LOCUS       sequence-378850-        9901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter vector for visualization of G0 and other cell 
            cycle phases..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Ohta Y, Fujii M, Takahashi S, Takano A, Nanki K, Matano M, Hanyu H, 
            Saito M, Shimokawa M, Nishikori S, Hatano Y, Ishii R, Sawada K, 
            Machinaga A, Ikeda W, Imamura T, Sato T
  TITLE     Cell-matrix interface regulates dormancy in human colon cancer stem 
            cells.
  JOURNAL   Nature. 2022 Jul 7. pii: 10.1038/s41586-022-05043-y. doi: 
            10.1038/s41586-022-05043-y.
  PUBMED    35798028
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Jul 7. pii: 10.1038/s41586-022-05043-y. doi: 
            10.1038/s41586-022-05043-y."
COMMENT     Sequence Label: Fucci(CA) hCdt1-iRFP hGeminin-iRFP
FEATURES             Location/Qualifiers
     source          1..9901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1440..1643
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1594..1614
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1640..1664
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2995..3051
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4015..4344
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     CDS             complement(4822..4833)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4942..4958)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4943..4959)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4954..4971)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4960..5071
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5231..5442
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5455..5723
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5750..6349
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5750..5769)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6246..6266
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6471..6592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6508..6527)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6562..6581
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7355..7389
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7880..7896)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7880..7896)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7893..7915)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7904..7920
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7928..7934,7935..7952,7953..7958))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7973..7994
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8111..8128)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8282..8870)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8362..8381)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9041..9832,9833..9901))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9664..9683
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtattatgcc cagtacatga
     1381 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     1441 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     1501 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     1561 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     1621 gggaggttta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     1681 cacgctgttt tgacctccat agaagattct agaaccatgg ccgaagggag cgtggcaaga
     1741 cagcccgatc tgctgacatg cgacgacgaa ccaatccaca ttccaggagc tatccagcct
     1801 catggactgc tgctggctct ggccgctgac atgactatcg tggcaggctc tgataacctg
     1861 ccagagctga ccgggctggc tatcggagca ctgattggcc ggtccgcagc cgacgtgttc
     1921 gattctgaga ctcacaatag actgaccatc gccctggctg aacctggggc tgcagtggga
     1981 gccccaatta ccgtcggctt cacaatgcga aaggacgctg gctttatcgg gtcctggcac
     2041 cgccatgatc agctgatttt tctggagctg gaaccacctc agagagacgt ggcagagcca
     2101 caggctttct ttcggagaac aaacagcgcc atcaggcgcc tgcaggccgc tgagactctg
     2161 gaatccgctt gcgcagccgc tgcacaggaa gtgaggaaga tcaccgggtt cgatcgcgtc
     2221 atgatctaca gattcgcctc agactttagc ggagaagtga tcgcagaaga tcgctgtgcc
     2281 gaggtcgaaa gtaaactggg cctgcattac ccagcctcaa ccgtgcctgc acaggcacga
     2341 cggctgtata caatcaaccc cgtgcggatc attcccgaca ttaattacag accagtgccc
     2401 gtcacacctg acctgaaccc agtgactggg aggcccatcg atctgagctt cgccattctg
     2461 cgcagcgtgt cccctgtcca cctggagttt atgaggaata tcggaatgca tggcaccatg
     2521 tctatcagta ttctgagagg cgaaaggctg tgggggctga tcgtgtgcca ccatcggaca
     2581 ccttactatg tggacctgga cggcaggcag gcttgtgaac tggtggctca ggtgctggct
     2641 tggcagattg gcgtgatgga agaactcgag tctagagcta gcgaattccc taggatggag
     2701 caacgccggg ttaccgactt ctttgccagg cgcagaccag gtccacccag gattgcccct
     2761 cccaaactcg cctgtcggac cccttctcca gccagacccg cactgagagc tccagcctct
     2821 gccacttccg gcagcaggaa acgggctaga cctccagctg caccaggacg ggatcaggcc
     2881 cggcctcctg ctcgcgcagc cgctcgcctg tccgtggatg aggtcagctc acccagcaca
     2941 cccgaagccc cagacatccc cgcatgccct agtcccgggc agaagatcaa gaaggccacc
     3001 aacttcagcc tgctgaagca ggccggcgac gtggaggaga accccggccc ccctaggatg
     3061 gccgaaggga gcgtggcaag acagcccgat ctgctgacat gcgacgacga accaatccac
     3121 attccaggag ctatccagcc tcatggactg ctgctggctc tggccgctga catgactatc
     3181 gtggcaggct ctgataacct gccagagctg accgggctgg ctatcggagc actgattggc
     3241 cggtccgcag ccgacgtgtt cgattctgag actcacaata gactgaccat cgccctggct
     3301 gaacctgggg ctgcagtggg agccccaatt accgtcggct tcacaatgcg aaaggacgct
     3361 ggctttatcg ggtcctggca ccgccatgat cagctgattt ttctggagct ggaaccacct
     3421 cagagagacg tggcagagcc acaggctttc tttcggagaa caaacagcgc catcaggcgc
     3481 ctgcaggccg ctgagactct ggaatccgct tgcgcagccg ctgcacagga agtgaggaag
     3541 atcaccgggt tcgatcgcgt catgatctac agattcgcct cagactttag cggagaagtg
     3601 atcgcagaag atcgctgtgc cgaggtcgaa agtaaactgg gcctgcatta cccagcctca
     3661 accgtgcctg cacaggcacg acggctgtat acaatcaacc ccgtgcggat cattcccgac
     3721 attaattaca gaccagtgcc cgtcacacct gacctgaacc cagtgactgg gaggcccatc
     3781 gatctgagct tcgccattct gcgcagcgtg tcccctgtcc acctggagtt tatgaggaat
     3841 atcggaatgc atggcaccat gtctatcagt attctgagag gcgaaaggct gtgggggctg
     3901 atcgtgtgcc accatcggac accttactat gtggacctgg acggcaggca ggcttgtgaa
     3961 ctggtggctc aggtgctggc ttggcagatt ggcgtgatgg aagaactcga gcatatgaat
     4021 cccagtatga agcagaaaca agaagaaatc aaagagaata taaagaatag ttctgtccca
     4081 agaagaactc tgaagatgat tcagccttct gcatctggat ctcttgttgg aagagaaaat
     4141 gagctgtccg caggcttgtc caaaaggaaa catcggaatg accacttaac atctacaact
     4201 tccagccctg gggttattgt cccagaatct agtgaaaata aaaatcttgg aggagtcacc
     4261 caggagtcat ttgatcttat gattaaagaa aatccatcct ctcagtattg gaaggaagtg
     4321 gcagaaaaac ggagaaaggc gctgtaaata tgtcaacctc tggattacaa aatttgtgaa
     4381 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4441 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4501 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4561 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4621 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4681 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4741 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4801 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4861 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4921 ctttgggccg cctccccgca tcgataccgt cgacctcgac tgtgccttct agttgccagc
     4981 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5041 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     5101 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     5161 ggtaccgaat tcgaatttaa atcggatccg cggccgcgaa ggatctgcga tcgctccggt
     5221 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     5281 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     5341 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     5401 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     5461 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     5521 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     5581 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     5641 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     5701 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga tgaccgagta
     5761 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     5821 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     5881 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     5941 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     6001 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     6061 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6121 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6181 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     6241 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6301 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca
     6361 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6421 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gcgttaacta aacttgttta
     6481 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6541 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6601 ggaattgact caaatgatgt caattagtct atcagaagct catctggtct cccttccggg
     6661 ggacaagaca tccctgttta atatttaaac agcagtgttc ccaaactggg ttcttatatc
     6721 ccttgctctg gtcaaccagg ttgcagggtt tcctgtcctc acaggaacga agtccctaaa
     6781 gaaacagtgg cagccaggtt tagccccgga attgactgga ttcctttttt agggcccatt
     6841 ggtatggctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc
     6901 gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc
     6961 cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg
     7021 ccccctagcg ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct
     7081 gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata
     7141 acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca
     7201 ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac
     7261 gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg
     7321 ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc
     7381 tttctagggt taatctagct gcatcaggat catatcgtcg ggtctttttt ccggctcagt
     7441 catcgcccaa gctggcgcta tctgggcatc ggggaggaag aagcccgtgc cttttcccgc
     7501 gaggttgaag cggcatggaa agagtttgcc gaggatgact gctgctgcat tgacgttgag
     7561 cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt
     7621 gaactgttcg ttcaggccac ctgggatacc agttcgtcgc ggcttttccg gacacagttc
     7681 cggatggtca gcccgaagcg catcagcaac ccgaacaata ccggcgacag ccggaactgc
     7741 cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg gatattacgt cagcgaggac
     7801 gggtatcctg gctggatgcc gcagaaatgg acatggatac cccgtgagtt acccggcggg
     7861 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7921 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     7981 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8041 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8101 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8161 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8221 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8281 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8341 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8401 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8461 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8521 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8581 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8641 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8701 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8761 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8821 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8881 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8941 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9001 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9061 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9121 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9181 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9241 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9301 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9361 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9421 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9481 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9541 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9601 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9661 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9721 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9781 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9841 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9901 t
//
