LOCUS       sequence-378813-        3983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no 
            stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Vogt S, Feijs K, Hosch S, De Masi R, Lintermann R, Loll B, 
            Wirthmueller L
  TITLE     The superior salinity tolerance of bread wheat cultivar Shanrong No.
            3 is unlikely to be caused by elevated Ta-sro1 poly-(ADP-ribose) 
            polymerase activity.
  JOURNAL   Plant Cell. 2022 Aug 18. pii: 6671231. doi: 10.1093/plcell/koac261.
  PUBMED    35980152
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2022 Aug 18. pii: 6671231. doi: 10.1093/plcell/koac261."
COMMENT     Sequence Label: pENTR4 Ta-sro1 SR3
FEATURES             Location/Qualifiers
     source          1..3983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      40..628
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     529..548
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      793..879
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      971..998
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     1006..1029
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    1048..1147
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2902..3001)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3113..3132)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3124..3933
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3191..3210)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
       61 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
      121 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
      181 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
      241 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
      301 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
      361 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
      421 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
      481 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
      541 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
      601 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
      661 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
      721 taaccgtatt accgctagca tggatctcgg ggacgtctaa ctactaagcg agagtaggga
      781 actgccaggc atcaaataaa acgaaaggct cagtcggaag actgggcctt tcgttttatc
      841 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
      901 gttgtgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
      961 caaactaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttcct
     1021 gttagttagt tacttaagct cgggccccaa ataatgattt tattttgact gatagtgacc
     1081 tgttcgttgc aacaaattga taagcaatgc ttttttataa tgccaacttt gtacaaaaaa
     1141 gcaggctcca catggaaagg aagactggaa tggtactgga tgaacatgtc ctgaaagcta
     1201 ctgatcgcaa gaggaaacat gagtcgtctg tgaagagtac tggcacagat gattttgctg
     1261 aggtctctca gcatgctgat ggttctgctg tccttaagaa gtctaaaatg acctcctctg
     1321 ctggtgatat tctggagtgc tataagaact tcaagacaag cggttcgccc gtgcgcgtgc
     1381 tttgttacca ccagggtaaa tggagggact ttccagagca tgttgtgaat ctggtacagc
     1441 aggacttcca gttgaagagg ccaattacta atgctgtatt gaagaaccag caagtcctgt
     1501 tggactttat gcacatggtc tgcattgatt cttcgatgac cacaaacaag ccaatagcat
     1561 ggattgatgt tcatggtaac cgcttctttc cagagttatg tgctggactg gtaacatcta
     1621 aaccatctca gcatgggaaa agtgacccca gtggtaaatc taaagctggt gagtgtgctg
     1681 ggactttgat tttgacagct gaagctgaaa gctcgagttc agattctgtt gatgcagtac
     1741 tccctcatgc taagaaggtt aataacatac tggaggaagg acaggaggta cataattatg
     1801 ctgctgttga aaataaagct ggtcctgcaa tctgtttgaa tgaacctgct agtggaaaca
     1861 tacatgctgc tacttataag cagaaaattg gccaacctgt tgattcagct gtgcgcaagt
     1921 tattgcttga aggggcaggc caacctttta gtgaagaaaa tattattggc atctacagaa
     1981 ctccactggt agatcagcaa gggcgggctc gcttcaatct tttccagaag gaacttgagg
     2041 ccaccaaaat gcatcgtggc aacgcaaatg tgcgctacgc gtggctacct tgttccaagg
     2101 atactatgga ggagatgatg atgcgtggtg ttctggaagt tacaaaaccc atgctggggc
     2161 cagtgtatgg cattggtaca catcttgctc cagcgaactg tgcccaaacc tgcgctagct
     2221 actcagatat tgacgaaaat ggcatcatga ggatgatgct gtgccgtgtt ataatgggta
     2281 atgttgaggt tgtacttcct ggatcaaagc aattccagcc aaccaatgaa agatttgata
     2341 gtggtgtgga tgatcttcaa aagccaaagc actatatcat atgggatgct aatgtgcatc
     2401 gacacatcta tgctgaatat gctgttgtta tcaaagcacc ttccatgacc aatgaatact
     2461 tggctagaga agatactgca tccaacatat ctgagataag aaattctggt tcagcagaaa
     2521 gtgtaatcaa ggacgatagt tccgaaacca tggcatctcc agctgataaa caacaagcac
     2581 ccaggtttgg gcgtgcccca actcgaaggc ctcctacttc accctggatg cccctcccaa
     2641 tgcttttcgc tgccatctcc acaaaagttc ctcgttctga catggatgta atccatggac
     2701 actatgaaga attcaagagg agaaagataa gcaggcccga gtttgtgaga cggctaagac
     2761 agatcttcgg cgacaagctg ctggtttcta cagtaatgag gctgcaacct aaggtagtgg
     2821 cacccatggc aggtgctgaa gtgctaccaa gaggagcacc cggcacggga gggagcagca
     2881 cctccaactc gagatatcta gacccagctt tcttgtacaa agttggcatt ataagaaagc
     2941 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     3001 gccatccagc tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     3061 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     3121 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     3181 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     3241 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     3301 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     3361 ccgaccatca agcattttat ccgtactcct ggtgatgcat ggttactcac cactgcgatc
     3421 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     3481 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     3541 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     3601 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     3661 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     3721 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     3781 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     3841 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     3901 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacat
     3961 tattcagatt gggccccgtt cca
//
