LOCUS       sequence-378856-        9938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a 
            nuclear-targeted control roGFP2 fusion not responsive to peroxides 
            used alongside plasmids 191759 and 191758.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9938)
  AUTHORS   Exposito-Rodriguez M, Reeder B, Brooke GN, Hough MA, Laissue PP, 
            Mullineaux PM
  TITLE     A novel glutathione peroxidase-based biosensor disentangles 
            differential subcellular accumulation of H2O2 and lipid 
            hydroperoxides
  JOURNAL   bioRxiv 2024.01.18.576236
REFERENCE   2  (bases 1 to 9938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.18.576236"
COMMENT     Sequence Label: pICH-nuc-roGFP2-GPXC71S
FEATURES             Location/Qualifiers
     source          1..9938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      705..1412
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    1475..1499
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1622..2210)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1702..1721)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2381..3175)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(3176..3280)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3563..3757
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(3823..4896)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5325..5954)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6218..6242
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        6420..6599
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        6452..6635
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             6656..7450
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6710..6729)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7320..7339
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      7476..7728
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        8744..9089
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     9032..9052
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             9280..9300
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtctaaagac ccgaacgaaa aacgtgatca catggttctg ctggaattcg ttaccgcagc
       61 gggcatcacc ctgggtatgg acgaactgta caaaacttct ggtggttctg gcggtggcgg
      121 ctctggcggt ggtggtagcg gcggcggtgg ttctggtggc ggtggttccg gtggcggcgg
      181 tagcggtggc gaattcgacc agtcttctta ctcttccatc taccacatct ctgttaaaga
      241 catcgatggc aacgacgtgt ctctgtctaa attcaccggt aaagtgctgc tgatcgtaaa
      301 cgttgcgtct aaatccggtc tgacccaggg taactacaaa gaactgaaca tcctgtacgc
      361 gaaatacaaa accaaaggtc tggaaatcct ggcgttcccg tgtaaccagt tcggttctca
      421 ggaaccgggt tctaacaaag aaattaaaga taacatctgt accaccttta aaggtgaatt
      481 cccgatcttt gataaaattg aggttaacgg tgaaaacgcg tccccactgt ataaatttct
      541 gaaagagcag aaaggcggtc tgttcggtga tagcattaaa tggaactttg ctaaattctt
      601 agttgataaa caaggtaacg tagttgatcg tttcgcgccg accactagcc cgttagagat
      661 tgagaaagac attgaaaaac tgctggcatc tacctaagct tgtcctgctt taatgagata
      721 tgcgagaagc ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa
      781 acctgagcat gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat
      841 cacccgttac tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtaccc
      901 tactacttat atgtacaata ttaaaatgaa aacaatatat tgtgctgaat aggtttatag
      961 cgacatctat gatagagcgc cacaataaca aacaattgcg ttttattatt acaaatccaa
     1021 ttttaaaaaa agcggcagaa ccggtcaaac ctaaaagact gattacataa atcttattca
     1081 aatttcaaaa gtgccccagg ggctagtatc tacgacacac cgagcggcga actaataacg
     1141 ctcactgaag ggaactccgg ttccccgccg gcgcgcatgg gtgagattcc ttgaagttga
     1201 gtattggccg tccgctctac cgaaagttac gggcaccatt caacccggtc cagcacggcg
     1261 gccgggtaac cgacttgctg ccccgagaat tatgcagcat ttttttggtg tatgtgggcc
     1321 ccaaatgaag tgcaggtcaa accttgacag tgacgacaaa tcgttgggcg ggtccagggc
     1381 gaattttgcg acaacatgtc gaggctcagc aggaccgcta ctagaggatg cacatgtgac
     1441 cgagggacac gaagtggttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     1501 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     1561 ccgttcgtcc atttgtatgt gccaaccaca ggaaccgtaa aaaggccgcg ttgctggcgt
     1621 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1681 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1741 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1801 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1861 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1921 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1981 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2041 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     2101 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2161 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2221 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2281 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2341 aatcaatcta aagtatatat gagtaaactt ggtctgacag ctaaaacaat tcatccagta
     2401 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct
     2461 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat
     2521 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt
     2581 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct
     2641 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcctcttccc
     2701 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc
     2761 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga
     2821 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg
     2881 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa
     2941 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc
     3001 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt
     3061 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttgcgc
     3121 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatactct
     3181 tcctttttca atattattga agcatttatc aaggttattg tctcatgagc ggatacatat
     3241 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacgaattgg ccagcgctgc
     3301 catttttggg gtgaggccgt tcgcggccga ggggcgcagc ccctgggggg atgggagctc
     3361 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg
     3421 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg
     3481 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc
     3541 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag
     3601 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat
     3661 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat
     3721 gtacggcttt gggttcccaa tgtacgtgct atccacagga aagaagcttt ttcgaccttt
     3781 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc
     3841 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc
     3901 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca
     3961 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct
     4021 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg
     4081 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat
     4141 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct
     4201 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc
     4261 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt
     4321 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc
     4381 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt
     4441 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc
     4501 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac
     4561 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg
     4621 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc
     4681 cttgggcggc ttcctaatcg acgacgcacc ggctgccggc ggttgccggg attctttgcg
     4741 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg
     4801 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc
     4861 gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt
     4921 ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt
     4981 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg
     5041 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag
     5101 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat
     5161 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt
     5221 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact
     5281 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat
     5341 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca
     5401 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac
     5461 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg
     5521 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg
     5581 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc
     5641 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg
     5701 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc
     5761 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg
     5821 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg
     5881 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca
     5941 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg
     6001 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc
     6061 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac
     6121 cggccaggat ttcatgcagc cgcacggctg atgggctgcc tgtatcgagt ggtgattttg
     6181 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
     6241 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactggggtg
     6301 gatgcagtgg gccccactct gtgtgccgaa ttcggatccg gagagcggag aattaaggga
     6361 gtcacgttat gacccccgcc gatgacgcgg gacaagccgt tttacgtttg gaactgacag
     6421 aaccgcaacg ttgaaggagc cactcagccg cgggtttctg gagtttaatg agctaagcac
     6481 atacgtcaga aaccattatt gcgcgttcaa aagtcgccta aggtcactat cagctagcaa
     6541 atatttcttg tcaaaaatgc tccactgacg ttccataaat tcccctcggt atccaattag
     6601 agtctcatat tcactctcaa tccaaataat ctgcaccgga tctggatcgt ttcgcatgat
     6661 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6721 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6781 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactccagga
     6841 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     6901 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     6961 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     7021 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     7081 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     7141 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgtatgc ccgacggcga
     7201 ggatctcgtc gtgactcatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     7261 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     7321 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     7381 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7441 gttcttctga gcgggtctgg atctagagtc aagcagatcg ttcaaacatt tggcaataaa
     7501 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     7561 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     7621 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     7681 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga cgctgcaaga
     7741 attcaagctt ggaggaattc caatcccaca aaaatctgag cttaacagca cagttgctcc
     7801 tctcagagca gaatcgggta ttcaacaccc tcatatcaac tactacgttg tgtataacgg
     7861 tccacatgcc ggtatatacg atgactgggg ttgtacaaag gcggcaacaa acggcgttcc
     7921 cggagttgca cacaagaaat ttgccactat tacagaggca agagcagcag ctgacgcgta
     7981 cacaacaagt cagcaaacag acaggttgaa cttcatcccc aaaggagaag ctcaactcaa
     8041 gcccaagagc tttgctaagg ccctaacaag cccaccaaag caaaaagccc actggctcac
     8101 gctaggaacc aaaaggccca gcagtgatcc agccccaaaa gagatctcct ttgccccgga
     8161 gattacaatg gacgatttcc tctatcttta cgatctagga aggaagttcg aaggtgaagg
     8221 tgacgacact atgttcacca ctgataatga gaaggttagc ctcttcaatt tcagaaagaa
     8281 tgctgaccca cagatggtta gagaggccta cgcagcaagt ctcatcaaga cgatctaccc
     8341 gagtaacaat ctccaggaga tcaaatacct tcccaagaag gttaaagatg cagtcaaaag
     8401 attcaggact aattgcatca agaacacaga gaaagacata tttctcaaga tcagaagtac
     8461 tattccagta tggacgattc aaggcttgct tcataaacca aggcaagtaa tagagattgg
     8521 agtctctaaa aaggtagttc ctactgaatc taaggccatg catggagtct aagattcaaa
     8581 tcgaggatct aacagaactc gccgtcaaga ctggcgaaca gttcatacag agtcttttac
     8641 gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacact ctggtctact
     8701 ccaaaaatgt caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa
     8761 ggataatttc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa
     8821 ggacagtaga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta
     8881 tcattcaaga tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     8941 tcgtggaaaa agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct
     9001 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     9061 aaggaagttc atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca
     9121 attaccaaca acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa
     9181 tggtataaga gctcattttt acaacaatta ccaacaacaa caaacaacaa acaacattac
     9241 aattacattt acaattatcg atacatggct tctagcccac cgaagaagaa gcggaaggtc
     9301 agctggaaaa agcttatggt ttctaaaggt gaagaactgt tcaccggtgt tgttccgatc
     9361 ctggttgaac tggatggtga cgttaacggt cacaaattct ctgtttctgg tgaaggtgaa
     9421 ggtgatgcta cctacggtaa actgaccctg aaattcatct ccaccactgg taaactgccg
     9481 gtgccgtggc cgaccctggt taccaccctg acctacggcg ttcagtgctt ctctcgttac
     9541 ccggaccaca tgaaacagca cgacttcttc aaatccgcta tgccggaagg ctacgttcag
     9601 gaacgcacca ttttcttcaa agatgacggt aactacaaaa cccgcgctga agttaaattc
     9661 gaaggtgata ctctggttaa ccgtatcgaa ctgaaaggta tcgatttcaa agaagacggt
     9721 aacatcctgg gtcacaaact ggaatacaac tacaactgcc acaacgtgta catcatggcg
     9781 gacaaacaga aaaacggtat taaagttaac ttcaaaatcc gtcacaacat cgaagatggc
     9841 agcgtgcagc tggccgacca ctaccagcag aacaccccga tcggtgacgg tccggttctg
     9901 ctgccggaca accactacct gtccacctgc tccgcgct
//
