LOCUS       sequence-378859-       10071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted 
            control roGFP2 fusion not responsive to peroxides used alongside 
            plasmids 191699 and 191701.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10071)
  AUTHORS   Exposito-Rodriguez M, Reeder B, Brooke GN, Hough MA, Laissue PP, 
            Mullineaux PM
  TITLE     A novel glutathione peroxidase-based biosensor disentangles 
            differential subcellular accumulation of H2O2 and lipid 
            hydroperoxides
  JOURNAL   bioRxiv 2024.01.18.576236
REFERENCE   2  (bases 1 to 10071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.18.576236"
COMMENT     Sequence Label: pICH-chl-roGFP2-GPXC71S
FEATURES             Location/Qualifiers
     source          1..10071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..65
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(188..776)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(268..287)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(947..1741)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(1742..1846)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2129..2323
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2389..3462)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3891..4520)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4784..4808
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4986..5165
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        5018..5201
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5222..6016
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5276..5295)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5886..5905
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6042..6294
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7310..7655
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     7598..7618
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      9342..10049
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 tgtgaccgag ggacacgaag tggtttaaac tatcagtgtt tgacaggata tattggcggg
       61 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
      121 gtttatccgt tcgtccattt gtatgtgcca accacaggaa ccgtaaaaag gccgcgttgc
      181 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      241 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      301 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      361 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      421 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      481 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      541 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      601 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
      661 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      721 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      781 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      841 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      901 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctaa aacaattcat
      961 ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa
     1021 atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa
     1081 tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc
     1141 catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt
     1201 cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtcct
     1261 cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg
     1321 ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga
     1381 gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact
     1441 cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc
     1501 gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct
     1561 tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata
     1621 ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt
     1681 ttgcgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca
     1741 tactcttcct ttttcaatat tattgaagca tttatcaagg ttattgtctc atgagcggat
     1801 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcacg aattggccag
     1861 cgctgccatt tttggggtga ggccgttcgc ggccgagggg cgcagcccct ggggggatgg
     1921 gagctcggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
     1981 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
     2041 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
     2101 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
     2161 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
     2221 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
     2281 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga agctttttcg
     2341 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
     2401 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
     2461 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
     2521 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
     2581 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
     2641 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
     2701 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
     2761 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
     2821 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
     2881 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
     2941 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
     3001 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
     3061 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
     3121 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
     3181 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
     3241 gtcgcccttg ggcggcttcc taatcgacga cgcaccggct gccggcggtt gccgggattc
     3301 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
     3361 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
     3421 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
     3481 ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg
     3541 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
     3601 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
     3661 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
     3721 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
     3781 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
     3841 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
     3901 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
     3961 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
     4021 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
     4081 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
     4141 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
     4201 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
     4261 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
     4321 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
     4381 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
     4441 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
     4501 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
     4561 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
     4621 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
     4681 acaaaccggc caggatttca tgcagccgca cggctgatgg gctgcctgta tcgagtggtg
     4741 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg
     4801 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact
     4861 ggggtggatg cagtgggccc cactctgtgt gccgaattcg gatccggaga gcggagaatt
     4921 aagggagtca cgttatgacc cccgccgatg acgcgggaca agccgtttta cgtttggaac
     4981 tgacagaacc gcaacgttga aggagccact cagccgcggg tttctggagt ttaatgagct
     5041 aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc
     5101 tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc
     5161 aattagagtc tcatattcac tctcaatcca aataatctgc accggatctg gatcgtttcg
     5221 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     5281 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     5341 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5401 ccaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5461 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5521 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5581 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5641 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5701 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gtatgcccga
     5761 cggcgaggat ctcgtcgtga ctcatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5821 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5881 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5941 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     6001 tgacgagttc ttctgagcgg gtctggatct agagtcaagc agatcgttca aacatttggc
     6061 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
     6121 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     6181 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     6241 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgacgct
     6301 gcaagaattc aagcttggag gaattccaat cccacaaaaa tctgagctta acagcacagt
     6361 tgctcctctc agagcagaat cgggtattca acaccctcat atcaactact acgttgtgta
     6421 taacggtcca catgccggta tatacgatga ctggggttgt acaaaggcgg caacaaacgg
     6481 cgttcccgga gttgcacaca agaaatttgc cactattaca gaggcaagag cagcagctga
     6541 cgcgtacaca acaagtcagc aaacagacag gttgaacttc atccccaaag gagaagctca
     6601 actcaagccc aagagctttg ctaaggccct aacaagccca ccaaagcaaa aagcccactg
     6661 gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc ccaaaagaga tctcctttgc
     6721 cccggagatt acaatggacg atttcctcta tctttacgat ctaggaagga agttcgaagg
     6781 tgaaggtgac gacactatgt tcaccactga taatgagaag gttagcctct tcaatttcag
     6841 aaagaatgct gacccacaga tggttagaga ggcctacgca gcaagtctca tcaagacgat
     6901 ctacccgagt aacaatctcc aggagatcaa ataccttccc aagaaggtta aagatgcagt
     6961 caaaagattc aggactaatt gcatcaagaa cacagagaaa gacatatttc tcaagatcag
     7021 aagtactatt ccagtatgga cgattcaagg cttgcttcat aaaccaaggc aagtaataga
     7081 gattggagtc tctaaaaagg tagttcctac tgaatctaag gccatgcatg gagtctaaga
     7141 ttcaaatcga ggatctaaca gaactcgccg tcaagactgg cgaacagttc atacagagtc
     7201 ttttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacactctgg
     7261 tctactccaa aaatgtcaaa gatacagtct cagaagatca aagggctatt gagacttttc
     7321 aacaaaggat aatttcggga aacctcctcg gattccattg cccagctatc tgtcacttca
     7381 tcgaaaggac agtagaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa
     7441 aggctatcat tcaagatctc tctgccgaca gtggtcccaa agatggaccc ccacccacga
     7501 ggagcatcgt ggaaaaagaa gaggttccaa ccacgtctac aaagcaagtg gattgatgtg
     7561 acatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct
     7621 ctatataagg aagttcattt catttggaga ggacacgctc gagtataaga gctcattttt
     7681 acaacaatta ccaacaacaa caaacaacaa acaacattac aattacattt acaattatcg
     7741 atacaatggt ataagagctc atttttacaa caattaccaa caacaacaaa caacaaacaa
     7801 cattacaatt acatttacaa ttatcgataa catggcttct tctatgcttt cttctgctgc
     7861 tgttgttgct actcgtgcta gtgctgctca agctagtatg gttgctcctt ttactggact
     7921 taagtctgct gcttcttttc ctgttactag aaagcaaaac aaccttgata ttacttctat
     7981 tgctagtaac ggaggaagag ttcgagcaaa gcttatggtt agcaaaggtg aagaactgtt
     8041 caccggtgtt gttccgatcc tggttgaact ggatggtgac gttaacggtc acaaattctc
     8101 tgtttctggt gaaggtgaag gtgatgctac ctacggtaaa ctgaccctga aattcatctc
     8161 caccactggt aaactgccgg tgccgtggcc gaccctggtt accaccctga cctacggcgt
     8221 tcagtgcttc tctcgttacc cggaccacat gaaacagcac gacttcttca aatccgctat
     8281 gccggaaggc tacgttcagg aacgcaccat tttcttcaaa gatgacggta actacaaaac
     8341 ccgcgctgaa gttaaattcg aaggtgatac tctggttaac cgtatcgaac tgaaaggtat
     8401 cgatttcaaa gaagacggta acatcctggg tcacaaactg gaatacaact acaactgcca
     8461 caacgtgtac atcatggcgg acaaacagaa aaacggtatt aaagttaact tcaaaatccg
     8521 tcacaacatc gaagatggca gcgtgcagct ggccgaccac taccagcaga acaccccgat
     8581 cggtgacggt ccggttctgc tgccggacaa ccactacctg tccacctgct ccgcgctgtc
     8641 taaagacccg aacgaaaaac gtgatcacat ggttctgctg gaattcgtta ccgcagcggg
     8701 catcaccctg ggtatggacg aactgtacaa aacttctggt ggttctggcg gtggcggctc
     8761 tggcggtggt ggtagcggcg gcggtggttc tggtggcggt ggttccggtg gcggcggtag
     8821 cggtggcgaa ttcgaccagt cttcttactc ttccatctac cacatctctg ttaaagacat
     8881 cgatggcaac gacgtgtctc tgtctaaatt caccggtaaa gtgctgctga tcgtaaacgt
     8941 tgcgtctaaa tccggtctga cccagggtaa ctacaaagaa ctgaacatcc tgtacgcgaa
     9001 atacaaaacc aaaggtctgg aaatcctggc gttcccgtgt aaccagttcg gttctcagga
     9061 accgggttct aacaaagaaa ttaaagataa catctgtacc acctttaaag gtgaattccc
     9121 gatctttgat aaaattgagg ttaacggtga aaacgcgtcc ccactgtata aatttctgaa
     9181 agagcagaaa ggcggtctgt tcggtgatag cattaaatgg aactttgcta aattcttagt
     9241 tgataaacaa ggtaacgtag ttgatcgttt cgcgccgacc actagcccgt tagagattga
     9301 gaaagacatt gaaaaactgc tggcatctac ctaagcttgt cctgctttaa tgagatatgc
     9361 gagaagccta tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc
     9421 tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt cattctaatg aatatatcac
     9481 ccgttactat cgtattttta tgaataatat tctccgttca atttactgat tgtaccctac
     9541 tacttatatg tacaatatta aaatgaaaac aatatattgt gctgaatagg tttatagcga
     9601 catctatgat agagcgccac aataacaaac aattgcgttt tattattaca aatccaattt
     9661 taaaaaaagc ggcagaaccg gtcaaaccta aaagactgat tacataaatc ttattcaaat
     9721 ttcaaaagtg ccccaggggc tagtatctac gacacaccga gcggcgaact aataacgctc
     9781 actgaaggga actccggttc cccgccggcg cgcatgggtg agattccttg aagttgagta
     9841 ttggccgtcc gctctaccga aagttacggg caccattcaa cccggtccag cacggcggcc
     9901 gggtaaccga cttgctgccc cgagaattat gcagcatttt tttggtgtat gtgggcccca
     9961 aatgaagtgc aggtcaaacc ttgacagtga cgacaaatcg ttgggcgggt ccagggcgaa
    10021 ttttgcgaca acatgtcgag gctcagcagg accgctacta gaggatgcac a
//
