LOCUS       sequence-378870-        4520 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses codon-optimized full length SARS-CoV-2 XBB Spike.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4520)
  TITLE     Codon-optimized Spike variant expression construct
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAGGS SARS-CoV-2 XBB Spike
FEATURES             Location/Qualifiers
     source          1..4520
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(3880..3899)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3945..4000
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3999..4018)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4361..4377)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4361..4377)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4374..4396)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4385..4401
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4409..4415,4416..4433,4434..4439))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4454..4475
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gctcctgggc acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgccacc
       61 atgttcgtct tcctcgtcct cctccccctc gtctcctccc agtgcgtcaa cctcatcacc
      121 cgcacccaga gctacaccaa ctccttcacc cgcggcgtct actaccccga caaggtcttc
      181 cgctcctccg tcctccactc cacccaggac ctcttcctcc ccttcttctc caacgtcacc
      241 tggttccacg ccatccacgt ctccggcacc aacggcacca agcgcttcga caaccccgcc
      301 ctccccttca acgacggcgt ctacttcgcc tccaccgaga agtccaacat catccgcggc
      361 tggatcttcg gcaccaccct cgactccaag acccagtccc tcctcatcgt caacaacgcc
      421 accaacgtcg tcatcaaggt ctgcgagttc cagttctgca acgacccctt cctcgacgtc
      481 taccagaaga acaacaagtc ctggatggag tccgagttcc gcgtctactc ctccgccaac
      541 aactgcacct tcgagtacgt ctcccagccc ttcctcatgg acctcgaggg caaggagggc
      601 aacttcaaga acctccgcga gttcgtcttc aagaacatcg acggctactt caagatctac
      661 tccaagcaca cccccatcaa cctcgagcgc gacctccccc agggcttctc cgccctcgag
      721 cccctcgtcg acctccccat cggcatcaac atcacccgct tccagaccct cctcgccctc
      781 caccgctcct acctcacccc cggcgactcc tcctccggct ggaccgccgg cgccgccgcc
      841 tactacgtcg gctacctcca gccccgcacc ttcctcctca agtacaacga gaacggcacc
      901 atcaccgacg ccgtcgactg cgccctcgac cccctctccg agaccaagtg caccctcaag
      961 tccttcaccg tcgagaaggg catctaccag acctccaact tccgcgtcca gcccaccgag
     1021 tccatcgtcc gcttccccaa catcaccaac ctctgcccct tccacgaggt cttcaacgcc
     1081 accaccttcg cctccgtcta cgcctggaac cgcaagcgca tctccaactg cgtcgccgac
     1141 tactccgtca tctacaactt cgcccctttc ttcgccttca agtgctacgg cgtctccccc
     1201 accaagctca acgacctctg cttcaccaac gtctacgccg actccttcgt catccgcggc
     1261 aacgaggtca gccagatcgc ccccggccag accggcaaca tcgccgacta caactacaag
     1321 ctccccgacg acttcaccgg ctgcgtcatc gcctggaact ccaacaagct cgactccaag
     1381 cccagcggca actacaacta cctctaccgc ctcttccgca agtccaagct caagcccttc
     1441 gagcgcgaca tctccaccga gatctaccag gccggcaaca aaccctgcaa cggcgtcgcc
     1501 ggcagcaact gctacagccc cctccagtcc tacggcttca ggcccaccta cggcgtcggc
     1561 caccagccct accgcgtcgt cgtcctctcc ttcgagctcc tccacgcccc cgccaccgtc
     1621 tgcggcccca agaagtccac caacctcgtc aagaacaagt gcgtcaactt caacttcaac
     1681 ggcctcaccg gcaccggcgt cctcaccgag tccaacaaga agttcctccc cttccagcag
     1741 ttcggccgcg acatcgccga caccaccgac gccgtccgcg acccccagac cctcgagatc
     1801 ctcgacatca ccccctgctc cttcggcggc gtctccgtca tcacccccgg caccaacacc
     1861 tccaaccagg tcgccgtcct ctaccagggt gtcaactgca ccgaggtccc cgtcgccatc
     1921 cacgccgacc agctcacccc cacctggcgc gtctactcca ccggctccaa cgtcttccag
     1981 acccgcgccg gctgcctcat cggcgccgag tacgtcaaca actcctacga gtgcgacatc
     2041 cccatcggcg ccggcatctg tgcctcctac cagacccaga ccaagtccca ccgccgcgcc
     2101 cgctccgtcg cctcccagtc catcatcgcc tacaccatgt ccctcggcgc cgagaactcc
     2161 gtcgcctact ccaacaactc catcgccatc cccaccaact tcaccatctc cgtcaccacc
     2221 gagatcctcc ccgtctccat gaccaagacc tccgtcgact gcaccatgta catctgcggc
     2281 gactccaccg agtgctccaa cctcctcctc cagtacggct ccttctgcac ccagctcaag
     2341 cgcgccctca ccggcatcgc cgtcgagcag gacaagaaca cccaggaggt cttcgcccag
     2401 gtcaagcaga tctacaagac cccccccatc aagtacttcg gcggcttcaa cttctcccag
     2461 atcctccccg acccctccaa gccctccaag cgctccttca tcgaggacct cctcttcaac
     2521 aaggtcaccc tcgccgacgc cggcttcatc aagcagtacg gcgactgcct cggcgacatc
     2581 gccgcccgcg acctcatctg cgcccagaag ttcaacggcc tcaccgtcct cccccccctc
     2641 ctcaccgacg agatgatcgc ccagtacacc tccgccctcc tcgccggcac catcacctcc
     2701 ggctggacct tcggcgccgg cgccgccctc cagatcccct tcgccatgca gatggcctac
     2761 cgcttcaacg gcatcggcgt cacccagaac gtcctctacg agaaccagaa gctcatcgcc
     2821 aaccagttca actccgccat cggcaagatc caggactccc tctcctccac cgcctccgcc
     2881 ctcggcaagc tccaggacgt cgtcaaccac aacgcccagg ccctcaacac cctcgtcaag
     2941 cagctctcct ccaagttcgg cgccatctcc tccgtcctca acgacatcct gtcccgcctc
     3001 gacaaggtcg aggccgaggt ccagatcgac cgcctcatca ccggccgcct ccagtccctc
     3061 cagacctacg tcacccagca gctcatccgc gccgccgaga tccgcgcctc cgccaacctc
     3121 gccgccacca agatgtccga gtgcgtcctc ggccagtcca agcgcgtcga cttctgcggc
     3181 aagggctacc acctcatgtc cttcccccag tccgcccccc acggcgtcgt cttcctccac
     3241 gtcacctacg tccccgccca ggagaagaac ttcaccaccg cccccgccat ctgccacgac
     3301 ggcaaggccc acttcccccg cgagggcgtc ttcgtctcca acggcaccca ctggttcgtc
     3361 acccagcgca acttctacga gccccagatc atcaccaccg acaacacctt cgtctccggc
     3421 aactgcgacg tcgtcatcgg catcgtcaac aacaccgtct acgaccccct ccagcccgag
     3481 ctcgactcct tcaaggagga gctcgacaag tacttcaaga accacacctc ccccgacgtc
     3541 gacctcggcg acatctccgg catcaacgcc tccgtcgtca acatccagaa ggagatcgac
     3601 cgcctcaacg aggtcgccaa gaacctcaac gagtccctca tcgacctcca ggagctcggc
     3661 aagtacgagc agtacatcaa gtggccctgg tacatctggc tcggcttcat cgccggcctc
     3721 atcgccatcg tcatggtcac catcatgctc tgctgcatga cctcctgctg ctcctgcctc
     3781 aagggctgct gctcctgcgg ctcctgctgc aagttcgacg aggacgactc cgagcccgtc
     3841 ctcaagggcg tcaagctcca ctacacctaa gctagcagat ctttttccct ctgccaaaaa
     3901 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     3961 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     4021 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     4081 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     4141 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     4201 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     4261 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     4321 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     4381 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     4441 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     4501 tccagtcggg aaacctgtcg
//
