LOCUS       sequence-378888-        5321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5321)
  TITLE     Oka Lab Plasmids 2022
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AV0.1-TBG-iCre
FEATURES             Location/Qualifiers
     source          1..5321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        323..732
                     /label=TBG promoter
                     /note="promoter from the human thyroxine-binding globulin 
                     gene (Yan et al., 2012)"
     CDS             778..798
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    1849..2437
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1902..1922)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2320..2331)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2458..2539
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2495..2514)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2549..2568
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   2584..2724
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2584..2713
                     /label=AAV2 ITR
     rep_origin      2799..3254
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2886..2905)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3096..3117
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3271..3290)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3390..3412
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3450..3468)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3536..3640
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3641..3709,3710..4501)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3859..3878)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4672..5260
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5161..5180
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgtgg agtcgtgacg tgaattacgt catagggtta gggaggtcct
      181 gtattagagg tcacgtgagt gttttgcgac attttgcgac accatgtggt cacgctgggt
      241 atttaagccc gagtgagcac gcagggtctc cattacgcgt cgaggggctg gaagctacct
      301 ttgacatcat ttcctctgcg aatgcatgta taatttctac agaacctatt agaaaggatc
      361 acccagcctc tgcttttgta caactttccc ttaaaaaact gccaattcca ctgctgtttg
      421 gcccaatagt gagaactttt tcctgctgcc tcttggtgct tttgcctatg gcccctattc
      481 tgcctgctga agacactctt gccagcatgg acttaaaccc ctccagctct gacaatcctc
      541 tttctctttt gttttacatg aagggtctgg cagccaaagc aatcactcaa agttcaaacc
      601 ttatcatttt ttgctttgtt cctcttggcc ttggttttgt acatcagctt tgaaaatacc
      661 atcccagggt taatgctggg gttaatttat aactaagagt gctctagttt tgcaatacag
      721 gacatgctat aaaaatggaa agataccggt gctagcaagc ttggatccac catggtgccc
      781 aagaagaaga ggaaagtctc caacctgctg actgtgcacc aaaacctgcc tgccctccct
      841 gtggatgcca cctctgatga agtcaggaag aacctgatgg acatgttcag ggacaggcag
      901 gccttctctg aacacacctg gaagatgctc ctgtctgtgt gcagatcctg ggctgcctgg
      961 tgcaagctga acaacaggaa atggttccct gctgaacctg aggatgtgag ggactacctc
     1021 ctgtacctgc aagccagagg cctggctgtg aagaccatcc aacagcacct gggccagctc
     1081 aacatgctgc acaggagatc tggcctgcct cgcccttctg actccaatgc tgtgtccctg
     1141 gtgatgagga gaatcagaaa ggagaatgtg gatgctgggg agagagccaa gcaggccctg
     1201 gcctttgaac gcactgactt tgaccaagtc agatccctga tggagaactc tgacagatgc
     1261 caggacatca ggaacctggc cttcctgggc attgcctaca acaccctgct gcgcattgcc
     1321 gaaattgcca gaatcagagt gaaggacatc tcccgcaccg atggtgggag aatgctgatc
     1381 cacattggca ggaccaagac cctggtgtcc acagctggtg tggagaaggc cctgtccctg
     1441 ggggttacca agctggtgga gagatggatc tctgtgtctg gtgtggctga tgaccccaac
     1501 aactacctgt tctgccgggt cagaaagaat ggtgtggctg ccccttctgc cacctcccaa
     1561 ctgtccaccc gggccctgga agggatcttt gaggccaccc accgcctgat ctatggtgcc
     1621 aaggatgact ctgggcagag atacctggcc tggtctggcc actctgccag agtgggtgct
     1681 gccagggaca tggccagggc tggtgtgtcc atccctgaaa tcatgcaggc tggtggctgg
     1741 accaatgtga acattgtgat gaactacatc agaaacctgg actctgagac tggggccatg
     1801 gtgaggctgc tcgaggatgg ggactgagcg gccgcgaatt ctatcgataa tcaacctctg
     1861 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     1921 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     1981 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     2041 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     2101 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     2161 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     2221 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     2281 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     2341 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     2401 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataagtaag tgactagaac
     2461 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2521 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatctg ccgcgtcggc
     2581 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     2641 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     2701 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     2761 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     2821 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
     2881 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     2941 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3001 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3061 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3121 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
     3181 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     3241 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3301 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     3361 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     3421 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     3481 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3541 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3601 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3661 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3721 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3781 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3841 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3901 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3961 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4021 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4081 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4141 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4201 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4261 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4321 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4381 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4441 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4501 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4561 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4621 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4681 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4741 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4801 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4861 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4921 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4981 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5041 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5101 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5161 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5221 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5281 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
