LOCUS       sequence-37882-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1216
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accatggtgg cggaagttcc tatacctttt gaagaatagg aacttcggaa taggaacttc
       61 ggcgcgccgg gcacactcag ggtcgcctga gccgctgcct gatccctggc caccgtccgt
      121 ctgaggtgtc cggcgctccc agccaccttt caagccctcg ggtgtgacca atccgcggcc
      181 aagccgagac ccttgcagcc aatcgctagg cggctgggat ttcaggggcg ggcgcgactt
      241 gccctcagct ccccacctcc ccgtcccggg gcgacagtga cataagcaaa tctgggcaga
      301 gaggtggaag tcaggtccct gtggacgtgg gaggcggtcc cttcctcttt tggctccaaa
      361 gtgcgaaagg aggcagacac agcggtctcc attacagcca gggcaagggg gctaatagct
      421 ccctagaact gatggaagtc tccattctgg gaagcatttc ttattcctat cgaggtttta
      481 aaaacacctg gcgatggtcc tctggaaatt gagggtttaa aatttcagca agagctgaag
      541 ttaaacttcc agaaacattt actatatata ggtttagtgc ttaagaaaca aagatggaat
      601 gcaaagtagc attatagacc aggagagggc agagacaggt ggctggagtt agagaagcaa
      661 attcactccc tctcttcccc tcaattctgg agaacttgct gttgaggcaa ggtacctggg
      721 cctgaccagg tgaagggagt gatatatgct ggcagggcgt ggccccagta gagtgcagaa
      781 gagctgcctc tgtcatgaag caggtctgct gagctgggcg aggctgcgct tcctccgcca
      841 ggccattccc tggcagctgc aagctctctt cttggctctg gtgcaaactt cctgcttta
//
