LOCUS       sequence-378855-       10001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ti plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 
            detection in the nuclei of plant cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10001)
  AUTHORS   Exposito-Rodriguez M, Reeder B, Brooke GN, Hough MA, Laissue PP, 
            Mullineaux PM
  TITLE     A novel glutathione peroxidase-based biosensor disentangles 
            differential subcellular accumulation of H2O2 and lipid 
            hydroperoxides
  JOURNAL   bioRxiv 2024.01.18.576236
REFERENCE   2  (bases 1 to 10001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.18.576236"
COMMENT     Sequence Label: pICH-nuc-roGFP2-synORP1
FEATURES             Location/Qualifiers
     source          1..10001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    144..168
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(291..879)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(371..390)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1050..1844)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(1845..1949)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2232..2426
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2492..3565)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3994..4623)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4887..4911
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        5089..5268
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        5121..5304
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5325..6119
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5379..5398)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5989..6008
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6145..6397
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7413..7758
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     7701..7721
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             7949..7969
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      join(9375..10001,1..81)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg gtccagggcg aattttgcga
       61 caacatgtcg aggctcagca ggaccgctac tagaggatgc acatgtgacc gagggacacg
      121 aagtggttta aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag
      181 agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca
      241 tttgtatgtg ccaaccacag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      301 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      361 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      421 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      481 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      541 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      601 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      661 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      721 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      781 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      841 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      901 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      961 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1021 agtatatatg agtaaacttg gtctgacagc taaaacaatt catccagtaa aatataatat
     1081 tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt
     1141 tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc
     1201 gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg
     1261 ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt
     1321 aaaaaatcat acagctcgcg cggatcttta aatggagtgt cctcttccca gttttcgcaa
     1381 tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag
     1441 ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca
     1501 tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg
     1561 ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc
     1621 tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca
     1681 taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc
     1741 accagcttat ataccttagc aggagacatt ccttccgtat cttttgcgca gcggtatttt
     1801 tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatactctt cctttttcaa
     1861 tattattgaa gcatttatca aggttattgt ctcatgagcg gatacatatt tgaatgtatt
     1921 tagaaaaata aacaaatagg ggttccgcgc acgaattggc cagcgctgcc atttttgggg
     1981 tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggagctcg gtgccttgat
     2041 gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg
     2101 gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg
     2161 ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct
     2221 ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt
     2281 tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt
     2341 tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg
     2401 ggttcccaat gtacgtgcta tccacaggaa agaagctttt tcgacctttt tcccctgcta
     2461 gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga
     2521 tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat
     2581 cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga
     2641 actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct
     2701 tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa
     2761 agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca
     2821 tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct
     2881 tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct
     2941 tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt
     3001 cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg
     3061 gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg
     3121 ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg
     3181 gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc
     3241 gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg
     3301 tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct
     3361 tcctaatcga cgacgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag
     3421 cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg
     3481 cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg
     3541 ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt
     3601 gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat
     3661 ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc
     3721 cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt
     3781 attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg
     3841 tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc
     3901 tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg
     3961 tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc
     4021 ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt
     4081 gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg
     4141 cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc
     4201 ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac
     4261 cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag
     4321 cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc
     4381 gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc
     4441 gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc
     4501 ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag
     4561 atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt
     4621 catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca
     4681 tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct
     4741 tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt
     4801 tcatgcagcc gcacggctga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
     4861 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg
     4921 cttagacaac ttaataacac attgcggacg tttttaatgt actggggtgg atgcagtggg
     4981 ccccactctg tgtgccgaat tcggatccgg agagcggaga attaagggag tcacgttatg
     5041 acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacaga accgcaacgt
     5101 tgaaggagcc actcagccgc gggtttctgg agtttaatga gctaagcaca tacgtcagaa
     5161 accattattg cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt
     5221 caaaaatgct ccactgacgt tccataaatt cccctcggta tccaattaga gtctcatatt
     5281 cactctcaat ccaaataatc tgcaccggat ctggatcgtt tcgcatgatt gaacaagatg
     5341 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     5401 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     5461 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actccaggac gaggcagcgc
     5521 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     5581 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     5641 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     5701 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     5761 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     5821 cgccagccga actgttcgcc aggctcaagg cgcgtatgcc cgacggcgag gatctcgtcg
     5881 tgactcatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     5941 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     6001 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     6061 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     6121 cgggtctgga tctagagtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga
     6181 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
     6241 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
     6301 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
     6361 aaattatcgc gcgcggtgtc atctatgtta ctagatcgac gctgcaagaa ttcaagcttg
     6421 gaggaattcc aatcccacaa aaatctgagc ttaacagcac agttgctcct ctcagagcag
     6481 aatcgggtat tcaacaccct catatcaact actacgttgt gtataacggt ccacatgccg
     6541 gtatatacga tgactggggt tgtacaaagg cggcaacaaa cggcgttccc ggagttgcac
     6601 acaagaaatt tgccactatt acagaggcaa gagcagcagc tgacgcgtac acaacaagtc
     6661 agcaaacaga caggttgaac ttcatcccca aaggagaagc tcaactcaag cccaagagct
     6721 ttgctaaggc cctaacaagc ccaccaaagc aaaaagccca ctggctcacg ctaggaacca
     6781 aaaggcccag cagtgatcca gccccaaaag agatctcctt tgccccggag attacaatgg
     6841 acgatttcct ctatctttac gatctaggaa ggaagttcga aggtgaaggt gacgacacta
     6901 tgttcaccac tgataatgag aaggttagcc tcttcaattt cagaaagaat gctgacccac
     6961 agatggttag agaggcctac gcagcaagtc tcatcaagac gatctacccg agtaacaatc
     7021 tccaggagat caaatacctt cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta
     7081 attgcatcaa gaacacagag aaagacatat ttctcaagat cagaagtact attccagtat
     7141 ggacgattca aggcttgctt cataaaccaa ggcaagtaat agagattgga gtctctaaaa
     7201 aggtagttcc tactgaatct aaggccatgc atggagtcta agattcaaat cgaggatcta
     7261 acagaactcg ccgtcaagac tggcgaacag ttcatacaga gtcttttacg actcaatgac
     7321 aagaagaaaa tcttcgtcaa catggtggag cacgacactc tggtctactc caaaaatgtc
     7381 aaagatacag tctcagaaga tcaaagggct attgagactt ttcaacaaag gataatttcg
     7441 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa
     7501 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat
     7561 ctctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
     7621 gaagaggttc caaccacgtc tacaaagcaa gtggattgat gtgacatctc cactgacgta
     7681 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
     7741 tttcatttgg agaggacacg ctcgagtata agagctcatt tttacaacaa ttaccaacaa
     7801 caacaaacaa caaacaacat tacaattaca tttacaatta tcgatacaat ggtataagag
     7861 ctcattttta caacaattac caacaacaac aaacaacaaa caacattaca attacattta
     7921 caattatcga tacatggctt ctagcccacc gaagaagaag cggaaggtca gctggaaaaa
     7981 gcttatggtt agcaaaggtg aagaactgtt caccggtgtt gtgccgatcc tggttgaact
     8041 ggacggtgac gttaacggtc acaaattctc tgtttccggt gaaggtgaag gtgacgctac
     8101 ctacggtaaa ctgaccctga aattcatctc taccaccggt aaactgccgg ttccgtggcc
     8161 gaccctggtg accaccctga cctacggtgt tcagtgcttc tcccgttacc cggaccacat
     8221 gaaacagcac gatttcttca aatccgcgat gccggaaggt tacgtgcagg aacgtaccat
     8281 cttcttcaaa gatgacggta actacaaaac ccgtgctgaa gttaaattcg aaggtgacac
     8341 cctggttaac cgtattgaac tgaaaggcat cgacttcaaa gaagatggta acatcctggg
     8401 tcacaaactg gaatacaact acaactgtca caacgtttac atcatggcgg ataaacagaa
     8461 aaacggtatc aaagttaact tcaaaatccg tcacaacatc gaagatggtt ctgttcagct
     8521 ggctgatcac taccagcaga acaccccgat cggtgacggt ccggttctgc tgccggacaa
     8581 ccactacctg tctacctgct ccgcgctgag caaagacccg aacgaaaaac gtgaccacat
     8641 ggttctgctg gaattcgtta ccgcggcggg tatcaccctg ggcatggatg aactgtacaa
     8701 aacctccggc ggttctggtg gcggtggctc tggtggtggt ggttctggtg gtggcggctc
     8761 tggtggtggt ggttctggtg gcggtggttc tggcggcaaa ttcgacatca gcgaattcta
     8821 caaactggct ccggttgaca aaaaaggtca gccgttcccg ttcgaccagc tgaatggtaa
     8881 agtggtgctg atcgttaacg ttgcatctaa atgcggtttc accccgcagt acaaagaact
     8941 ggaagcgctg tataaacgtt acaaagatga aggtttcact atcatcggct tcccgtgtaa
     9001 ccagttcggt caccaggaac cgggctctga tgaagaaatc ctgaactctc tgaaatacgt
     9061 tcgtccgggt ggtggtttcg aaccgaactt cccgatcatg aaaaagatcg acgttaacgg
     9121 tggtaacgaa gatccggttt ataaattcct gcgtgaagcg ctgccggctc cgtctgatga
     9181 cgcgaccgcg ctgatgaccg atccgaaact gatcacctgg agcccggttt gccgtaacga
     9241 tgttgcgtgg aacttcgaaa aattcctggt tgacaaaaaa ggtaaagttt acgaacgtta
     9301 tagctctctg accaaaccgt cctctctgtc tgaaaccatc gaagaactgc tgaaagaagt
     9361 tgaataagct tgtcctgctt taatgagata tgcgagaagc ctatgatcgc atgatatttg
     9421 ctttcaattc tgttgtgcac gttgtaaaaa acctgagcat gtgtagctca gatccttacc
     9481 gccggtttcg gttcattcta atgaatatat cacccgttac tatcgtattt ttatgaataa
     9541 tattctccgt tcaatttact gattgtaccc tactacttat atgtacaata ttaaaatgaa
     9601 aacaatatat tgtgctgaat aggtttatag cgacatctat gatagagcgc cacaataaca
     9661 aacaattgcg ttttattatt acaaatccaa ttttaaaaaa agcggcagaa ccggtcaaac
     9721 ctaaaagact gattacataa atcttattca aatttcaaaa gtgccccagg ggctagtatc
     9781 tacgacacac cgagcggcga actaataacg ctcactgaag ggaactccgg ttccccgccg
     9841 gcgcgcatgg gtgagattcc ttgaagttga gtattggccg tccgctctac cgaaagttac
     9901 gggcaccatt caacccggtc cagcacggcg gccgggtaac cgacttgctg ccccgagaat
     9961 tatgcagcat ttttttggtg tatgtgggcc ccaaatgaag t
//
