LOCUS       sequence-378812-        4146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no 
            stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Vogt S, Feijs K, Hosch S, De Masi R, Lintermann R, Loll B, 
            Wirthmueller L
  TITLE     The superior salinity tolerance of bread wheat cultivar Shanrong No.
            3 is unlikely to be caused by elevated Ta-sro1 poly-(ADP-ribose) 
            polymerase activity.
  JOURNAL   Plant Cell. 2022 Aug 18. pii: 6671231. doi: 10.1093/plcell/koac261.
  PUBMED    35980152
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2022 Aug 18. pii: 6671231. doi: 10.1093/plcell/koac261."
COMMENT     Sequence Label: pENTR4 PARP2 E614Q
FEATURES             Location/Qualifiers
     source          1..4146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      111..197
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      289..316
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     324..347
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    366..465
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2383..2482)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2594..2613)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2605..3414
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2672..2691)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3504..4092
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3993..4012
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgctagcatg
       61 gatctcgggg acgtctaact actaagcgag agtagggaac tgccaggcat caaataaaac
      121 gaaaggctca gtcggaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      181 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgtgaagcaa cggcccggag
      241 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aactaagcag aaggccatcc
      301 tgacggatgg cctttttgcg tttctacaaa ctcttcctgt tagttagtta cttaagctcg
      361 ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata
      421 agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggctccacc atggcgaaca
      481 agctcaaagt cgacgaactc cgtttaaaac tcgccgagcg tggactcagt actactggag
      541 tcaaagccgt tctggtggag aggcttgaag aggctatcgc agaagacact aagaaggaag
      601 aatcaaagag caagaggaaa agaaattctt ctaatgatac ttatgaatcg aacaaattga
      661 ttgcaattgg cgaatttcgt gggatgattg tgaaggaatt gcgtgaggaa gctattaaga
      721 gaggcttaga tacaacagga accaaaaagg atcttcttga gaggctttgc aatgatgcta
      781 ataacgtttc caatgcacca gtcaaatcca gtaatgggac agatgaagct gaagatgaca
      841 acaatggctt tgaagaagaa aagaaagaag agaaaatcgt aaccgcgaca aagaagggtg
      901 cagcggtgtt agatcagtgg attcctgatg agataaagag tcagtaccat gttctacaaa
      961 ggggtgatga tgtttatgat gctatcttaa atcagacaaa tgtcagggat aataataaca
     1021 agttctttgt cctacaagtc ctagagtcgg atagtaaaaa gacatacatg gtttacacca
     1081 gatggggaag agttggtgtg aaaggacaaa gtaagctaga tgggccttat gactcatggg
     1141 atcgtgcgat agagatattt accaataagt tcaatgacaa gacaaagaat tattggtctg
     1201 acagaaagga gtttatccca catcccaagt cctatacatg gctcgaaatg gattacggaa
     1261 aagaggaaaa tgattcaccg gtcaataatg atattccgag ttcatcttcc gaagttaaac
     1321 ctgaacaatc aaaactagat actcgggttg ccaagttcat ctctcttata tgtaatgtca
     1381 gcatgatggc acagcatatg atggaaatag gatataacgc taacaaattg ccactcggca
     1441 agataagcaa gtccacaatt tcaaagggtt atgaagtgct gaagagaata tcggaggtga
     1501 ttgatcggta tgatagaacg aggcttgagg aactgagtgg agagttctac acagtgatac
     1561 ctcatgattt tggttttaag aaaatgagcc agtttgttat agacactcct caaaagttga
     1621 aacagaaaat tgaaatggtt gaagcattag gtgagattga actcgcaaca aagttgttgt
     1681 ccgtcgaccc gggattgcag gatgatcctt tatattatca ctaccagcaa cttaattgtg
     1741 gtttgacgcc agtaggaaat gattcagagg agttctctat ggttgctaat tacatggaga
     1801 acactcatgc aaagacgcat tcgggatata cggttgagat tgctcaacta tttagagctt
     1861 cgagagctgt tgaagctgat cgattccaac agttttcaag ttcgaagaac aggatgctac
     1921 tctggcacgg ttcacgtctc actaactggg ctggtatttt atctcaaggt ctgcgaatag
     1981 ctcctcctga agcgcctgta actggttaca tgtttggaaa aggggtttac tttgcggata
     2041 tgttctccaa gagtgcgaac tattgctatg ccaacactgg cgctaatgat ggcgttctgc
     2101 tcctctgcga ggttgctttg ggagacatga atgaacttct gtattcagat tataacgcgg
     2161 ataatctacc cccgggaaag ctaagcacaa aaggtgtggg gaaaacagca ccaaacccat
     2221 cagaggctca aacactagaa gacggtgttg ttgttccact tggcaaacca gtggaacgtt
     2281 catgctccaa ggggatgttg ttgtacaacc aatatatagt ctacaatgtg gaacaaatca
     2341 agatgcgtta tgtgatccaa gtcaaattca actacaagca cgacccagct ttcttgtaca
     2401 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     2461 gtcaaaataa aatcattatt tgccatccag ctgcagctct ggcccgtgtc tcaaaatctc
     2521 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     2581 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     2641 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     2701 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     2761 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     2821 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tggtgatgca
     2881 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     2941 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     3001 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     3061 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     3121 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     3181 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     3241 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     3301 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     3361 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     3421 ttggttaatt ggttgtaaca ttattcagat tgggccccgt tccactgagc gtcagacccc
     3481 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3541 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3601 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3661 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3721 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3781 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3841 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3901 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3961 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4021 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4081 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4141 tttgct
//
