LOCUS       sequence-378858-        9929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ti plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide 
            detection in the nuclei of plant cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Exposito-Rodriguez M, Reeder B, Brooke GN, Hough MA, Laissue PP, 
            Mullineaux PM
  TITLE     A novel glutathione peroxidase-based biosensor disentangles 
            differential subcellular accumulation of H2O2 and lipid 
            hydroperoxides
  JOURNAL   bioRxiv 2024.01.18.576236
REFERENCE   2  (bases 1 to 9929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.18.576236"
COMMENT     Sequence Label: pICH-nuc-roGFP2-ORP1
FEATURES             Location/Qualifiers
     source          1..9929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      622..1329
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    1392..1416
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1539..2127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1619..1638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2298..3092)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(3093..3197)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3480..3674
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(3740..4813)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5242..5871)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6135..6159
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        6337..6516
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        6369..6552
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             6573..7367
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6627..6646)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7237..7256
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      7393..7645
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        8661..9006
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     8949..8969
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             9197..9217
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcatggatga actgtacaaa acctccggcg gttctggtgg cggtggctct ggtggtggtg
       61 gttctggtgg tggcggctct ggtggtggtg gttctggtgg cggtggttct ggcggcgaat
      121 tcgacatcag cgaattctac aaactggctc cggttgacaa aaaaggtcag ccgttcccgt
      181 tcgaccagct gaaaggtaaa gtggtgctga tcgttaacgt tgcatctaaa tgcggtttca
      241 ccccgcagta caaagaactg gaagcgctgt ataaacgtta caaagatgaa ggtttcacta
      301 tcatcggctt cccgtgtaac cagttcggtc accaggaacc gggctctgat gaagaaatcg
      361 cccagttctg ccaactgaac tatggcgtga ctttcccgat catgaaaaag atcgacgtta
      421 acggtggtaa cgaagatccg gtttataaat tcctgaagag ccaaaaatcc ggtatgttgg
      481 gcttgagagg tatcaaatgg aacttcgaaa aattcctggt tgacaaaaaa ggtaaagttt
      541 acgaacgtta tagctctctg accaaaccgt cctctctgtc tgaaaccatc gaagaactgc
      601 tgaaagaagt ttaagcttgt cctgctttaa tgagatatgc gagaagccta tgatcgcatg
      661 atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat
      721 ccttaccgcc ggtttcggtt cattctaatg aatatatcac ccgttactat cgtattttta
      781 tgaataatat tctccgttca atttactgat tgtaccctac tacttatatg tacaatatta
      841 aaatgaaaac aatatattgt gctgaatagg tttatagcga catctatgat agagcgccac
      901 aataacaaac aattgcgttt tattattaca aatccaattt taaaaaaagc ggcagaaccg
      961 gtcaaaccta aaagactgat tacataaatc ttattcaaat ttcaaaagtg ccccaggggc
     1021 tagtatctac gacacaccga gcggcgaact aataacgctc actgaaggga actccggttc
     1081 cccgccggcg cgcatgggtg agattccttg aagttgagta ttggccgtcc gctctaccga
     1141 aagttacggg caccattcaa cccggtccag cacggcggcc gggtaaccga cttgctgccc
     1201 cgagaattat gcagcatttt tttggtgtat gtgggcccca aatgaagtgc aggtcaaacc
     1261 ttgacagtga cgacaaatcg ttgggcgggt ccagggcgaa ttttgcgaca acatgtcgag
     1321 gctcagcagg accgctacta gaggatgcac atgtgaccga gggacacgaa gtggtttaaa
     1381 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     1441 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgcc
     1501 aaccacagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1561 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1621 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1681 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1741 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1801 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1861 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1921 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     1981 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2041 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2101 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2161 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2221 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2281 taaacttggt ctgacagcta aaacaattca tccagtaaaa tataatattt tattttctcc
     2341 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata
     2401 tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt
     2461 ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc
     2521 aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac
     2581 agctcgcgcg gatctttaaa tggagtgtcc tcttcccagt tttcgcaatc cacatcggcc
     2641 agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag
     2701 ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata
     2761 atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca
     2821 ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc
     2881 agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct
     2941 ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat
     3001 accttagcag gagacattcc ttccgtatct tttgcgcagc ggtatttttc gatcagtttt
     3061 ttcaattccg gtgatattct cattttagcc atactcttcc tttttcaata ttattgaagc
     3121 atttatcaag gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3181 caaatagggg ttccgcgcac gaattggcca gcgctgccat ttttggggtg aggccgttcg
     3241 cggccgaggg gcgcagcccc tggggggatg ggagctcggt gccttgatgt gggcgccggc
     3301 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
     3361 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
     3421 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
     3481 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
     3541 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
     3601 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
     3661 acgtgctatc cacaggaaag aagctttttc gacctttttc ccctgctagg gcaatttgcc
     3721 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
     3781 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
     3841 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
     3901 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
     3961 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
     4021 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
     4081 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
     4141 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
     4201 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
     4261 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
     4321 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
     4381 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
     4441 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
     4501 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
     4561 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
     4621 acgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
     4681 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
     4741 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
     4801 tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca
     4861 gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac
     4921 ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca
     4981 tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca
     5041 gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg
     5101 ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg
     5161 cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca
     5221 ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg
     5281 gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc
     5341 agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc
     5401 ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac
     5461 aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag
     5521 gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc
     5581 tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa
     5641 gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac
     5701 ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga
     5761 atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat
     5821 ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc
     5881 ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg
     5941 ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag
     6001 ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc
     6061 acggctgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
     6121 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
     6181 aataacacat tgcggacgtt tttaatgtac tggggtggat gcagtgggcc ccactctgtg
     6241 tgccgaattc ggatccggag agcggagaat taagggagtc acgttatgac ccccgccgat
     6301 gacgcgggac aagccgtttt acgtttggaa ctgacagaac cgcaacgttg aaggagccac
     6361 tcagccgcgg gtttctggag tttaatgagc taagcacata cgtcagaaac cattattgcg
     6421 cgttcaaaag tcgcctaagg tcactatcag ctagcaaata tttcttgtca aaaatgctcc
     6481 actgacgttc cataaattcc cctcggtatc caattagagt ctcatattca ctctcaatcc
     6541 aaataatctg caccggatct ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     6601 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     6661 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     6721 agaccgacct gtccggtgcc ctgaatgaac tccaggacga ggcagcgcgg ctatcgtggc
     6781 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     6841 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     6901 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     6961 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     7021 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     7081 tgttcgccag gctcaaggcg cgtatgcccg acggcgagga tctcgtcgtg actcatggcg
     7141 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     7201 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     7261 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     7321 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggtctggatc
     7381 tagagtcaag cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     7441 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     7501 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     7561 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     7621 gcggtgtcat ctatgttact agatcgacgc tgcaagaatt caagcttgga ggaattccaa
     7681 tcccacaaaa atctgagctt aacagcacag ttgctcctct cagagcagaa tcgggtattc
     7741 aacaccctca tatcaactac tacgttgtgt ataacggtcc acatgccggt atatacgatg
     7801 actggggttg tacaaaggcg gcaacaaacg gcgttcccgg agttgcacac aagaaatttg
     7861 ccactattac agaggcaaga gcagcagctg acgcgtacac aacaagtcag caaacagaca
     7921 ggttgaactt catccccaaa ggagaagctc aactcaagcc caagagcttt gctaaggccc
     7981 taacaagccc accaaagcaa aaagcccact ggctcacgct aggaaccaaa aggcccagca
     8041 gtgatccagc cccaaaagag atctcctttg ccccggagat tacaatggac gatttcctct
     8101 atctttacga tctaggaagg aagttcgaag gtgaaggtga cgacactatg ttcaccactg
     8161 ataatgagaa ggttagcctc ttcaatttca gaaagaatgc tgacccacag atggttagag
     8221 aggcctacgc agcaagtctc atcaagacga tctacccgag taacaatctc caggagatca
     8281 aataccttcc caagaaggtt aaagatgcag tcaaaagatt caggactaat tgcatcaaga
     8341 acacagagaa agacatattt ctcaagatca gaagtactat tccagtatgg acgattcaag
     8401 gcttgcttca taaaccaagg caagtaatag agattggagt ctctaaaaag gtagttccta
     8461 ctgaatctaa ggccatgcat ggagtctaag attcaaatcg aggatctaac agaactcgcc
     8521 gtcaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa gaagaaaatc
     8581 ttcgtcaaca tggtggagca cgacactctg gtctactcca aaaatgtcaa agatacagtc
     8641 tcagaagatc aaagggctat tgagactttt caacaaagga taatttcggg aaacctcctc
     8701 ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc
     8761 tcctacaaat gccatcattg cgataaagga aaggctatca ttcaagatct ctctgccgac
     8821 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agaggttcca
     8881 accacgtcta caaagcaagt ggattgatgt gacatctcca ctgacgtaag ggatgacgca
     8941 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
     9001 aggacacgct cgagtataag agctcatttt tacaacaatt accaacaaca acaaacaaca
     9061 aacaacatta caattacatt tacaattatc gatacaatgg tataagagct catttttaca
     9121 acaattacca acaacaacaa acaacaaaca acattacaat tacatttaca attatcgata
     9181 catggcttct agcccaccga agaagaagcg gaaggtcagc tggaaaaagc ttatggttag
     9241 caaaggtgaa gaactgttca ccggtgttgt gccgatcctg gttgaactgg acggtgacgt
     9301 taacggtcac aaattctctg tttccggtga aggtgaaggt gacgctacct acggtaaact
     9361 gaccctgaaa ttcatctcta ccaccggtaa actgccggtt ccgtggccga ccctggtgac
     9421 caccctgacc tacggtgttc agtgcttctc ccgttacccg gaccacatga aacagcacga
     9481 tttcttcaaa tccgcgatgc cggaaggtta cgtgcaggaa cgtaccatct tcttcaaaga
     9541 tgacggtaac tacaaaaccc gtgctgaagt taaattcgaa ggtgacaccc tggttaaccg
     9601 tattgaactg aaaggcatcg acttcaaaga agatggtaac atcctgggtc acaaactgga
     9661 atacaactac aactgtcaca acgtttacat catggcggat aaacagaaaa acggtatcaa
     9721 agttaacttc aaaatccgtc acaacatcga agatggttct gttcagctgg ctgatcacta
     9781 ccagcagaac accccgatcg gtgacggtcc ggttctgctg ccggacaacc actacctgtc
     9841 tacctgctcc gcgctgagca aagacccgaa cgaaaaacgt gaccacatgg ttctgctgga
     9901 attcgttacc gcggcgggta tcaccctgg
//
