LOCUS       sequence-378849-        9769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter vector to visualize all of cell cycle phases..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Ohta Y, Fujii M, Takahashi S, Takano A, Nanki K, Matano M, Hanyu H, 
            Saito M, Shimokawa M, Nishikori S, Hatano Y, Ishii R, Sawada K, 
            Machinaga A, Ikeda W, Imamura T, Sato T
  TITLE     Cell-matrix interface regulates dormancy in human colon cancer stem 
            cells.
  JOURNAL   Nature. 2022 Jul 7. pii: 10.1038/s41586-022-05043-y. doi: 
            10.1038/s41586-022-05043-y.
  PUBMED    35798028
REFERENCE   2  (bases 1 to 9769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Jul 7. pii: 10.1038/s41586-022-05043-y. doi: 
            10.1038/s41586-022-05043-y."
COMMENT     Sequence Label: Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2
FEATURES             Location/Qualifiers
     source          1..9769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(36..140)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(166..621)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(303..324)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     515..534
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     748..770
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     762..779
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     763..779
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   909..971
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1475..1678
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1629..1649
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1675..1699
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3030..3086
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3111..3128
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3132..3164
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             3177..3197
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3204..3914
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             3918..4247
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     CDS             complement(4725..4736)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4845..4861)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4846..4862)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4857..4874)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4863..4974
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5134..5345
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5358..5626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5653..6252
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5653..5672)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6149..6169
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6374..6495
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6411..6430)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6465..6484
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7258..7292
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7783..7799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7783..7799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7796..7818)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7807..7823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7831..7837,7838..7855,7856..7861))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7876..7897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8014..8031)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8185..8773)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8265..8284)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8944..9735,9736..9769,1..35))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9567..9586
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
       61 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
      121 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
      181 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
      241 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
      301 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
      361 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
      421 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
      481 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
      541 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
      601 atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc
      661 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
      721 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
      781 agcgcgcctc gttcattcac gtttttgaac ccgtggagga cgggcagact cgcggtgcaa
      841 atgtgtttta cagcgtgatg gagcagatga agatgctcga cacgctgcag aacacgcagc
      901 tagattaacc ctagaaagat aatcatattg tgacgtacgt taaagataat catgtgtaaa
      961 attgacgcat gtgttttatc ggtctgtata tcgaggttta tttattaatt tgaatagata
     1021 ttaagtttta ttatatttac acttacatac taataataaa ttcaacaaac aatttattta
     1081 tgtttattta tttattaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac
     1141 ttttatgagg gacagccccc ccccaaagcc cccagggatg taattacgtc cctcccccgc
     1201 tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc cccgcatccc
     1261 cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc
     1321 tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac ggggaaaagg
     1381 cctccacggc cactagtatt atgcccagta catgacctta tgggactttc ctacttggca
     1441 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     1501 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     1561 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     1621 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtttatataa gcagagctcg
     1681 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     1741 attctagaac catggccgaa gggagcgtgg caagacagcc cgatctgctg acatgcgacg
     1801 acgaaccaat ccacattcca ggagctatcc agcctcatgg actgctgctg gctctggccg
     1861 ctgacatgac tatcgtggca ggctctgata acctgccaga gctgaccggg ctggctatcg
     1921 gagcactgat tggccggtcc gcagccgacg tgttcgattc tgagactcac aatagactga
     1981 ccatcgccct ggctgaacct ggggctgcag tgggagcccc aattaccgtc ggcttcacaa
     2041 tgcgaaagga cgctggcttt atcgggtcct ggcaccgcca tgatcagctg atttttctgg
     2101 agctggaacc acctcagaga gacgtggcag agccacaggc tttctttcgg agaacaaaca
     2161 gcgccatcag gcgcctgcag gccgctgaga ctctggaatc cgcttgcgca gccgctgcac
     2221 aggaagtgag gaagatcacc gggttcgatc gcgtcatgat ctacagattc gcctcagact
     2281 ttagcggaga agtgatcgca gaagatcgct gtgccgaggt cgaaagtaaa ctgggcctgc
     2341 attacccagc ctcaaccgtg cctgcacagg cacgacggct gtatacaatc aaccccgtgc
     2401 ggatcattcc cgacattaat tacagaccag tgcccgtcac acctgacctg aacccagtga
     2461 ctgggaggcc catcgatctg agcttcgcca ttctgcgcag cgtgtcccct gtccacctgg
     2521 agtttatgag gaatatcgga atgcatggca ccatgtctat cagtattctg agaggcgaaa
     2581 ggctgtgggg gctgatcgtg tgccaccatc ggacacctta ctatgtggac ctggacggca
     2641 ggcaggcttg tgaactggtg gctcaggtgc tggcttggca gattggcgtg atggaagaac
     2701 tcgagtctag agctagcgaa ttccctagga tggagcaacg ccgggttacc gacttctttg
     2761 ccaggcgcag accaggtcca cccaggattg cccctcccaa actcgcctgt cggacccctt
     2821 ctccagccag acccgcactg agagctccag cctctgccac ttccggcagc aggaaacggg
     2881 ctagacctcc agctgcacca ggacgggatc aggcccggcc tcctgctcgc gcagccgctc
     2941 gcctgtccgt ggatgaggtc agctcaccca gcacacccga agccccagac atccccgcat
     3001 gccctagtcc cgggcagaag atcaagaagg ccaccaactt cagcctgctg aagcaggccg
     3061 gcgacgtgga ggagaacccc ggccccccta ggaccgccat gcggggttct catcatcatc
     3121 atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgaga
     3181 acctgtactt ccagggctcg agcatggtgt ctaagggcga agagctgatt aaggagaaca
     3241 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
     3301 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     3361 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
     3421 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
     3481 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     3541 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
     3601 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc
     3661 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
     3721 atctgatcgc aaacgccaag accacatata gatccaagaa acccgctaag aacctcaaga
     3781 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga
     3841 cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg
     3901 ggcacaagct taatcatatg aatcccagta tgaagcagaa acaagaagaa atcaaagaga
     3961 atataaagaa tagttctgtc ccaagaagaa ctctgaagat gattcagcct tctgcatctg
     4021 gatctcttgt tggaagagaa aatgagctgt ccgcaggctt gtccaaaagg aaacatcgga
     4081 atgaccactt aacatctaca acttccagcc ctggggttat tgtcccagaa tctagtgaaa
     4141 ataaaaatct tggaggagtc acccaggagt catttgatct tatgattaaa gaaaatccat
     4201 cctctcagta ttggaaggaa gtggcagaaa aacggagaaa ggcgctgtaa atatgtcaac
     4261 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     4321 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     4381 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     4441 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     4501 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     4561 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4621 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     4681 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4741 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4801 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     4861 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     4921 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     4981 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5041 ttgggaagac aatagcaggc atgggtaccg aattcgaatt taaatcggat ccgcggccgc
     5101 gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     5161 ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg
     5221 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     5281 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     5341 cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag
     5401 gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa
     5461 ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc
     5521 tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc
     5581 aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg
     5641 cgcctacgct agatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5701 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     5761 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     5821 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     5881 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     5941 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6001 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6061 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6121 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6181 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6241 cccggtgcct gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6301 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6361 catgcgttaa ctaaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6421 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6481 catcaatgta tcttatcatg tctggaattg actcaaatga tgtcaattag tctatcagaa
     6541 gctcatctgg tctcccttcc gggggacaag acatccctgt ttaatattta aacagcagtg
     6601 ttcccaaact gggttcttat atcccttgct ctggtcaacc aggttgcagg gtttcctgtc
     6661 ctcacaggaa cgaagtccct aaagaaacag tggcagccag gtttagcccc ggaattgact
     6721 ggattccttt tttagggccc attggtatgg ctttttcccc gtatcccccc aggtgtctgc
     6781 aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg
     6841 cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg
     6901 gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg
     6961 ggctttgggg gggggctgtc cctgatatct ataacaagaa aatatatata taataagtta
     7021 tcacgtaagt agaacatgaa ataacaatat aattatcgta tgagttaaat cttaaaagtc
     7081 acgtaaaaga taatcatgcg tcattttgac tcacgcggtc gttatagttc aaaatcagtg
     7141 acacttaccg cattgacaag cacgcctcac gggagctcca agcggcgact gagatgtcct
     7201 aaatgcacag cgacggattc gcgctattta gaaagagaga gcaatatttc aagaatgcat
     7261 gcgtcaattt tacgcagact atctttctag ggttaatcta gctgcatcag gatcatatcg
     7321 tcgggtcttt tttccggctc agtcatcgcc caagctggcg ctatctgggc atcggggagg
     7381 aagaagcccg tgccttttcc cgcgaggttg aagcggcatg gaaagagttt gccgaggatg
     7441 actgctgctg cattgacgtt gagcgaaaac gcacgtttac catgatgatt cgggaaggtg
     7501 tggccatgca cgcctttaac ggtgaactgt tcgttcaggc cacctgggat accagttcgt
     7561 cgcggctttt ccggacacag ttccggatgg tcagcccgaa gcgcatcagc aacccgaaca
     7621 ataccggcga cagccggaac tgccgtgccg gtgtgcagat taatgacagc ggtgcggcgc
     7681 tgggatatta cgtcagcgag gacgggtatc ctggctggat gccgcagaaa tggacatgga
     7741 taccccgtga gttacccggc gggcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     7801 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7861 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7921 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7981 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8041 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8101 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8161 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8221 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8281 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8341 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8401 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8461 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8521 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8581 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8641 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8701 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8761 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8821 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8881 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8941 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9001 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9061 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9121 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9181 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9241 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9301 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9361 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9421 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9481 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9541 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9601 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9661 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9721 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaag
//
