LOCUS       sequence-378873-        3938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3938)
  TITLE     Oka Lab Plasmids 2022
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AV0.1-hSYN-WtSV
FEATURES             Location/Qualifiers
     source          1..3938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        308..755
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     complement(861..881)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    1074..1155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1111..1130)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1165..1184
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   1201..1341
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1201..1330
                     /label=AAV2 ITR
     rep_origin      1416..1871
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1503..1522)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1713..1734
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1888..1907)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2007..2029
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2067..2085)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2153..2257
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2258..2326,2327..3118)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCNAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2476..2495)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3289..3877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3778..3797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgtgg agtcgtgacg tgaattacgt catagggtta gggaggtcct
      181 gtattagagg tcacgtgagt gttttgcgac attttgcgac accatgtggt cacgctgggt
      241 atttaagccc gagtgagcac gcagggtctc cattacgcgt gcggccctgc agagggccct
      301 gcgtatgagt gcaagtgggt tttaggacca ggatgaggcg gggtgggggt gcctacctga
      361 cgaccgaccc cgacccactg gacaagcacc caacccccat tccccaaatt gcgcatcccc
      421 tatcagagag ggggagggga aacaggatgc ggcgaggcgc gtgcgcactg ccagcttcag
      481 caccgcggac agtgccttcg cccccgcctg gcggcgcgcg ccaccgccgc ctcagcactg
      541 aaggcgcgct gacgtcactc gccggtcccc cgcaaactcc ccttcccggc caccttggtc
      601 gcgtccgcgc cgccgccggc ccagccggac cgcaccacgc gaggcgcgag ataggggggc
      661 acgggcgcga ccatctgcgc tgcggcgccg gcgactcagc gctgcctcag tctgcggtgg
      721 gcagcggagg agtcgtgtcg tgcctgagag cgcagtcgag aaggtaccgg atccgctagc
      781 gtttaaacgc ggccgctcga gcaattgaat caacctctgg attacaaaat ttgtgaaaga
      841 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
      901 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
      961 tggttagttc ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     1021 gctcggctgt tgggcactga caattccgtg gtgatcgata agtaagtgac tagaacttgt
     1081 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1141 catttttttc actgcattct agttgtggtt tgtccaaact catctgcacg cgtcggccgc
     1201 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     1261 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     1321 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
     1381 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     1441 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc
     1501 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     1561 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     1621 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     1681 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     1741 actcaactct atctcgggct attcttttga tttataaggg attttgccga tttcggtcta
     1801 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     1861 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     1921 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     1981 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     2041 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     2101 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     2161 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     2221 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     2281 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     2341 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     2401 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     2461 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     2521 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     2581 aaagcatctt acggatggca tgacagtaag agaattatgc aatgctgcca taaccatgag
     2641 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     2701 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     2761 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     2821 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     2881 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     2941 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     3001 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     3061 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     3121 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     3181 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     3241 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3301 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3361 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3421 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3481 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3541 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3601 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3661 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3721 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3781 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3841 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3901 acggttcctg gccttttgct ggccttttgc tcacatgt
//
