 ctaatcagaa ttggttaatt ggttgtaaca ttattcagat tgggccccgt tccactgagc
     3061 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3121 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3181 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3241 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3301 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3361 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3421 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3481 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3541 cggcagggtc g
//
